Opsonic Effect of CRP on the In Vivo Clearance of Sensitized, Autologous Erythrocytes
We investigated the opsonic capability and requirements of C-reactive protein (CRP) for clearance of autologous erythrocytes sensitized with pneumococcal C-polysaccharide (CPS) in mouse "in vivo" model. Mouse erythrocytes (E) were coated with CPS to provide a binding site for CRP. When E-C...
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| Veröffentlicht in: | Nihon Jinzo Gakkai shi 1982/04/25, Vol.24(4), pp.353-360 |
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| container_issue | 4 |
| container_start_page | 353 |
| container_title | Nihon Jinzo Gakkai shi |
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| creator | Nakayama, Shuei Koide, Hikaru Ohno, Joji DuClos, T.W Mold, C Gewurz, H |
| description | We investigated the opsonic capability and requirements of C-reactive protein (CRP) for clearance of autologous erythrocytes sensitized with pneumococcal C-polysaccharide (CPS) in mouse "in vivo" model. Mouse erythrocytes (E) were coated with CPS to provide a binding site for CRP. When E-CPS or E-CPS-CRP were injected into mice, E-CPS-CRP were preferentially sequested by spleen as compared to E-CPS. The increased splenic sequestration caused by CRP was abolished by pre-treating the mice with cobra venom factor. The removal of CRP from E-CPS-Complement with EDTA showed no increase in splenic sequestration It was also shown that E-CPS-CRP did consume significant amounts of both C4 and C3 in mouse serum. These results demonstrate that CRP mediates opsonic clearance of cells in vivo as well as anti-bodies, indicating that this opsonic effect requires both the complement activation and the continued presence of CRP. |
| doi_str_mv | 10.14842/jpnjnephrol1959.24.353 |
| format | Article |
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Mouse erythrocytes (E) were coated with CPS to provide a binding site for CRP. When E-CPS or E-CPS-CRP were injected into mice, E-CPS-CRP were preferentially sequested by spleen as compared to E-CPS. The increased splenic sequestration caused by CRP was abolished by pre-treating the mice with cobra venom factor. The removal of CRP from E-CPS-Complement with EDTA showed no increase in splenic sequestration It was also shown that E-CPS-CRP did consume significant amounts of both C4 and C3 in mouse serum. 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Mouse erythrocytes (E) were coated with CPS to provide a binding site for CRP. When E-CPS or E-CPS-CRP were injected into mice, E-CPS-CRP were preferentially sequested by spleen as compared to E-CPS. The increased splenic sequestration caused by CRP was abolished by pre-treating the mice with cobra venom factor. The removal of CRP from E-CPS-Complement with EDTA showed no increase in splenic sequestration It was also shown that E-CPS-CRP did consume significant amounts of both C4 and C3 in mouse serum. These results demonstrate that CRP mediates opsonic clearance of cells in vivo as well as anti-bodies, indicating that this opsonic effect requires both the complement activation and the continued presence of CRP.</description><subject>Animals</subject><subject>C-Reactive Protein - pharmacology</subject><subject>Complement C3 - metabolism</subject><subject>Complement C4 - metabolism</subject><subject>CRP</subject><subject>Erythrocytes - metabolism</subject><subject>Female</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><issn>0385-2385</issn><issn>1884-0728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkEtPwzAQhC0Eggr4CQifOJHiZ5weUVWgAgnE6xo5mw1NldohdpHKr8eoFQcus4dvNDsaQs45G3NVKHG17N3SYb8YfMcnejIWaiy13CMjXhQqY0YU-2TEZKEzkeSInIbQVowXhklt1CE5NFywfGJG5O2xD961QGdNgxCpb-j0-Yl6R-MC6dzR9_bL02mHdrAO8Je_oAttbL-xvqTX6-g7_-HXgc6GTUyFYBMxnJCDxnYBT3f3mLzdzF6nd9nD4-18ev2QLXkxiRnw9LSqUifQGmsElifR2EAuhWG5skUCqjaQ24ZDDkJotBI401VdAchjcrHN7Qf_ucYQy1UbALvOOkydSqOEZIyZZDzbGdfVCuuyH9qVHTblbofE77d8GaL9wD9uh9hCh-W_wUuhSrWVtPufCxZ2KNHJH2NvgDU</recordid><startdate>198204</startdate><enddate>198204</enddate><creator>Nakayama, Shuei</creator><creator>Koide, Hikaru</creator><creator>Ohno, Joji</creator><creator>DuClos, T.W</creator><creator>Mold, C</creator><creator>Gewurz, H</creator><general>Japanese Society of Nephrology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>198204</creationdate><title>Opsonic Effect of CRP on the In Vivo Clearance of Sensitized, Autologous Erythrocytes</title><author>Nakayama, Shuei ; Koide, Hikaru ; Ohno, Joji ; DuClos, T.W ; Mold, C ; Gewurz, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j189t-c1ffebb357c55edec06dec5efc6327064a8c554d7c6af1c6c225ea3c105bdbcc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>jpn</language><creationdate>1982</creationdate><topic>Animals</topic><topic>C-Reactive Protein - pharmacology</topic><topic>Complement C3 - metabolism</topic><topic>Complement C4 - metabolism</topic><topic>CRP</topic><topic>Erythrocytes - metabolism</topic><topic>Female</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><toplevel>online_resources</toplevel><creatorcontrib>Nakayama, Shuei</creatorcontrib><creatorcontrib>Koide, Hikaru</creatorcontrib><creatorcontrib>Ohno, Joji</creatorcontrib><creatorcontrib>DuClos, T.W</creatorcontrib><creatorcontrib>Mold, C</creatorcontrib><creatorcontrib>Gewurz, H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Nihon Jinzo Gakkai shi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakayama, Shuei</au><au>Koide, Hikaru</au><au>Ohno, Joji</au><au>DuClos, T.W</au><au>Mold, C</au><au>Gewurz, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Opsonic Effect of CRP on the In Vivo Clearance of Sensitized, Autologous Erythrocytes</atitle><jtitle>Nihon Jinzo Gakkai shi</jtitle><addtitle>Jpn J Nephrol</addtitle><date>1982-04</date><risdate>1982</risdate><volume>24</volume><issue>4</issue><spage>353</spage><epage>360</epage><pages>353-360</pages><issn>0385-2385</issn><eissn>1884-0728</eissn><abstract>We investigated the opsonic capability and requirements of C-reactive protein (CRP) for clearance of autologous erythrocytes sensitized with pneumococcal C-polysaccharide (CPS) in mouse "in vivo" model. Mouse erythrocytes (E) were coated with CPS to provide a binding site for CRP. When E-CPS or E-CPS-CRP were injected into mice, E-CPS-CRP were preferentially sequested by spleen as compared to E-CPS. The increased splenic sequestration caused by CRP was abolished by pre-treating the mice with cobra venom factor. The removal of CRP from E-CPS-Complement with EDTA showed no increase in splenic sequestration It was also shown that E-CPS-CRP did consume significant amounts of both C4 and C3 in mouse serum. These results demonstrate that CRP mediates opsonic clearance of cells in vivo as well as anti-bodies, indicating that this opsonic effect requires both the complement activation and the continued presence of CRP.</abstract><cop>Japan</cop><pub>Japanese Society of Nephrology</pub><pmid>7120697</pmid><doi>10.14842/jpnjnephrol1959.24.353</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0385-2385 |
| ispartof | The Japanese Journal of Nephrology, 1982/04/25, Vol.24(4), pp.353-360 |
| issn | 0385-2385 1884-0728 |
| language | jpn |
| recordid | cdi_proquest_miscellaneous_74230007 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | Animals C-Reactive Protein - pharmacology Complement C3 - metabolism Complement C4 - metabolism CRP Erythrocytes - metabolism Female Mice Mice, Inbred C57BL |
| title | Opsonic Effect of CRP on the In Vivo Clearance of Sensitized, Autologous Erythrocytes |
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