The detection of tritium-labeled ligands and their carrier proteins using a multiwire proportional counter
Two-dimensional immunoelectrophoresis combined with autoradiography is a powerful technique for studying the binding of radiolabeled ligands to their carrier proteins. Tritium-labeled compounds are difficult to detect by autoradiography, yet this isotope is often the radiolabel of choice, because it...
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| Veröffentlicht in: | Anal. Biochem.; (United States) 1982-06, Vol.123 (1), p.1-10 |
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| container_title | Anal. Biochem.; (United States) |
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| creator | Scott, Barbara J. Bateman, J.E. Bradwell, A.R. |
| description | Two-dimensional immunoelectrophoresis combined with autoradiography is a powerful technique for studying the binding of radiolabeled ligands to their carrier proteins. Tritium-labeled compounds are difficult to detect by autoradiography, yet this isotope is often the radiolabel of choice, because it is possible to achieve high specific activity with no loss of biological function. Therefore an electronic detection system called a multiwire proportional counter has been investigated. This has resulted in an increase in detection speed for tritium of several thousand fold over conventional autoradiography and furthermore the results are potentially quantitative. |
| doi_str_mv | 10.1016/0003-2697(82)90615-7 |
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Tritium-labeled compounds are difficult to detect by autoradiography, yet this isotope is often the radiolabel of choice, because it is possible to achieve high specific activity with no loss of biological function. Therefore an electronic detection system called a multiwire proportional counter has been investigated. This has resulted in an increase in detection speed for tritium of several thousand fold over conventional autoradiography and furthermore the results are potentially quantitative.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(82)90615-7</identifier><identifier>PMID: 7114461</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>400103 - Radiometric & Radiochemical Procedures- (-1987) ; 440101 - Radiation Instrumentation- General Detectors or Monitors & Radiometric Instruments ; AUTORADIOGRAPHY ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; Blood Proteins - analysis ; Carrier Proteins - blood ; CHEMICAL ANALYSIS ; Electrodes ; ELECTROPHORESIS ; Humans ; HYDROGEN ISOTOPES ; Immunoelectrophoresis, Two-Dimensional - instrumentation ; INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY ; INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY ; Isotope Labeling ; ISOTOPES ; LABELLED COMPOUNDS ; LIGANDS ; Ligands - analysis ; LIGHT NUCLEI ; MEASURING INSTRUMENTS ; MULTIWIRE PROPORTIONAL CHAMBERS ; NUCLEI ; ODD-EVEN NUCLEI ; ORGANIC COMPOUNDS ; Prednisolone - blood ; PROPORTIONAL COUNTERS ; Protein Binding ; PROTEINS ; QUANTITATIVE CHEMICAL ANALYSIS ; RADIATION DETECTORS ; RADIOCHEMICAL ANALYSIS ; RADIOISOTOPES ; SENSITIVITY ; TRITIUM ; TRITIUM COMPOUNDS ; YEARS LIVING RADIOISOTOPES</subject><ispartof>Anal. 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Biochem.; (United States)</title><addtitle>Anal Biochem</addtitle><description>Two-dimensional immunoelectrophoresis combined with autoradiography is a powerful technique for studying the binding of radiolabeled ligands to their carrier proteins. Tritium-labeled compounds are difficult to detect by autoradiography, yet this isotope is often the radiolabel of choice, because it is possible to achieve high specific activity with no loss of biological function. Therefore an electronic detection system called a multiwire proportional counter has been investigated. This has resulted in an increase in detection speed for tritium of several thousand fold over conventional autoradiography and furthermore the results are potentially quantitative.</description><subject>400103 - Radiometric & Radiochemical Procedures- (-1987)</subject><subject>440101 - Radiation Instrumentation- General Detectors or Monitors & Radiometric Instruments</subject><subject>AUTORADIOGRAPHY</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>Blood Proteins - analysis</subject><subject>Carrier Proteins - blood</subject><subject>CHEMICAL ANALYSIS</subject><subject>Electrodes</subject><subject>ELECTROPHORESIS</subject><subject>Humans</subject><subject>HYDROGEN ISOTOPES</subject><subject>Immunoelectrophoresis, Two-Dimensional - instrumentation</subject><subject>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</subject><subject>INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY</subject><subject>Isotope Labeling</subject><subject>ISOTOPES</subject><subject>LABELLED COMPOUNDS</subject><subject>LIGANDS</subject><subject>Ligands - analysis</subject><subject>LIGHT NUCLEI</subject><subject>MEASURING INSTRUMENTS</subject><subject>MULTIWIRE PROPORTIONAL CHAMBERS</subject><subject>NUCLEI</subject><subject>ODD-EVEN NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>Prednisolone - blood</subject><subject>PROPORTIONAL COUNTERS</subject><subject>Protein Binding</subject><subject>PROTEINS</subject><subject>QUANTITATIVE CHEMICAL ANALYSIS</subject><subject>RADIATION DETECTORS</subject><subject>RADIOCHEMICAL ANALYSIS</subject><subject>RADIOISOTOPES</subject><subject>SENSITIVITY</subject><subject>TRITIUM</subject><subject>TRITIUM COMPOUNDS</subject><subject>YEARS LIVING RADIOISOTOPES</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFP3DAQha0KRBfaf9BKVg8VHELHcdZxLkjVqi1ISFzgbDn2hDVK7K3tgPj3dborjlxsS-_N-M03hHxhcMmAiR8AwKtadO25rC86EGxdtR_IikEnKuDQHZHVm-UjOU3pCYCxZi1OyElbHo1gK_J0v0VqMaPJLngaBpqjy26eqlH3OKKlo3vU3iZaDpq36CI1OkaHke5iyOh8onNy_pFqOs1jdi8u4iLtQlxa6pGaMPuM8RM5HvSY8PPhPiMPv3_db66r27s_N5uft5XhsskV66VsEaHTlg3aDL1dczEwJmsj0BjOOJdrIYQ2gL3s2qEzQvaW9yD4UFvgZ-Tbvm9I2alkXBlua4L3ZUYlamCtFMX0fW8qSf_OmLKaXDI4jtpjmJNqmxp40zXF2OyNJoaUIg5qF92k46tioJY9qAWyWiArWav_e1BtKft66D_3E9q3ogP4ol_tdSwkngvNJSh6g7bgKzltcO9_8A-EnpjW</recordid><startdate>198206</startdate><enddate>198206</enddate><creator>Scott, Barbara J.</creator><creator>Bateman, J.E.</creator><creator>Bradwell, A.R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>198206</creationdate><title>The detection of tritium-labeled ligands and their carrier proteins using a multiwire proportional counter</title><author>Scott, Barbara J. ; Bateman, J.E. ; Bradwell, A.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-1b887ee09ad1facfbd536f1182c6ecc313385666ac0eb897f9c68bd3b063f2d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>400103 - Radiometric & Radiochemical Procedures- (-1987)</topic><topic>440101 - Radiation Instrumentation- General Detectors or Monitors & Radiometric Instruments</topic><topic>AUTORADIOGRAPHY</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>Blood Proteins - analysis</topic><topic>Carrier Proteins - blood</topic><topic>CHEMICAL ANALYSIS</topic><topic>Electrodes</topic><topic>ELECTROPHORESIS</topic><topic>Humans</topic><topic>HYDROGEN ISOTOPES</topic><topic>Immunoelectrophoresis, Two-Dimensional - instrumentation</topic><topic>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</topic><topic>INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY</topic><topic>Isotope Labeling</topic><topic>ISOTOPES</topic><topic>LABELLED COMPOUNDS</topic><topic>LIGANDS</topic><topic>Ligands - analysis</topic><topic>LIGHT NUCLEI</topic><topic>MEASURING INSTRUMENTS</topic><topic>MULTIWIRE PROPORTIONAL CHAMBERS</topic><topic>NUCLEI</topic><topic>ODD-EVEN NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>Prednisolone - blood</topic><topic>PROPORTIONAL COUNTERS</topic><topic>Protein Binding</topic><topic>PROTEINS</topic><topic>QUANTITATIVE CHEMICAL ANALYSIS</topic><topic>RADIATION DETECTORS</topic><topic>RADIOCHEMICAL ANALYSIS</topic><topic>RADIOISOTOPES</topic><topic>SENSITIVITY</topic><topic>TRITIUM</topic><topic>TRITIUM COMPOUNDS</topic><topic>YEARS LIVING RADIOISOTOPES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scott, Barbara J.</creatorcontrib><creatorcontrib>Bateman, J.E.</creatorcontrib><creatorcontrib>Bradwell, A.R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Anal. Biochem.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scott, Barbara J.</au><au>Bateman, J.E.</au><au>Bradwell, A.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The detection of tritium-labeled ligands and their carrier proteins using a multiwire proportional counter</atitle><jtitle>Anal. Biochem.; (United States)</jtitle><addtitle>Anal Biochem</addtitle><date>1982-06</date><risdate>1982</risdate><volume>123</volume><issue>1</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Two-dimensional immunoelectrophoresis combined with autoradiography is a powerful technique for studying the binding of radiolabeled ligands to their carrier proteins. Tritium-labeled compounds are difficult to detect by autoradiography, yet this isotope is often the radiolabel of choice, because it is possible to achieve high specific activity with no loss of biological function. Therefore an electronic detection system called a multiwire proportional counter has been investigated. This has resulted in an increase in detection speed for tritium of several thousand fold over conventional autoradiography and furthermore the results are potentially quantitative.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7114461</pmid><doi>10.1016/0003-2697(82)90615-7</doi><tpages>10</tpages></addata></record> |
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| ispartof | Anal. Biochem.; (United States), 1982-06, Vol.123 (1), p.1-10 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 400103 - Radiometric & Radiochemical Procedures- (-1987) 440101 - Radiation Instrumentation- General Detectors or Monitors & Radiometric Instruments AUTORADIOGRAPHY BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Blood Proteins - analysis Carrier Proteins - blood CHEMICAL ANALYSIS Electrodes ELECTROPHORESIS Humans HYDROGEN ISOTOPES Immunoelectrophoresis, Two-Dimensional - instrumentation INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY Isotope Labeling ISOTOPES LABELLED COMPOUNDS LIGANDS Ligands - analysis LIGHT NUCLEI MEASURING INSTRUMENTS MULTIWIRE PROPORTIONAL CHAMBERS NUCLEI ODD-EVEN NUCLEI ORGANIC COMPOUNDS Prednisolone - blood PROPORTIONAL COUNTERS Protein Binding PROTEINS QUANTITATIVE CHEMICAL ANALYSIS RADIATION DETECTORS RADIOCHEMICAL ANALYSIS RADIOISOTOPES SENSITIVITY TRITIUM TRITIUM COMPOUNDS YEARS LIVING RADIOISOTOPES |
| title | The detection of tritium-labeled ligands and their carrier proteins using a multiwire proportional counter |
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