Simultaneous demonstration of microfilaments and intermediate-sized filaments in the lens by double immunofluorescence
Actin- and vimentin filaments (microfilaments and intermediate-sized filaments) are the major components of the lens fiber cytoskeleton. Indirect immunofluorescence suggests a quite different localization of the two kinds of filamentous structures. Actin can only be visualized along the fiber plasma...
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| Veröffentlicht in: | Experimental eye research 1982-10, Vol.35 (4), p.363-369 |
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| container_title | Experimental eye research |
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| creator | Ramaekers, F.C.S. Poels, L.G. Jap, P.H.K. Bloemendal, H. |
| description | Actin- and vimentin filaments (microfilaments and intermediate-sized filaments) are the major components of the lens fiber cytoskeleton. Indirect immunofluorescence suggests a quite different localization of the two kinds of filamentous structures. Actin can only be visualized along the fiber plasma membrane, while vimentin-containing elements seem to fill up the cytoplasmic space. The results are independent of the sequence of staining. |
| doi_str_mv | 10.1016/0014-4835(82)90099-9 |
| format | Article |
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Indirect immunofluorescence suggests a quite different localization of the two kinds of filamentous structures. Actin can only be visualized along the fiber plasma membrane, while vimentin-containing elements seem to fill up the cytoplasmic space. The results are independent of the sequence of staining.</description><identifier>ISSN: 0014-4835</identifier><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1016/0014-4835(82)90099-9</identifier><identifier>PMID: 6754403</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Actins - analysis ; Animals ; Cattle ; Cytoskeleton - analysis ; Electrophoresis ; Fluorescent Antibody Technique ; Immune Sera - immunology ; Intermediate Filament Proteins - analysis ; Lens, Crystalline - ultrastructure ; Vimentin</subject><ispartof>Experimental eye research, 1982-10, Vol.35 (4), p.363-369</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-6cc1a8de97012232b0ddbe608574927dd11e56984d1c18617680f2ca4c413f253</citedby><cites>FETCH-LOGICAL-c357t-6cc1a8de97012232b0ddbe608574927dd11e56984d1c18617680f2ca4c413f253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014483582900999$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6754403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramaekers, F.C.S.</creatorcontrib><creatorcontrib>Poels, L.G.</creatorcontrib><creatorcontrib>Jap, P.H.K.</creatorcontrib><creatorcontrib>Bloemendal, H.</creatorcontrib><title>Simultaneous demonstration of microfilaments and intermediate-sized filaments in the lens by double immunofluorescence</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>Actin- and vimentin filaments (microfilaments and intermediate-sized filaments) are the major components of the lens fiber cytoskeleton. Indirect immunofluorescence suggests a quite different localization of the two kinds of filamentous structures. Actin can only be visualized along the fiber plasma membrane, while vimentin-containing elements seem to fill up the cytoplasmic space. The results are independent of the sequence of staining.</description><subject>Actins - analysis</subject><subject>Animals</subject><subject>Cattle</subject><subject>Cytoskeleton - analysis</subject><subject>Electrophoresis</subject><subject>Fluorescent Antibody Technique</subject><subject>Immune Sera - immunology</subject><subject>Intermediate Filament Proteins - analysis</subject><subject>Lens, Crystalline - ultrastructure</subject><subject>Vimentin</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1rFTEQhoMo9bT6DxRyJfZi7eRjN5sboRS_oNCL6nXIJrMY2SQ1yRbaX-8ez6HeeTUw874z8z6EvGHwgQEbLgCY7OQo-vcjP9cAWnf6Gdkx0EMHAOo52T1JXpLTWn9tXSGVPCEng-qlBLEj97chrkuzCfNaqceYU23FtpATzTONwZU8h8VGTK1SmzwNqWGJ6INt2NXwiJ7-E4RE20-kC6ZKpwfq8zotSEOMa8rzsuaC1WFy-Iq8mO1S8fWxnpEfnz99v_raXd98-XZ1ed050avWDc4xO3rUChjngk_g_YQDjL2SmivvGcN-0KP0zLFxYGoYYebOSieZmHkvzsi7w967kn-vWJuJYftgWQ6BjZIcgAuxCeVBuOWtteBs7kqItjwYBmaP2-xZmj1LM3LzF7fRm-3tcf86bUyeTEe-2_zjYY5byPuAxVQX9gB8KOia8Tn8_8Af43iRHA</recordid><startdate>198210</startdate><enddate>198210</enddate><creator>Ramaekers, F.C.S.</creator><creator>Poels, L.G.</creator><creator>Jap, P.H.K.</creator><creator>Bloemendal, H.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198210</creationdate><title>Simultaneous demonstration of microfilaments and intermediate-sized filaments in the lens by double immunofluorescence</title><author>Ramaekers, F.C.S. ; Poels, L.G. ; Jap, P.H.K. ; Bloemendal, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-6cc1a8de97012232b0ddbe608574927dd11e56984d1c18617680f2ca4c413f253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Actins - analysis</topic><topic>Animals</topic><topic>Cattle</topic><topic>Cytoskeleton - analysis</topic><topic>Electrophoresis</topic><topic>Fluorescent Antibody Technique</topic><topic>Immune Sera - immunology</topic><topic>Intermediate Filament Proteins - analysis</topic><topic>Lens, Crystalline - ultrastructure</topic><topic>Vimentin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramaekers, F.C.S.</creatorcontrib><creatorcontrib>Poels, L.G.</creatorcontrib><creatorcontrib>Jap, P.H.K.</creatorcontrib><creatorcontrib>Bloemendal, H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramaekers, F.C.S.</au><au>Poels, L.G.</au><au>Jap, P.H.K.</au><au>Bloemendal, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous demonstration of microfilaments and intermediate-sized filaments in the lens by double immunofluorescence</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>1982-10</date><risdate>1982</risdate><volume>35</volume><issue>4</issue><spage>363</spage><epage>369</epage><pages>363-369</pages><issn>0014-4835</issn><eissn>1096-0007</eissn><abstract>Actin- and vimentin filaments (microfilaments and intermediate-sized filaments) are the major components of the lens fiber cytoskeleton. Indirect immunofluorescence suggests a quite different localization of the two kinds of filamentous structures. Actin can only be visualized along the fiber plasma membrane, while vimentin-containing elements seem to fill up the cytoplasmic space. The results are independent of the sequence of staining.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>6754403</pmid><doi>10.1016/0014-4835(82)90099-9</doi><tpages>7</tpages></addata></record> |
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| ispartof | Experimental eye research, 1982-10, Vol.35 (4), p.363-369 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Actins - analysis Animals Cattle Cytoskeleton - analysis Electrophoresis Fluorescent Antibody Technique Immune Sera - immunology Intermediate Filament Proteins - analysis Lens, Crystalline - ultrastructure Vimentin |
| title | Simultaneous demonstration of microfilaments and intermediate-sized filaments in the lens by double immunofluorescence |
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