Evaluation of a defined medium for the production of both thermolabile (LT) and thermostable (ST) enterotoxins of Escherichia coli
The production of thermolabile (LT) enterotoxin was compared in a defined medium reported by Staples et al. (SAG medium) for the production of thermostable (ST) enterotoxin and the Casamino acids-Yeast extract (CAYE) medium. Aliquots were drawn frum cultures of an enterotoxigenic (LT+, ST+) E. coli...
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| Veröffentlicht in: | Medical microbiology and immunology 1982-01, Vol.171 (1), p.43-51 |
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| container_title | Medical microbiology and immunology |
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| creator | Fontes, C F Ricci, L C Oliveira, M S Gatti, M S Pestana de Castro, A F |
| description | The production of thermolabile (LT) enterotoxin was compared in a defined medium reported by Staples et al. (SAG medium) for the production of thermostable (ST) enterotoxin and the Casamino acids-Yeast extract (CAYE) medium. Aliquots were drawn frum cultures of an enterotoxigenic (LT+, ST+) E. coli in both media at different times, growth curves were plotted, and culture filtrates tested for toxin activity. Levels of LT and ST and in the SAG showed that it is as suitable as CAYE for the production of LT. The addition of either glucose (1%) or lincomycin (90 microgram/ml) to SAG medium increased LT levels, but no synergistic effort could be observed if both substances were added concomitantly. Cultures in SAG medium incubated stationarily for 72 h at 37 degrees C yielded more LT than shaking cultures incubated similarly. |
| doi_str_mv | 10.1007/BF02122706 |
| format | Article |
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(SAG medium) for the production of thermostable (ST) enterotoxin and the Casamino acids-Yeast extract (CAYE) medium. Aliquots were drawn frum cultures of an enterotoxigenic (LT+, ST+) E. coli in both media at different times, growth curves were plotted, and culture filtrates tested for toxin activity. Levels of LT and ST and in the SAG showed that it is as suitable as CAYE for the production of LT. The addition of either glucose (1%) or lincomycin (90 microgram/ml) to SAG medium increased LT levels, but no synergistic effort could be observed if both substances were added concomitantly. 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Cultures in SAG medium incubated stationarily for 72 h at 37 degrees C yielded more LT than shaking cultures incubated similarly.</description><subject>Animals</subject><subject>Bacterial Toxins</subject><subject>Culture Media</subject><subject>Enterotoxins - biosynthesis</subject><subject>Escherichia coli</subject><subject>Escherichia coli - growth & development</subject><subject>Escherichia coli Proteins</subject><subject>Glucose - pharmacology</subject><subject>Humans</subject><subject>Lincomycin - pharmacology</subject><subject>Physical Stimulation</subject><subject>Stimulation, Chemical</subject><subject>Swine</subject><issn>0300-8584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAURT2AChQWdiRPCIbAs53EyQhVC0iVGChz9PwR1SiJS-wgWPnlpCLMTFc6Ou9J9xJyzuCGAcjb-xVwxrmE_IAcgwBIiqxIj8hJCG8ATOYcZmSWywxECsfke_mBzYDR-Y76miI1tnadNbS1xg0trX1P49bSXe_NoP805eN2j_vWN6hcY-nVenNNsTMTDRHVnr6M1HbR9j76T9eF_fEy6NFxeuuQat-4U3JYYxPs2ZRz8rpabhaPyfr54Wlxt052XMiYqLRkhYVS6JQBygzrMtNG1iJjOcMcTa4ZmJLXArFAabiqUzBMKVZizrNMzMnl79-xy_tgQ6xaF7RtGuysH0IlU85kWYh_RSbyIhWMjeLFJA5q3Kva9a7F_qua1hU_1nN5tw</recordid><startdate>19820101</startdate><enddate>19820101</enddate><creator>Fontes, C F</creator><creator>Ricci, L C</creator><creator>Oliveira, M S</creator><creator>Gatti, M S</creator><creator>Pestana de Castro, A F</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19820101</creationdate><title>Evaluation of a defined medium for the production of both thermolabile (LT) and thermostable (ST) enterotoxins of Escherichia coli</title><author>Fontes, C F ; Ricci, L C ; Oliveira, M S ; Gatti, M S ; Pestana de Castro, A F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p237t-b4918e093c410a75af95cd7f35161a6ad6c10d92f3aa8a7d2bf40d1bb19a62553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Bacterial Toxins</topic><topic>Culture Media</topic><topic>Enterotoxins - biosynthesis</topic><topic>Escherichia coli</topic><topic>Escherichia coli - growth & development</topic><topic>Escherichia coli Proteins</topic><topic>Glucose - pharmacology</topic><topic>Humans</topic><topic>Lincomycin - pharmacology</topic><topic>Physical Stimulation</topic><topic>Stimulation, Chemical</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fontes, C F</creatorcontrib><creatorcontrib>Ricci, L C</creatorcontrib><creatorcontrib>Oliveira, M S</creatorcontrib><creatorcontrib>Gatti, M S</creatorcontrib><creatorcontrib>Pestana de Castro, A F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Medical microbiology and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fontes, C F</au><au>Ricci, L C</au><au>Oliveira, M S</au><au>Gatti, M S</au><au>Pestana de Castro, A F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a defined medium for the production of both thermolabile (LT) and thermostable (ST) enterotoxins of Escherichia coli</atitle><jtitle>Medical microbiology and immunology</jtitle><addtitle>Med Microbiol Immunol</addtitle><date>1982-01-01</date><risdate>1982</risdate><volume>171</volume><issue>1</issue><spage>43</spage><epage>51</epage><pages>43-51</pages><issn>0300-8584</issn><abstract>The production of thermolabile (LT) enterotoxin was compared in a defined medium reported by Staples et al. 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| identifier | ISSN: 0300-8584 |
| ispartof | Medical microbiology and immunology, 1982-01, Vol.171 (1), p.43-51 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Bacterial Toxins Culture Media Enterotoxins - biosynthesis Escherichia coli Escherichia coli - growth & development Escherichia coli Proteins Glucose - pharmacology Humans Lincomycin - pharmacology Physical Stimulation Stimulation, Chemical Swine |
| title | Evaluation of a defined medium for the production of both thermolabile (LT) and thermostable (ST) enterotoxins of Escherichia coli |
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