A self determinant recognizing T cell hybridoma
A T cell hybridoma (53(113)) obtained by fusion of BALB/c spleen cells and the BW 5147 lymphoma T cell line is described. This hybridoma recognizes mouse RBC (MRBC) and rat RBC, but not human, rabbit, guinea pig, or SRBC. The culture supernatant possesses hemagglutinating activity for the same indic...
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| Veröffentlicht in: | The Journal of immunology (1950) 1982-09, Vol.129 (3), p.1153-1158 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 129 |
| creator | Juy, D Barbier, E Le Guern, C Cazenave, PA |
| description | A T cell hybridoma (53(113)) obtained by fusion of BALB/c spleen cells and the BW 5147 lymphoma T cell line is described. This hybridoma recognizes mouse RBC (MRBC) and rat RBC, but not human, rabbit, guinea pig, or SRBC. The culture supernatant possesses hemagglutinating activity for the same indicator RBC. In addition to this, 53(113) cells are able to form protein A plaques in the presence of guinea pig complement and normal mouse serum (NMS) or purified mouse immunoglobulins (Ig). Because mouse Ig as well as sonicates from MRBC are able to inhibit the rosettes between the hybridoma cells and the MRBC, and because the sonicates inhibit protein A plaque formation, it seems likely that the same product can recognize a similar determinant expressed on MRBC and mouse Ig. The hypothesis that a 53(113) structure recognizes identical or cross-reactive carbohydrate determinants shared by murine Ig and C is considered. |
| doi_str_mv | 10.4049/jimmunol.129.3.1153 |
| format | Article |
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This hybridoma recognizes mouse RBC (MRBC) and rat RBC, but not human, rabbit, guinea pig, or SRBC. The culture supernatant possesses hemagglutinating activity for the same indicator RBC. In addition to this, 53(113) cells are able to form protein A plaques in the presence of guinea pig complement and normal mouse serum (NMS) or purified mouse immunoglobulins (Ig). Because mouse Ig as well as sonicates from MRBC are able to inhibit the rosettes between the hybridoma cells and the MRBC, and because the sonicates inhibit protein A plaque formation, it seems likely that the same product can recognize a similar determinant expressed on MRBC and mouse Ig. The hypothesis that a 53(113) structure recognizes identical or cross-reactive carbohydrate determinants shared by murine Ig and C is considered.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.129.3.1153</identifier><identifier>PMID: 6179994</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Animals ; Antigens, Surface - analysis ; Cell Membrane - immunology ; Epitopes ; Erythrocytes - immunology ; Hemagglutinins ; Hybridomas - immunology ; Immunoglobulins - immunology ; Mice ; Species Specificity ; T-Lymphocytes - immunology</subject><ispartof>The Journal of immunology (1950), 1982-09, Vol.129 (3), p.1153-1158</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-107d35a1d5cdf156c5c1a1605a763c7d96e987fbc615ddf140ab81c0a941ac483</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6179994$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Juy, D</creatorcontrib><creatorcontrib>Barbier, E</creatorcontrib><creatorcontrib>Le Guern, C</creatorcontrib><creatorcontrib>Cazenave, PA</creatorcontrib><title>A self determinant recognizing T cell hybridoma</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>A T cell hybridoma (53(113)) obtained by fusion of BALB/c spleen cells and the BW 5147 lymphoma T cell line is described. This hybridoma recognizes mouse RBC (MRBC) and rat RBC, but not human, rabbit, guinea pig, or SRBC. The culture supernatant possesses hemagglutinating activity for the same indicator RBC. In addition to this, 53(113) cells are able to form protein A plaques in the presence of guinea pig complement and normal mouse serum (NMS) or purified mouse immunoglobulins (Ig). Because mouse Ig as well as sonicates from MRBC are able to inhibit the rosettes between the hybridoma cells and the MRBC, and because the sonicates inhibit protein A plaque formation, it seems likely that the same product can recognize a similar determinant expressed on MRBC and mouse Ig. The hypothesis that a 53(113) structure recognizes identical or cross-reactive carbohydrate determinants shared by murine Ig and C is considered.</description><subject>Animals</subject><subject>Antigens, Surface - analysis</subject><subject>Cell Membrane - immunology</subject><subject>Epitopes</subject><subject>Erythrocytes - immunology</subject><subject>Hemagglutinins</subject><subject>Hybridomas - immunology</subject><subject>Immunoglobulins - immunology</subject><subject>Mice</subject><subject>Species Specificity</subject><subject>T-Lymphocytes - immunology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkE1LwzAYx4Moc04_gQg96aldnqZJluMYvsHAyzyHNEm3jKadyUqZn96OTfH0HP4v_J8fQveAswIXYrp13ndNW2eQi4xkAJRcoDFQilPGMLtEY4zzPAXO-DW6iXGLMWY4L0ZoxIALIYoxms6TaOsqMXZvg3eNavZJsLpdN-7bNetklWhb18nmUAZnWq9u0VWl6mjvzneCPl-eV4u3dPnx-r6YL1NNON-ngLkhVIGh2lRAmaYaFDBMFWdEcyOYFTNelZoBNYOjwKqcgcZKFKB0MSMT9Hjq3YX2q7NxL72LxymqsW0XJS9ACD58PEHkZNShjTHYSu6C8yocJGB5xCR_MckBkyTyiGlIPZzru9Jb85c5cxn0p5O-cetN74KV0au6Htwg-77_1_QDH0Jydw</recordid><startdate>198209</startdate><enddate>198209</enddate><creator>Juy, D</creator><creator>Barbier, E</creator><creator>Le Guern, C</creator><creator>Cazenave, PA</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198209</creationdate><title>A self determinant recognizing T cell hybridoma</title><author>Juy, D ; Barbier, E ; Le Guern, C ; Cazenave, PA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-107d35a1d5cdf156c5c1a1605a763c7d96e987fbc615ddf140ab81c0a941ac483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Antigens, Surface - analysis</topic><topic>Cell Membrane - immunology</topic><topic>Epitopes</topic><topic>Erythrocytes - immunology</topic><topic>Hemagglutinins</topic><topic>Hybridomas - immunology</topic><topic>Immunoglobulins - immunology</topic><topic>Mice</topic><topic>Species Specificity</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Juy, D</creatorcontrib><creatorcontrib>Barbier, E</creatorcontrib><creatorcontrib>Le Guern, C</creatorcontrib><creatorcontrib>Cazenave, PA</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Juy, D</au><au>Barbier, E</au><au>Le Guern, C</au><au>Cazenave, PA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A self determinant recognizing T cell hybridoma</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1982-09</date><risdate>1982</risdate><volume>129</volume><issue>3</issue><spage>1153</spage><epage>1158</epage><pages>1153-1158</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>A T cell hybridoma (53(113)) obtained by fusion of BALB/c spleen cells and the BW 5147 lymphoma T cell line is described. This hybridoma recognizes mouse RBC (MRBC) and rat RBC, but not human, rabbit, guinea pig, or SRBC. The culture supernatant possesses hemagglutinating activity for the same indicator RBC. In addition to this, 53(113) cells are able to form protein A plaques in the presence of guinea pig complement and normal mouse serum (NMS) or purified mouse immunoglobulins (Ig). Because mouse Ig as well as sonicates from MRBC are able to inhibit the rosettes between the hybridoma cells and the MRBC, and because the sonicates inhibit protein A plaque formation, it seems likely that the same product can recognize a similar determinant expressed on MRBC and mouse Ig. The hypothesis that a 53(113) structure recognizes identical or cross-reactive carbohydrate determinants shared by murine Ig and C is considered.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>6179994</pmid><doi>10.4049/jimmunol.129.3.1153</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of immunology (1950), 1982-09, Vol.129 (3), p.1153-1158 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antigens, Surface - analysis Cell Membrane - immunology Epitopes Erythrocytes - immunology Hemagglutinins Hybridomas - immunology Immunoglobulins - immunology Mice Species Specificity T-Lymphocytes - immunology |
| title | A self determinant recognizing T cell hybridoma |
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