Total automated method for determination of cholesterol concentration in high density lipoprotein by heparin-magnesium-albumin precipitation
We describe an automated procedure for determination of high-density-lipoprotein cholesterol in serum by use of heparin-MgCl 2-albumin reagent. ‘AutoAnalyzer’ II equipment was used in which high-density lipoprotein was separated from precipitate containing very-low and low-density lipoproteins by fi...
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| Veröffentlicht in: | Clinica chimica acta 1982-08, Vol.123 (3), p.339-345 |
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| container_title | Clinica chimica acta |
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| creator | Tallet, Frank Raichvarg, Denis Lefèvre, Guillaume Canal, Jacques Yonger, Jacques |
| description | We describe an automated procedure for determination of high-density-lipoprotein cholesterol in serum by use of heparin-MgCl
2-albumin reagent. ‘AutoAnalyzer’ II equipment was used in which high-density lipoprotein was separated from precipitate containing very-low and low-density lipoproteins by filtering on-line across a cellulose acetate membrane. Cholesterol concentration was measured by the enzymic method of Allain. Good correlation was obtained using the automated method compared to the heparin-MgCl
2-albumin (
r = 0.99) or sodium phosphotungstate-MgCl
2 procedure (
r = 0.97). Contamination was minimal with a contamination coefficient lower than 2.5%. The method presented was linear (0 to 2.50 mmol/l) and could be applied to lipemic samples with a coefficient of variation better than 3%. This study showed thus that this new procedure was suitable for routine work with a good rate (60 samples/h) in almost any laboratory. |
| doi_str_mv | 10.1016/0009-8981(82)90180-2 |
| format | Article |
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2-albumin reagent. ‘AutoAnalyzer’ II equipment was used in which high-density lipoprotein was separated from precipitate containing very-low and low-density lipoproteins by filtering on-line across a cellulose acetate membrane. Cholesterol concentration was measured by the enzymic method of Allain. Good correlation was obtained using the automated method compared to the heparin-MgCl
2-albumin (
r = 0.99) or sodium phosphotungstate-MgCl
2 procedure (
r = 0.97). Contamination was minimal with a contamination coefficient lower than 2.5%. The method presented was linear (0 to 2.50 mmol/l) and could be applied to lipemic samples with a coefficient of variation better than 3%. This study showed thus that this new procedure was suitable for routine work with a good rate (60 samples/h) in almost any laboratory.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(82)90180-2</identifier><identifier>PMID: 7116650</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Autoanalysis - methods ; Chemical Precipitation ; Cholesterol - blood ; Cholesterol, HDL ; Heparin ; Humans ; Lipoproteins, HDL - blood ; Magnesium ; Magnesium Chloride ; Phosphotungstic Acid ; Serum Albumin, Bovine</subject><ispartof>Clinica chimica acta, 1982-08, Vol.123 (3), p.339-345</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c306t-8faf498192e35285baa21fc214c3134ded4ee6bc411e31293cf5bf093a539ce63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0009898182901802$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7116650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tallet, Frank</creatorcontrib><creatorcontrib>Raichvarg, Denis</creatorcontrib><creatorcontrib>Lefèvre, Guillaume</creatorcontrib><creatorcontrib>Canal, Jacques</creatorcontrib><creatorcontrib>Yonger, Jacques</creatorcontrib><title>Total automated method for determination of cholesterol concentration in high density lipoprotein by heparin-magnesium-albumin precipitation</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>We describe an automated procedure for determination of high-density-lipoprotein cholesterol in serum by use of heparin-MgCl
2-albumin reagent. ‘AutoAnalyzer’ II equipment was used in which high-density lipoprotein was separated from precipitate containing very-low and low-density lipoproteins by filtering on-line across a cellulose acetate membrane. Cholesterol concentration was measured by the enzymic method of Allain. Good correlation was obtained using the automated method compared to the heparin-MgCl
2-albumin (
r = 0.99) or sodium phosphotungstate-MgCl
2 procedure (
r = 0.97). Contamination was minimal with a contamination coefficient lower than 2.5%. The method presented was linear (0 to 2.50 mmol/l) and could be applied to lipemic samples with a coefficient of variation better than 3%. This study showed thus that this new procedure was suitable for routine work with a good rate (60 samples/h) in almost any laboratory.</description><subject>Autoanalysis - methods</subject><subject>Chemical Precipitation</subject><subject>Cholesterol - blood</subject><subject>Cholesterol, HDL</subject><subject>Heparin</subject><subject>Humans</subject><subject>Lipoproteins, HDL - blood</subject><subject>Magnesium</subject><subject>Magnesium Chloride</subject><subject>Phosphotungstic Acid</subject><subject>Serum Albumin, Bovine</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uctq3DAUFSUhnaT9gxa0Cs3CiV72yJtCGJI2EMhmuhayfBWr2JIryYX5h3x0NQ-y7EpI53F1zkXoCyW3lNDmjhDSVrKV9JtkNy2hklTsA1pRueYVFy07Q6t3ykd0mdLvchWkoRfoYk1p09Rkhd62IesR6yWHSWfo8QR5CD22IeIeMsTJeZ1d8DhYbIYwQiqPYcQmeAM-xyPoPB7c61AkPrm8w6ObwxxDhgJ0OzzArKPz1aRfPSS3TJUeu6VY4zmCcbPLB5tP6NzqMcHn03mFfj0-bDc_q-eXH0-b--fKcNLkSlptRQnVMuA1k3WnNaPWMCoMp1z00AuApjOCUuCUtdzYurOk5brmrYGGX6Hro2_54p-lJFKTSwbGUXsIS1Jrwcha1nUhiiPRxJBSBKvm6CYdd4oStV-C2jes9g0rydRhCYoV2deT_9JN0L-LTq0X_PsRhxLyr4OoknFQ-uxdqSOrPrj_D_gH_tKalg</recordid><startdate>19820818</startdate><enddate>19820818</enddate><creator>Tallet, Frank</creator><creator>Raichvarg, Denis</creator><creator>Lefèvre, Guillaume</creator><creator>Canal, Jacques</creator><creator>Yonger, Jacques</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19820818</creationdate><title>Total automated method for determination of cholesterol concentration in high density lipoprotein by heparin-magnesium-albumin precipitation</title><author>Tallet, Frank ; Raichvarg, Denis ; Lefèvre, Guillaume ; Canal, Jacques ; Yonger, Jacques</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-8faf498192e35285baa21fc214c3134ded4ee6bc411e31293cf5bf093a539ce63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Autoanalysis - methods</topic><topic>Chemical Precipitation</topic><topic>Cholesterol - blood</topic><topic>Cholesterol, HDL</topic><topic>Heparin</topic><topic>Humans</topic><topic>Lipoproteins, HDL - blood</topic><topic>Magnesium</topic><topic>Magnesium Chloride</topic><topic>Phosphotungstic Acid</topic><topic>Serum Albumin, Bovine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tallet, Frank</creatorcontrib><creatorcontrib>Raichvarg, Denis</creatorcontrib><creatorcontrib>Lefèvre, Guillaume</creatorcontrib><creatorcontrib>Canal, Jacques</creatorcontrib><creatorcontrib>Yonger, Jacques</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tallet, Frank</au><au>Raichvarg, Denis</au><au>Lefèvre, Guillaume</au><au>Canal, Jacques</au><au>Yonger, Jacques</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Total automated method for determination of cholesterol concentration in high density lipoprotein by heparin-magnesium-albumin precipitation</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1982-08-18</date><risdate>1982</risdate><volume>123</volume><issue>3</issue><spage>339</spage><epage>345</epage><pages>339-345</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>We describe an automated procedure for determination of high-density-lipoprotein cholesterol in serum by use of heparin-MgCl
2-albumin reagent. ‘AutoAnalyzer’ II equipment was used in which high-density lipoprotein was separated from precipitate containing very-low and low-density lipoproteins by filtering on-line across a cellulose acetate membrane. Cholesterol concentration was measured by the enzymic method of Allain. Good correlation was obtained using the automated method compared to the heparin-MgCl
2-albumin (
r = 0.99) or sodium phosphotungstate-MgCl
2 procedure (
r = 0.97). Contamination was minimal with a contamination coefficient lower than 2.5%. The method presented was linear (0 to 2.50 mmol/l) and could be applied to lipemic samples with a coefficient of variation better than 3%. This study showed thus that this new procedure was suitable for routine work with a good rate (60 samples/h) in almost any laboratory.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7116650</pmid><doi>10.1016/0009-8981(82)90180-2</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-8981 |
| ispartof | Clinica chimica acta, 1982-08, Vol.123 (3), p.339-345 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74207855 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Autoanalysis - methods Chemical Precipitation Cholesterol - blood Cholesterol, HDL Heparin Humans Lipoproteins, HDL - blood Magnesium Magnesium Chloride Phosphotungstic Acid Serum Albumin, Bovine |
| title | Total automated method for determination of cholesterol concentration in high density lipoprotein by heparin-magnesium-albumin precipitation |
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