Inhibition of histone acetylation by N-[2-(S-coenzyme A)acetyl] spermidine amide, a multisubstrate analog

A multisubstrate analog, formed by joining coenzyme A with spermidine through an acetic acid linkage, serves as a strong inhibitor (Ki less than 10(-8) M) of the acetylation of spermidine and histones by histone acetylase purified from calf thymus. In free solutions, this analog inhibited acetylatio...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Journal of biological chemistry 1982-10, Vol.257 (20), p.12165-12169
Hauptverfasser:	Cullis, P M, Wolfenden, R, Cousens, L S, Alberts, B M
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylation Acetyltransferases - antagonists & inhibitors Animals Cattle Histone Acetyltransferases Histones - metabolism Kinetics Saccharomyces cerevisiae Proteins Spermidine - analogs & derivatives Spermidine - metabolism Spermidine - pharmacology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A multisubstrate analog, formed by joining coenzyme A with spermidine through an acetic acid linkage, serves as a strong inhibitor (Ki less than 10(-8) M) of the acetylation of spermidine and histones by histone acetylase purified from calf thymus. In free solutions, this analog inhibited acetylation of the various nuclear histones to a similar extent. In isolated nuclei, this analog was found to inhibit acetylation of histones H2a and H2b very much more strongly than that of histones H3 and H4.
ISSN:	0021-9258 1083-351X
DOI:	10.1016/S0021-9258(18)33695-0