Cloning and Mapping of a Gene for Translational Initiation Factor IF2 in Escherichia coli

A novel method, not relying on genetic complementation of a mutation, was used to clone a gene for translational initiation factor IF2. Two clones from a cosmid library of total Escherichia coli DNA were isolated for their ability to overproduce IF2 in vivo as determined by quantitative immunoblotti...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1982-08, Vol.79 (16), p.5033-5037
Hauptverfasser:	Plumbridge, J. A., Howe, J. G., Springer, M., Touati-Schwartz, D., Hershey, J. W. B., Grunberg-Manago, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage lambda Bacteriophages Chromosome Mapping Chromosomes, Bacterial - ultrastructure Cloning, Molecular Cosmids DNA Escherichia coli Escherichia coli - genetics Gels Gene Expression Regulation Genes Genes, Bacterial Genetic mutation Genetic screening Immunoblotting Libraries Peptide Initiation Factors - genetics Plasmids
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