Quantitative spectrophotometric assay of renal tissue plasminogen activator
Most determinations of tissue plasminogen activator (TPA) activity have been done with qualitative or semiquantitative variations of the fibrin-overlay technique [1–4]. However, to meaningfully compare renal TPA activities between animal species or in same kidneys under different conditions, a quant...
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| Veröffentlicht in: | Kidney international 1982-07, Vol.22 (1), p.80-83 |
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| creator | Gilboa, Nisan Erdman, Janice Urizar, Rodrigo E. |
| description | Most determinations of tissue plasminogen activator (TPA) activity have been done with qualitative or semiquantitative variations of the fibrin-overlay technique [1–4]. However, to meaningfully compare renal TPA activities between animal species or in same kidneys under different conditions, a quantitative and sensitive assay is required. We are reporting here the development of such an assay based on a two-step reaction: (1) activation of plasminogen (Plg) to plasmin by renal TPA, and (2) quantitation of plasmin by using S2251, a chromogenic substrate highly specific for this enzyme. |
| doi_str_mv | 10.1038/ki.1982.136 |
| format | Article |
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However, to meaningfully compare renal TPA activities between animal species or in same kidneys under different conditions, a quantitative and sensitive assay is required. We are reporting here the development of such an assay based on a two-step reaction: (1) activation of plasminogen (Plg) to plasmin by renal TPA, and (2) quantitation of plasmin by using S2251, a chromogenic substrate highly specific for this enzyme.</description><identifier>ISSN: 0085-2538</identifier><identifier>EISSN: 1523-1755</identifier><identifier>DOI: 10.1038/ki.1982.136</identifier><identifier>PMID: 6214657</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Chromogenic Compounds ; Fibrinolysin - analysis ; Kidney - analysis ; Male ; Oligopeptides ; Plasminogen Activators - analysis ; Rats ; Rats, Inbred Strains ; Spectrophotometry</subject><ispartof>Kidney international, 1982-07, Vol.22 (1), p.80-83</ispartof><rights>1982 International Society of Nephrology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-2b158ad56a8b8325fb45ec994f6252d1a0449c601a3c0ed85f212f24f5b619543</citedby><cites>FETCH-LOGICAL-c391t-2b158ad56a8b8325fb45ec994f6252d1a0449c601a3c0ed85f212f24f5b619543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6214657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gilboa, Nisan</creatorcontrib><creatorcontrib>Erdman, Janice</creatorcontrib><creatorcontrib>Urizar, Rodrigo E.</creatorcontrib><title>Quantitative spectrophotometric assay of renal tissue plasminogen activator</title><title>Kidney international</title><addtitle>Kidney Int</addtitle><description>Most determinations of tissue plasminogen activator (TPA) activity have been done with qualitative or semiquantitative variations of the fibrin-overlay technique [1–4]. 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We are reporting here the development of such an assay based on a two-step reaction: (1) activation of plasminogen (Plg) to plasmin by renal TPA, and (2) quantitation of plasmin by using S2251, a chromogenic substrate highly specific for this enzyme.</description><subject>Animals</subject><subject>Chromogenic Compounds</subject><subject>Fibrinolysin - analysis</subject><subject>Kidney - analysis</subject><subject>Male</subject><subject>Oligopeptides</subject><subject>Plasminogen Activators - analysis</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Spectrophotometry</subject><issn>0085-2538</issn><issn>1523-1755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkM1LwzAYh4Moc05PnsWevEhnPrv0KMMvHIig55CmbzWubWqSDvbf29Gxk6eX8Hvye3kfhC4JnhPM5N3azkku6Zyw7AhNiaAsJQshjtEUYylSKpg8RWch_ODhnTM8QZOMEp6JxRS9vve6jTbqaDeQhA5M9K77dtE1EL01iQ5BbxNXJR5aXSfRhtBD0tU6NLZ1X9Am2gx_dXT-HJ1Uug5wsZ8z9Pn48LF8TldvTy_L-1VqWE5iSgsipC5FpmUhGRVVwQWYPOdVRgUticac5ybDRDODoZSiooRWlFeiyEguOJuhm7G38-63hxBVY4OButYtuD6oBackY1IO4O0IGu9C8FCpzttG-60iWO3UqbVVO3VqUDfQV_vavmigPLB7V0N-Peatjr2HQ762u4qxQYwEDNdvLHgVjIXWQGn9IFaVzv67-Q_yiIbL</recordid><startdate>198207</startdate><enddate>198207</enddate><creator>Gilboa, Nisan</creator><creator>Erdman, Janice</creator><creator>Urizar, Rodrigo E.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198207</creationdate><title>Quantitative spectrophotometric assay of renal tissue plasminogen activator</title><author>Gilboa, Nisan ; Erdman, Janice ; Urizar, Rodrigo E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-2b158ad56a8b8325fb45ec994f6252d1a0449c601a3c0ed85f212f24f5b619543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Chromogenic Compounds</topic><topic>Fibrinolysin - analysis</topic><topic>Kidney - analysis</topic><topic>Male</topic><topic>Oligopeptides</topic><topic>Plasminogen Activators - analysis</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gilboa, Nisan</creatorcontrib><creatorcontrib>Erdman, Janice</creatorcontrib><creatorcontrib>Urizar, Rodrigo E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Kidney international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gilboa, Nisan</au><au>Erdman, Janice</au><au>Urizar, Rodrigo E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative spectrophotometric assay of renal tissue plasminogen activator</atitle><jtitle>Kidney international</jtitle><addtitle>Kidney Int</addtitle><date>1982-07</date><risdate>1982</risdate><volume>22</volume><issue>1</issue><spage>80</spage><epage>83</epage><pages>80-83</pages><issn>0085-2538</issn><eissn>1523-1755</eissn><abstract>Most determinations of tissue plasminogen activator (TPA) activity have been done with qualitative or semiquantitative variations of the fibrin-overlay technique [1–4]. However, to meaningfully compare renal TPA activities between animal species or in same kidneys under different conditions, a quantitative and sensitive assay is required. We are reporting here the development of such an assay based on a two-step reaction: (1) activation of plasminogen (Plg) to plasmin by renal TPA, and (2) quantitation of plasmin by using S2251, a chromogenic substrate highly specific for this enzyme.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6214657</pmid><doi>10.1038/ki.1982.136</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Kidney international, 1982-07, Vol.22 (1), p.80-83 |
| issn | 0085-2538 1523-1755 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Chromogenic Compounds Fibrinolysin - analysis Kidney - analysis Male Oligopeptides Plasminogen Activators - analysis Rats Rats, Inbred Strains Spectrophotometry |
| title | Quantitative spectrophotometric assay of renal tissue plasminogen activator |
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