High-Pressure Liquid Chromatographic Assay of Cloxacillin in Serum and Urine
Two rapid, specific, and sensitive high-pressure liquid chromatographic (HPLC) assays were developed for cloxacillin in serum and urine. A reversed-phase column (RP-8) was selected for use with two different sets of HPLC conditions and sample pretreatment procedures. Cloxacillin extraction efficienc...
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| Veröffentlicht in: | Journal of pharmaceutical sciences 1982-08, Vol.71 (8), p.938-941 |
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| container_title | Journal of pharmaceutical sciences |
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| creator | Teare, F.W. Kwan, R.H. Spino, M. Macleod, S.M. |
| description | Two rapid, specific, and sensitive high-pressure liquid chromatographic (HPLC) assays were developed for cloxacillin in serum and urine. A reversed-phase column (RP-8) was selected for use with two different sets of HPLC conditions and sample pretreatment procedures. Cloxacillin extraction efficiencies are reported from serum and urine. Equations are presented for linear relationships between peak height or peak area ratios of cloxacillin to nafcίllin (internal standard) and the cloxacillin concentration over a range of 0–80 μg/ml. The sensitivity limit of these assays was ~0.3 μg/ml of a standard solution for one method and 0.05 μg/ml for the other HPLC assay. |
| doi_str_mv | 10.1002/jps.2600710826 |
| format | Article |
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A reversed-phase column (RP-8) was selected for use with two different sets of HPLC conditions and sample pretreatment procedures. Cloxacillin extraction efficiencies are reported from serum and urine. Equations are presented for linear relationships between peak height or peak area ratios of cloxacillin to nafcίllin (internal standard) and the cloxacillin concentration over a range of 0–80 μg/ml. 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Pharm. Sci</addtitle><description>Two rapid, specific, and sensitive high-pressure liquid chromatographic (HPLC) assays were developed for cloxacillin in serum and urine. A reversed-phase column (RP-8) was selected for use with two different sets of HPLC conditions and sample pretreatment procedures. Cloxacillin extraction efficiencies are reported from serum and urine. Equations are presented for linear relationships between peak height or peak area ratios of cloxacillin to nafcίllin (internal standard) and the cloxacillin concentration over a range of 0–80 μg/ml. The sensitivity limit of these assays was ~0.3 μg/ml of a standard solution for one method and 0.05 μg/ml for the other HPLC assay.</description><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cloxacillin - analysis</subject><subject>Cloxacillin-high-pressure liquid chromatographic assay</subject><subject>Cloxacillin—high-pressure liquid chromatographic assay, serum, urine, nafcίllin</subject><subject>Cloxacillin—high‐pressure liquid chromatographic assay, serum, urine, nafcillin</subject><subject>high-pressure liquid chromatographic assay</subject><subject>High-pressure liquid chromatography-cloxacillin in serum and urine</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kinetics</subject><subject>Male</subject><subject>nafcillin</subject><subject>Nafcillin - analysis</subject><subject>Nafcillin-cloxacillin</subject><subject>Nafcillin—cloxacillin, high‐pressure liquid chromatographic assay, serum, urine</subject><subject>Nafcίllin—cloxacillin, high-pressure liquid chromatographic assay, serum, urine</subject><subject>Penicillins-high-pressure liquid chromatography of cloxacillin</subject><subject>serum</subject><subject>urine</subject><issn>0022-3549</issn><issn>1520-6017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1Lw0AQhhdRtH5cvQk5eUud3U12k6NUrUpRoYrgZdluJu3WfLS7jdp_byRF8SDCwBzmeV-Yh5BjCn0KwM7mC99nAkBSSJjYIj0aMwgFULlNei3AQh5H6R7Z934OAALieJfsSsqAAu-R0bWdzsIHh943DoORXTY2CwYzV5d6VU-dXsysCc691-ugzoNBUX9oY4vCVkE7Y3RNGegqC56crfCQ7OS68Hi02Qfk6erycXAdju6HN4PzUWgiJkWYmwRj4DTOINWS4ySPzERHTBsNgIxLQfMkT7mhaJI0pROeCMFyLdJYZxHN-AE57XoXrl426FeqtN5gUegK68YrGTFIBE1asN-BxtXeO8zVwtlSu7WioL70qVaf-tHXBk42zc2kxOwb3_hq72l3f7cFrv9pU7cP41_dYZe1foUf31ntXpWQXMbq-W6oKBcvMH5k6qLlk47HVuWbRae8sVgZzKxDs1JZbf964xOcvZ0N</recordid><startdate>198208</startdate><enddate>198208</enddate><creator>Teare, F.W.</creator><creator>Kwan, R.H.</creator><creator>Spino, M.</creator><creator>Macleod, S.M.</creator><general>Elsevier Inc</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198208</creationdate><title>High-Pressure Liquid Chromatographic Assay of Cloxacillin in Serum and Urine</title><author>Teare, F.W. ; Kwan, R.H. ; Spino, M. ; Macleod, S.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4276-fc8e50315d09a73ebf4cba42aca00e23761f8f93c1ec8991b38662fa695ad41d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cloxacillin - analysis</topic><topic>Cloxacillin-high-pressure liquid chromatographic assay</topic><topic>Cloxacillin—high-pressure liquid chromatographic assay, serum, urine, nafcίllin</topic><topic>Cloxacillin—high‐pressure liquid chromatographic assay, serum, urine, nafcillin</topic><topic>high-pressure liquid chromatographic assay</topic><topic>High-pressure liquid chromatography-cloxacillin in serum and urine</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kinetics</topic><topic>Male</topic><topic>nafcillin</topic><topic>Nafcillin - analysis</topic><topic>Nafcillin-cloxacillin</topic><topic>Nafcillin—cloxacillin, high‐pressure liquid chromatographic assay, serum, urine</topic><topic>Nafcίllin—cloxacillin, high-pressure liquid chromatographic assay, serum, urine</topic><topic>Penicillins-high-pressure liquid chromatography of cloxacillin</topic><topic>serum</topic><topic>urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teare, F.W.</creatorcontrib><creatorcontrib>Kwan, R.H.</creatorcontrib><creatorcontrib>Spino, M.</creatorcontrib><creatorcontrib>Macleod, S.M.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teare, F.W.</au><au>Kwan, R.H.</au><au>Spino, M.</au><au>Macleod, S.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-Pressure Liquid Chromatographic Assay of Cloxacillin in Serum and Urine</atitle><jtitle>Journal of pharmaceutical sciences</jtitle><addtitle>J. Pharm. Sci</addtitle><date>1982-08</date><risdate>1982</risdate><volume>71</volume><issue>8</issue><spage>938</spage><epage>941</epage><pages>938-941</pages><issn>0022-3549</issn><eissn>1520-6017</eissn><abstract>Two rapid, specific, and sensitive high-pressure liquid chromatographic (HPLC) assays were developed for cloxacillin in serum and urine. A reversed-phase column (RP-8) was selected for use with two different sets of HPLC conditions and sample pretreatment procedures. Cloxacillin extraction efficiencies are reported from serum and urine. Equations are presented for linear relationships between peak height or peak area ratios of cloxacillin to nafcίllin (internal standard) and the cloxacillin concentration over a range of 0–80 μg/ml. The sensitivity limit of these assays was ~0.3 μg/ml of a standard solution for one method and 0.05 μg/ml for the other HPLC assay.</abstract><cop>Washington</cop><pub>Elsevier Inc</pub><pmid>7120103</pmid><doi>10.1002/jps.2600710826</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of pharmaceutical sciences, 1982-08, Vol.71 (8), p.938-941 |
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| language | eng |
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| source | MEDLINE; Access via Wiley Online Library; Alma/SFX Local Collection |
| subjects | Chromatography, High Pressure Liquid - methods Cloxacillin - analysis Cloxacillin-high-pressure liquid chromatographic assay Cloxacillin—high-pressure liquid chromatographic assay, serum, urine, nafcίllin Cloxacillin—high‐pressure liquid chromatographic assay, serum, urine, nafcillin high-pressure liquid chromatographic assay High-pressure liquid chromatography-cloxacillin in serum and urine Humans Hydrogen-Ion Concentration Kinetics Male nafcillin Nafcillin - analysis Nafcillin-cloxacillin Nafcillin—cloxacillin, high‐pressure liquid chromatographic assay, serum, urine Nafcίllin—cloxacillin, high-pressure liquid chromatographic assay, serum, urine Penicillins-high-pressure liquid chromatography of cloxacillin serum urine |
| title | High-Pressure Liquid Chromatographic Assay of Cloxacillin in Serum and Urine |
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