Binding of [14C]tuberactinomycin O, an antibiotic closely related to viomycin, to the bacterial ribosome
The binding of [14C]tuberactinomycin O, an antibiotic closely related to viomycin, to E. coli ribosomes has been examined by equilibrium dialysis method. The antibiotic has been observed to bind to the 70S ribosome, which possesses two binding sites: one on the 30S ribosomal subunit and another on t...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1978-06, Vol.82 (3), p.971-976 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Misumi, Masarou Tanaka, Nobuo Shiba, Tetsuo |
| description | The binding of [14C]tuberactinomycin O, an antibiotic closely related to viomycin, to E. coli ribosomes has been examined by equilibrium dialysis method. The antibiotic has been observed to bind to the 70S ribosome, which possesses two binding sites: one on the 30S ribosomal subunit and another on the 50S subunit. The affinity for the large subunit is greater than that for the small subunit. The binding to both ribosomal subunits is reversed by viomycin, indicating that tuberactinomycin O and viomycin have the same binding sites on the ribosome. The results seem to be in accordance with the previous finding that viomycin exhibits dual actions on ribosomal function: the inhibition of fMet-tRNAF (initiation) and inhibition of translocation of peptidyl-tRNA. |
| doi_str_mv | 10.1016/0006-291X(78)90878-1 |
| format | Article |
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The antibiotic has been observed to bind to the 70S ribosome, which possesses two binding sites: one on the 30S ribosomal subunit and another on the 50S subunit. The affinity for the large subunit is greater than that for the small subunit. The binding to both ribosomal subunits is reversed by viomycin, indicating that tuberactinomycin O and viomycin have the same binding sites on the ribosome. 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The results seem to be in accordance with the previous finding that viomycin exhibits dual actions on ribosomal function: the inhibition of fMet-tRNAF (initiation) and inhibition of translocation of peptidyl-tRNA.</description><subject>Antibiotics, Antitubercular - metabolism</subject><subject>Binding Sites</subject><subject>Enviomycin - analogs & derivatives</subject><subject>Enviomycin - metabolism</subject><subject>Erythromycin - metabolism</subject><subject>Escherichia coli - metabolism</subject><subject>Molecular Weight</subject><subject>Ribosomes - metabolism</subject><subject>Streptomycin - metabolism</subject><subject>Viomycin - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kFtr3DAQhUVpk26T_oM86Ck0ELczlmzLL4F06Q0CeWmhUIrQZTZRsK1E0gb238dbhzwWBmaGc-bAfIydIHxEwPYTALRV3ePvD50660F1qsJXbIXQQ1UjyNds9WJ5y97lfAeAKNv-kB3UWIMQK3b7OUw-TDc8bvgflOu_ZWspGVfCFMedCxO_PudmmqsEG2IJjrshZhp2PNFgCnleIn8Mi_l8v5Rb4nZOoBTMwFOwMceRjtmbjRkyvX_uR-zX1y8_19-rq-tvP9aXV5UTTVsq6bARToEnKVQjm65RKKwwohbQwTxZqXyvjKsl9A42BqxQ1ngk26ExXhyx0yX3PsWHLeWix5AdDYOZKG6z7mQtW5AwG-VidCnmnGij71MYTdppBL3nq_fw9B6e7pT-x1fjfHbynL-1I_mXowXoLF8sMs0_PgZKOrtAkyMfErmifQz_z38COu2KGA</recordid><startdate>19780614</startdate><enddate>19780614</enddate><creator>Misumi, Masarou</creator><creator>Tanaka, Nobuo</creator><creator>Shiba, Tetsuo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19780614</creationdate><title>Binding of [14C]tuberactinomycin O, an antibiotic closely related to viomycin, to the bacterial ribosome</title><author>Misumi, Masarou ; Tanaka, Nobuo ; Shiba, Tetsuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-4c153c80de43854575813b3a3230703b3b48d98ac2409c0fa0b38bad1eb71aad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Antibiotics, Antitubercular - metabolism</topic><topic>Binding Sites</topic><topic>Enviomycin - analogs & derivatives</topic><topic>Enviomycin - metabolism</topic><topic>Erythromycin - metabolism</topic><topic>Escherichia coli - metabolism</topic><topic>Molecular Weight</topic><topic>Ribosomes - metabolism</topic><topic>Streptomycin - metabolism</topic><topic>Viomycin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Misumi, Masarou</creatorcontrib><creatorcontrib>Tanaka, Nobuo</creatorcontrib><creatorcontrib>Shiba, Tetsuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Misumi, Masarou</au><au>Tanaka, Nobuo</au><au>Shiba, Tetsuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Binding of [14C]tuberactinomycin O, an antibiotic closely related to viomycin, to the bacterial ribosome</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1978-06-14</date><risdate>1978</risdate><volume>82</volume><issue>3</issue><spage>971</spage><epage>976</epage><pages>971-976</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The binding of [14C]tuberactinomycin O, an antibiotic closely related to viomycin, to E. coli ribosomes has been examined by equilibrium dialysis method. 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| ispartof | Biochemical and biophysical research communications, 1978-06, Vol.82 (3), p.971-976 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antibiotics, Antitubercular - metabolism Binding Sites Enviomycin - analogs & derivatives Enviomycin - metabolism Erythromycin - metabolism Escherichia coli - metabolism Molecular Weight Ribosomes - metabolism Streptomycin - metabolism Viomycin - metabolism |
| title | Binding of [14C]tuberactinomycin O, an antibiotic closely related to viomycin, to the bacterial ribosome |
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