A radiochemical assay for N-acetyl-L-aspartate amidohydrolase (EC 3.5.1.15) and its occurrence in the tissues of the chicken
A simple and rapid radiochemical method for the determination of N-acetyl-L-aspartic acid amidohydrolase (EC 3.5.1.15) activity using ion exchange chromatography has been developed. The activity of this enzyme in the developing brain and some non-nervous tissues of the chicken has been determined. N...
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| Veröffentlicht in: | Life Sci.; (United States) 1978-08, Vol.23 (8), p.791-795 |
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| container_title | Life Sci.; (United States) |
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| creator | D'Adamo, Amedeo F. Wertman, Elon Foster, Faye Schneider, Howard |
| description | A simple and rapid radiochemical method for the determination of N-acetyl-L-aspartic acid amidohydrolase (EC 3.5.1.15) activity using ion exchange chromatography has been developed. The activity of this enzyme in the developing brain and some non-nervous tissues of the chicken has been determined. No activity of the enzyme could be detected in the brains of chick embroys prior to 14 days of gestation; activities gradually increased thereafter to adult levels which are about 60% of that found in the adult rat. In non-nervous system tissues of the adult chicken, activities varied from high levels in the kidney to low levels in heart and breast muscle. Treatment of the homogenates of the adult tissues with a detergent significantly increased the enzyme activity, suggesting that a portion of the enzyme is membrane bound. |
| doi_str_mv | 10.1016/0024-3205(78)90513-1 |
| format | Article |
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The activity of this enzyme in the developing brain and some non-nervous tissues of the chicken has been determined. No activity of the enzyme could be detected in the brains of chick embroys prior to 14 days of gestation; activities gradually increased thereafter to adult levels which are about 60% of that found in the adult rat. In non-nervous system tissues of the adult chicken, activities varied from high levels in the kidney to low levels in heart and breast muscle. Treatment of the homogenates of the adult tissues with a detergent significantly increased the enzyme activity, suggesting that a portion of the enzyme is membrane bound.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/0024-3205(78)90513-1</identifier><identifier>PMID: 703517</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Aging ; Amidohydrolases - metabolism ; ANIMALS ; Aspartic Acid - analogs & derivatives ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; BIRDS ; BODY ; BRAIN ; CARBON 14 ; CARBON ISOTOPES ; CARDIOVASCULAR SYSTEM ; CENTRAL NERVOUS SYSTEM ; CHEMISTRY ; CHICKENS ; CHROMATOGRAPHY ; EMBRYOS ; ENZYME ACTIVITY ; ENZYMES ; EVEN-EVEN NUCLEI ; FOWL ; HEART ; HYDROLASES ; ION EXCHANGE CHROMATOGRAPHY ; ISOTOPES ; Kidney - enzymology ; KIDNEYS ; LIGHT NUCLEI ; Male ; NERVOUS SYSTEM ; Nervous System - enzymology ; NUCLEI ; ORGANS ; RADIOASSAY ; RADIOCHEMISTRY ; RADIOISOTOPES ; SEPARATION PROCESSES ; Tissue Distribution ; TISSUES ; VERTEBRATES ; YEARS LIVING RADIOISOTOPES 550200 -- Biochemistry</subject><ispartof>Life Sci.; (United States), 1978-08, Vol.23 (8), p.791-795</ispartof><rights>1978</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-72cdbb178a654f8af92444057168a7bd3e91d3bbe7ce5cb40114de1bfac2d6843</citedby><cites>FETCH-LOGICAL-c383t-72cdbb178a654f8af92444057168a7bd3e91d3bbe7ce5cb40114de1bfac2d6843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0024320578905131$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/703517$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6803692$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>D'Adamo, Amedeo F.</creatorcontrib><creatorcontrib>Wertman, Elon</creatorcontrib><creatorcontrib>Foster, Faye</creatorcontrib><creatorcontrib>Schneider, Howard</creatorcontrib><creatorcontrib>Univ. of New York, Queens</creatorcontrib><title>A radiochemical assay for N-acetyl-L-aspartate amidohydrolase (EC 3.5.1.15) and its occurrence in the tissues of the chicken</title><title>Life Sci.; (United States)</title><addtitle>Life Sci</addtitle><description>A simple and rapid radiochemical method for the determination of N-acetyl-L-aspartic acid amidohydrolase (EC 3.5.1.15) activity using ion exchange chromatography has been developed. The activity of this enzyme in the developing brain and some non-nervous tissues of the chicken has been determined. No activity of the enzyme could be detected in the brains of chick embroys prior to 14 days of gestation; activities gradually increased thereafter to adult levels which are about 60% of that found in the adult rat. In non-nervous system tissues of the adult chicken, activities varied from high levels in the kidney to low levels in heart and breast muscle. Treatment of the homogenates of the adult tissues with a detergent significantly increased the enzyme activity, suggesting that a portion of the enzyme is membrane bound.</description><subject>Aging</subject><subject>Amidohydrolases - metabolism</subject><subject>ANIMALS</subject><subject>Aspartic Acid - analogs & derivatives</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>BIRDS</subject><subject>BODY</subject><subject>BRAIN</subject><subject>CARBON 14</subject><subject>CARBON ISOTOPES</subject><subject>CARDIOVASCULAR SYSTEM</subject><subject>CENTRAL NERVOUS SYSTEM</subject><subject>CHEMISTRY</subject><subject>CHICKENS</subject><subject>CHROMATOGRAPHY</subject><subject>EMBRYOS</subject><subject>ENZYME ACTIVITY</subject><subject>ENZYMES</subject><subject>EVEN-EVEN NUCLEI</subject><subject>FOWL</subject><subject>HEART</subject><subject>HYDROLASES</subject><subject>ION EXCHANGE CHROMATOGRAPHY</subject><subject>ISOTOPES</subject><subject>Kidney - enzymology</subject><subject>KIDNEYS</subject><subject>LIGHT NUCLEI</subject><subject>Male</subject><subject>NERVOUS SYSTEM</subject><subject>Nervous System - enzymology</subject><subject>NUCLEI</subject><subject>ORGANS</subject><subject>RADIOASSAY</subject><subject>RADIOCHEMISTRY</subject><subject>RADIOISOTOPES</subject><subject>SEPARATION PROCESSES</subject><subject>Tissue Distribution</subject><subject>TISSUES</subject><subject>VERTEBRATES</subject><subject>YEARS LIVING RADIOISOTOPES 550200 -- Biochemistry</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAUxC3Ev6XwDXqwOKD2kMUvtmPnglStSou0ggucLcd-UQzZeLG9SCvx4UmaqkdOljW_NxrNEHIJbAsMmo-M1aLiNZNXSl-3TAKv4BnZgFZtxRoOz8nmCXlN3uT8kzEmpeKvyEvFuAS1IX9vaLI-RDfgITg7UpuzPdM-Jvq1sg7Leaz2lc1Hm4otSO0h-DicfYqjzUivbneUb-UWtiCvqZ08DSXT6NwpJZwc0jDRMiAtIecTzkr_8HVDcL9wekte9HbM-O7xvSA_Pt9-391X-293X3Y3-8pxzUulaue7DpS2jRS9tn1bCyGYVNBoqzrPsQXPuw6VQ-k6wQCER-h662rfaMEvyPvVN-YSTHahoBtcnCZ0xTSa8aatZ-jDCh1T_D1nLeYQssNxtBPGUzZKgK6FbmdQrKBLMeeEvTmmcLDpbICZZRiztG6W1o3S5mEYA_PZ5aP_qTugfzpal5jlT6uMcxF_AqYl51KhD2mJ6WP4v_8_XpWbKg</recordid><startdate>19780828</startdate><enddate>19780828</enddate><creator>D'Adamo, Amedeo F.</creator><creator>Wertman, Elon</creator><creator>Foster, Faye</creator><creator>Schneider, Howard</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19780828</creationdate><title>A radiochemical assay for N-acetyl-L-aspartate amidohydrolase (EC 3.5.1.15) and its occurrence in the tissues of the chicken</title><author>D'Adamo, Amedeo F. ; Wertman, Elon ; Foster, Faye ; Schneider, Howard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-72cdbb178a654f8af92444057168a7bd3e91d3bbe7ce5cb40114de1bfac2d6843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Aging</topic><topic>Amidohydrolases - metabolism</topic><topic>ANIMALS</topic><topic>Aspartic Acid - analogs & derivatives</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>BIRDS</topic><topic>BODY</topic><topic>BRAIN</topic><topic>CARBON 14</topic><topic>CARBON ISOTOPES</topic><topic>CARDIOVASCULAR SYSTEM</topic><topic>CENTRAL NERVOUS SYSTEM</topic><topic>CHEMISTRY</topic><topic>CHICKENS</topic><topic>CHROMATOGRAPHY</topic><topic>EMBRYOS</topic><topic>ENZYME ACTIVITY</topic><topic>ENZYMES</topic><topic>EVEN-EVEN NUCLEI</topic><topic>FOWL</topic><topic>HEART</topic><topic>HYDROLASES</topic><topic>ION EXCHANGE CHROMATOGRAPHY</topic><topic>ISOTOPES</topic><topic>Kidney - enzymology</topic><topic>KIDNEYS</topic><topic>LIGHT NUCLEI</topic><topic>Male</topic><topic>NERVOUS SYSTEM</topic><topic>Nervous System - enzymology</topic><topic>NUCLEI</topic><topic>ORGANS</topic><topic>RADIOASSAY</topic><topic>RADIOCHEMISTRY</topic><topic>RADIOISOTOPES</topic><topic>SEPARATION PROCESSES</topic><topic>Tissue Distribution</topic><topic>TISSUES</topic><topic>VERTEBRATES</topic><topic>YEARS LIVING RADIOISOTOPES 550200 -- Biochemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>D'Adamo, Amedeo F.</creatorcontrib><creatorcontrib>Wertman, Elon</creatorcontrib><creatorcontrib>Foster, Faye</creatorcontrib><creatorcontrib>Schneider, Howard</creatorcontrib><creatorcontrib>Univ. of New York, Queens</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Life Sci.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>D'Adamo, Amedeo F.</au><au>Wertman, Elon</au><au>Foster, Faye</au><au>Schneider, Howard</au><aucorp>Univ. of New York, Queens</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A radiochemical assay for N-acetyl-L-aspartate amidohydrolase (EC 3.5.1.15) and its occurrence in the tissues of the chicken</atitle><jtitle>Life Sci.; (United States)</jtitle><addtitle>Life Sci</addtitle><date>1978-08-28</date><risdate>1978</risdate><volume>23</volume><issue>8</issue><spage>791</spage><epage>795</epage><pages>791-795</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>A simple and rapid radiochemical method for the determination of N-acetyl-L-aspartic acid amidohydrolase (EC 3.5.1.15) activity using ion exchange chromatography has been developed. The activity of this enzyme in the developing brain and some non-nervous tissues of the chicken has been determined. No activity of the enzyme could be detected in the brains of chick embroys prior to 14 days of gestation; activities gradually increased thereafter to adult levels which are about 60% of that found in the adult rat. In non-nervous system tissues of the adult chicken, activities varied from high levels in the kidney to low levels in heart and breast muscle. Treatment of the homogenates of the adult tissues with a detergent significantly increased the enzyme activity, suggesting that a portion of the enzyme is membrane bound.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>703517</pmid><doi>10.1016/0024-3205(78)90513-1</doi><tpages>5</tpages></addata></record> |
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| ispartof | Life Sci.; (United States), 1978-08, Vol.23 (8), p.791-795 |
| issn | 0024-3205 1879-0631 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aging Amidohydrolases - metabolism ANIMALS Aspartic Acid - analogs & derivatives BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES BIRDS BODY BRAIN CARBON 14 CARBON ISOTOPES CARDIOVASCULAR SYSTEM CENTRAL NERVOUS SYSTEM CHEMISTRY CHICKENS CHROMATOGRAPHY EMBRYOS ENZYME ACTIVITY ENZYMES EVEN-EVEN NUCLEI FOWL HEART HYDROLASES ION EXCHANGE CHROMATOGRAPHY ISOTOPES Kidney - enzymology KIDNEYS LIGHT NUCLEI Male NERVOUS SYSTEM Nervous System - enzymology NUCLEI ORGANS RADIOASSAY RADIOCHEMISTRY RADIOISOTOPES SEPARATION PROCESSES Tissue Distribution TISSUES VERTEBRATES YEARS LIVING RADIOISOTOPES 550200 -- Biochemistry |
| title | A radiochemical assay for N-acetyl-L-aspartate amidohydrolase (EC 3.5.1.15) and its occurrence in the tissues of the chicken |
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