Cell morphology in long-term cultures of normal and abnormal amniotic fluids
The cell morphology of long-term cultures of amniotic fluid cells from 10 fetuses with a neural tube defect (NTD) and three with omphalocele was examined and compared to 30 long-term cultures of normal amniotic fluids as well as a long-term culture of human fetal brain. Cultures from the amniotic fl...
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| Veröffentlicht in: | Human genetics 1982-01, Vol.60 (4), p.310-313 |
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| container_title | Human genetics |
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| creator | Medina-Gómez, P Johnston, T H |
| description | The cell morphology of long-term cultures of amniotic fluid cells from 10 fetuses with a neural tube defect (NTD) and three with omphalocele was examined and compared to 30 long-term cultures of normal amniotic fluids as well as a long-term culture of human fetal brain. Cultures from the amniotic fluids of the fetuses with NTD and omphalocele showed cells with the same general characteristics as normal amniotic fluid cells. However, the cultures of amniotic fluid cells from NTD pregnancies had an additional cell type also seen in fetal brain culture. This was a neuroblast-like cell, with small rounded refractile morphology and long branching processes forming clusters of varying sizes which lay on top of large flat cells. These neuroblast-like cells diminished in number with time in culture and were not present in subcultures. Their possible neuronal origin is discussed. |
| doi_str_mv | 10.1007/BF00569209 |
| format | Article |
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Cultures from the amniotic fluids of the fetuses with NTD and omphalocele showed cells with the same general characteristics as normal amniotic fluid cells. However, the cultures of amniotic fluid cells from NTD pregnancies had an additional cell type also seen in fetal brain culture. This was a neuroblast-like cell, with small rounded refractile morphology and long branching processes forming clusters of varying sizes which lay on top of large flat cells. These neuroblast-like cells diminished in number with time in culture and were not present in subcultures. Their possible neuronal origin is discussed.</description><identifier>ISSN: 0340-6717</identifier><identifier>EISSN: 1432-1203</identifier><identifier>DOI: 10.1007/BF00569209</identifier><identifier>PMID: 6179850</identifier><language>eng</language><publisher>Germany</publisher><subject>alpha-Fetoproteins - analysis ; Amniotic Fluid - cytology ; Brain ; Cell Count ; Cells, Cultured ; Female ; Fetus ; Hernia, Umbilical - pathology ; Humans ; Microscopy, Phase-Contrast ; Neural Tube Defects - pathology ; Neurons - cytology ; Pregnancy ; Time Factors</subject><ispartof>Human genetics, 1982-01, Vol.60 (4), p.310-313</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-79f428e55bff668eeb49827013edef5d3145fd34fbb57bba28e4bd62483571c83</citedby><cites>FETCH-LOGICAL-c282t-79f428e55bff668eeb49827013edef5d3145fd34fbb57bba28e4bd62483571c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6179850$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Medina-Gómez, P</creatorcontrib><creatorcontrib>Johnston, T H</creatorcontrib><title>Cell morphology in long-term cultures of normal and abnormal amniotic fluids</title><title>Human genetics</title><addtitle>Hum Genet</addtitle><description>The cell morphology of long-term cultures of amniotic fluid cells from 10 fetuses with a neural tube defect (NTD) and three with omphalocele was examined and compared to 30 long-term cultures of normal amniotic fluids as well as a long-term culture of human fetal brain. Cultures from the amniotic fluids of the fetuses with NTD and omphalocele showed cells with the same general characteristics as normal amniotic fluid cells. However, the cultures of amniotic fluid cells from NTD pregnancies had an additional cell type also seen in fetal brain culture. This was a neuroblast-like cell, with small rounded refractile morphology and long branching processes forming clusters of varying sizes which lay on top of large flat cells. These neuroblast-like cells diminished in number with time in culture and were not present in subcultures. Their possible neuronal origin is discussed.</description><subject>alpha-Fetoproteins - analysis</subject><subject>Amniotic Fluid - cytology</subject><subject>Brain</subject><subject>Cell Count</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Fetus</subject><subject>Hernia, Umbilical - pathology</subject><subject>Humans</subject><subject>Microscopy, Phase-Contrast</subject><subject>Neural Tube Defects - pathology</subject><subject>Neurons - cytology</subject><subject>Pregnancy</subject><subject>Time Factors</subject><issn>0340-6717</issn><issn>1432-1203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9LwzAYhoMoc04v3oWcPAjVL7-a9KjDqTDwoufSNMmsJM1M2sP-eyubevp44eF9-R6ELgncEgB597ACEGVFoTpCc8IZLQgFdozmwDgUpSTyFJ3l_AlAREXFDM1KIislYI7WS-s9DjFtP6KPmx3ueuxjvykGmwJuRz-MyWYcHe5jCo3HTW9wo39D6Ls4dC12fuxMPkcnrvHZXhzuAr2vHt-Wz8X69elleb8uWqroUMjKcaqsENq5slTWal4pKoEwa6wThhEunGHcaS2k1s3Ecm1KyhUTkrSKLdD1vneb4tdo81CHLrfTI01v45hryYmS09QE3uzBNsWck3X1NnWhSbuaQP2jrv5XN8FXh9ZRB2v-0IMr9g3klWjW</recordid><startdate>19820101</startdate><enddate>19820101</enddate><creator>Medina-Gómez, P</creator><creator>Johnston, T H</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19820101</creationdate><title>Cell morphology in long-term cultures of normal and abnormal amniotic fluids</title><author>Medina-Gómez, P ; Johnston, T H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-79f428e55bff668eeb49827013edef5d3145fd34fbb57bba28e4bd62483571c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>alpha-Fetoproteins - analysis</topic><topic>Amniotic Fluid - cytology</topic><topic>Brain</topic><topic>Cell Count</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Fetus</topic><topic>Hernia, Umbilical - pathology</topic><topic>Humans</topic><topic>Microscopy, Phase-Contrast</topic><topic>Neural Tube Defects - pathology</topic><topic>Neurons - cytology</topic><topic>Pregnancy</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Medina-Gómez, P</creatorcontrib><creatorcontrib>Johnston, T H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Medina-Gómez, P</au><au>Johnston, T H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell morphology in long-term cultures of normal and abnormal amniotic fluids</atitle><jtitle>Human genetics</jtitle><addtitle>Hum Genet</addtitle><date>1982-01-01</date><risdate>1982</risdate><volume>60</volume><issue>4</issue><spage>310</spage><epage>313</epage><pages>310-313</pages><issn>0340-6717</issn><eissn>1432-1203</eissn><abstract>The cell morphology of long-term cultures of amniotic fluid cells from 10 fetuses with a neural tube defect (NTD) and three with omphalocele was examined and compared to 30 long-term cultures of normal amniotic fluids as well as a long-term culture of human fetal brain. Cultures from the amniotic fluids of the fetuses with NTD and omphalocele showed cells with the same general characteristics as normal amniotic fluid cells. However, the cultures of amniotic fluid cells from NTD pregnancies had an additional cell type also seen in fetal brain culture. This was a neuroblast-like cell, with small rounded refractile morphology and long branching processes forming clusters of varying sizes which lay on top of large flat cells. These neuroblast-like cells diminished in number with time in culture and were not present in subcultures. Their possible neuronal origin is discussed.</abstract><cop>Germany</cop><pmid>6179850</pmid><doi>10.1007/BF00569209</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0340-6717 |
| ispartof | Human genetics, 1982-01, Vol.60 (4), p.310-313 |
| issn | 0340-6717 1432-1203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74187282 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | alpha-Fetoproteins - analysis Amniotic Fluid - cytology Brain Cell Count Cells, Cultured Female Fetus Hernia, Umbilical - pathology Humans Microscopy, Phase-Contrast Neural Tube Defects - pathology Neurons - cytology Pregnancy Time Factors |
| title | Cell morphology in long-term cultures of normal and abnormal amniotic fluids |
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