Effects of a sedative and a convulsant barbiturate on synaptosomal calcium transport
In vitro additon of pentobarbital inhibited the synaptosomal accumulation of calcium stimulated by potassium depolarization. This inhibition was not due to changes in membrane potentials nor to an enhancement of sodium-calcium exchange, but was at least partly due to an enhanced efflux of calcium. I...
Gespeichert in:
| Veröffentlicht in: | Brain research 1982-06, Vol.242 (1), p.157-163 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 163 |
|---|---|
| container_issue | 1 |
| container_start_page | 157 |
| container_title | Brain research |
| container_volume | 242 |
| creator | Harris, R. Adron Stokes, James A. |
| description | In vitro additon of pentobarbital inhibited the synaptosomal accumulation of calcium stimulated by potassium depolarization. This inhibition was not due to changes in membrane potentials nor to an enhancement of sodium-calcium exchange, but was at least partly due to an enhanced efflux of calcium. In contrast, 5-(2-cyclohexylidene ethyl)-5-ethyl barbituric acid, a convulsant barbiturate, did not alter calcium efflux. Glutamate stimulated synaptosomal calcium accumulation by a depolarization-independent process that was insensitive to the effects of pentobarbital. These results suggest that sedative barbiturates stimulate the efflux of calcium from synaptosomes but convulsant barbiturates do not. In addition, there may be a subpopulation of synaptosomes that contains glutamate-activated calcium channels and is insensitive to pentobarbital. |
| doi_str_mv | 10.1016/0006-8993(82)90506-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74162767</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0006899382905066</els_id><sourcerecordid>74162767</sourcerecordid><originalsourceid>FETCH-LOGICAL-c388t-9403d23ff41e7ef8c1ef7216be5753491cc3cc69516b5b885a30562e36c82deb3</originalsourceid><addsrcrecordid>eNqFkEtLxDAUhYMoOj7-gUJWootqHk2abgQRXyC40XVI0xuItM2YpAP-ezPO4FJXl3PPOffCh9ApJVeUUHlNCJGValt-odhlS0RRcgctqGpYJVlNdtHiN3KADlP6KJLzluyjfUmZYLVcoLd758DmhIPDBifoTfYrwGbqi7RhWs1DMlPGnYmdz3M0GXCYcPqazDKHFEYzYGsG6-cR52imtAwxH6M9Z4YEJ9t5hN4f7t_unqqX18fnu9uXynKlctXWhPeMO1dTaMApS8E1jMoORCN43VJrubWyFWUlOqWE4URIBlxaxXro-BE639xdxvA5Q8p69MnCMJgJwpx0U1PJGtn8G6RC1JwzUoL1JmhjSCmC08voRxO_NCV6TV2vkeo1Uq2Y_qGuZamdbe_P3Qj9b2mLufg3Gx8KjZWHqJP1MFnofSz0dR_83w--AeAhkUM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15543320</pqid></control><display><type>article</type><title>Effects of a sedative and a convulsant barbiturate on synaptosomal calcium transport</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Harris, R. Adron ; Stokes, James A.</creator><creatorcontrib>Harris, R. Adron ; Stokes, James A.</creatorcontrib><description>In vitro additon of pentobarbital inhibited the synaptosomal accumulation of calcium stimulated by potassium depolarization. This inhibition was not due to changes in membrane potentials nor to an enhancement of sodium-calcium exchange, but was at least partly due to an enhanced efflux of calcium. In contrast, 5-(2-cyclohexylidene ethyl)-5-ethyl barbituric acid, a convulsant barbiturate, did not alter calcium efflux. Glutamate stimulated synaptosomal calcium accumulation by a depolarization-independent process that was insensitive to the effects of pentobarbital. These results suggest that sedative barbiturates stimulate the efflux of calcium from synaptosomes but convulsant barbiturates do not. In addition, there may be a subpopulation of synaptosomes that contains glutamate-activated calcium channels and is insensitive to pentobarbital.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(82)90506-6</identifier><identifier>PMID: 6125246</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>5-(2-cyclohexylidene ethyl)-5-ethyl barbituric acid ; Animals ; barbiturates ; Barbiturates - analogs & derivatives ; Barbiturates - pharmacology ; Biological Transport - drug effects ; brain ; Brain - metabolism ; calcium ; Calcium - metabolism ; calcium uptake ; Convulsants - pharmacology ; glutamate ; Glutamates - metabolism ; Glutamic Acid ; Hypnotics and Sedatives - pharmacology ; Male ; membrane potential ; Mice ; Mice, Inbred BALB C ; Onium Compounds - metabolism ; Organophosphorus Compounds ; Pentobarbital - pharmacology ; pentobarbitone ; synaptosomes ; Synaptosomes - metabolism</subject><ispartof>Brain research, 1982-06, Vol.242 (1), p.157-163</ispartof><rights>1982 Elsevier Biomedical Press, Amsterdam</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-9403d23ff41e7ef8c1ef7216be5753491cc3cc69516b5b885a30562e36c82deb3</citedby><cites>FETCH-LOGICAL-c388t-9403d23ff41e7ef8c1ef7216be5753491cc3cc69516b5b885a30562e36c82deb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006899382905066$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6125246$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harris, R. Adron</creatorcontrib><creatorcontrib>Stokes, James A.</creatorcontrib><title>Effects of a sedative and a convulsant barbiturate on synaptosomal calcium transport</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>In vitro additon of pentobarbital inhibited the synaptosomal accumulation of calcium stimulated by potassium depolarization. This inhibition was not due to changes in membrane potentials nor to an enhancement of sodium-calcium exchange, but was at least partly due to an enhanced efflux of calcium. In contrast, 5-(2-cyclohexylidene ethyl)-5-ethyl barbituric acid, a convulsant barbiturate, did not alter calcium efflux. Glutamate stimulated synaptosomal calcium accumulation by a depolarization-independent process that was insensitive to the effects of pentobarbital. These results suggest that sedative barbiturates stimulate the efflux of calcium from synaptosomes but convulsant barbiturates do not. In addition, there may be a subpopulation of synaptosomes that contains glutamate-activated calcium channels and is insensitive to pentobarbital.</description><subject>5-(2-cyclohexylidene ethyl)-5-ethyl barbituric acid</subject><subject>Animals</subject><subject>barbiturates</subject><subject>Barbiturates - analogs & derivatives</subject><subject>Barbiturates - pharmacology</subject><subject>Biological Transport - drug effects</subject><subject>brain</subject><subject>Brain - metabolism</subject><subject>calcium</subject><subject>Calcium - metabolism</subject><subject>calcium uptake</subject><subject>Convulsants - pharmacology</subject><subject>glutamate</subject><subject>Glutamates - metabolism</subject><subject>Glutamic Acid</subject><subject>Hypnotics and Sedatives - pharmacology</subject><subject>Male</subject><subject>membrane potential</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Onium Compounds - metabolism</subject><subject>Organophosphorus Compounds</subject><subject>Pentobarbital - pharmacology</subject><subject>pentobarbitone</subject><subject>synaptosomes</subject><subject>Synaptosomes - metabolism</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAUhYMoOj7-gUJWootqHk2abgQRXyC40XVI0xuItM2YpAP-ezPO4FJXl3PPOffCh9ApJVeUUHlNCJGValt-odhlS0RRcgctqGpYJVlNdtHiN3KADlP6KJLzluyjfUmZYLVcoLd758DmhIPDBifoTfYrwGbqi7RhWs1DMlPGnYmdz3M0GXCYcPqazDKHFEYzYGsG6-cR52imtAwxH6M9Z4YEJ9t5hN4f7t_unqqX18fnu9uXynKlctXWhPeMO1dTaMApS8E1jMoORCN43VJrubWyFWUlOqWE4URIBlxaxXro-BE639xdxvA5Q8p69MnCMJgJwpx0U1PJGtn8G6RC1JwzUoL1JmhjSCmC08voRxO_NCV6TV2vkeo1Uq2Y_qGuZamdbe_P3Qj9b2mLufg3Gx8KjZWHqJP1MFnofSz0dR_83w--AeAhkUM</recordid><startdate>19820617</startdate><enddate>19820617</enddate><creator>Harris, R. Adron</creator><creator>Stokes, James A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19820617</creationdate><title>Effects of a sedative and a convulsant barbiturate on synaptosomal calcium transport</title><author>Harris, R. Adron ; Stokes, James A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-9403d23ff41e7ef8c1ef7216be5753491cc3cc69516b5b885a30562e36c82deb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>5-(2-cyclohexylidene ethyl)-5-ethyl barbituric acid</topic><topic>Animals</topic><topic>barbiturates</topic><topic>Barbiturates - analogs & derivatives</topic><topic>Barbiturates - pharmacology</topic><topic>Biological Transport - drug effects</topic><topic>brain</topic><topic>Brain - metabolism</topic><topic>calcium</topic><topic>Calcium - metabolism</topic><topic>calcium uptake</topic><topic>Convulsants - pharmacology</topic><topic>glutamate</topic><topic>Glutamates - metabolism</topic><topic>Glutamic Acid</topic><topic>Hypnotics and Sedatives - pharmacology</topic><topic>Male</topic><topic>membrane potential</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Onium Compounds - metabolism</topic><topic>Organophosphorus Compounds</topic><topic>Pentobarbital - pharmacology</topic><topic>pentobarbitone</topic><topic>synaptosomes</topic><topic>Synaptosomes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harris, R. Adron</creatorcontrib><creatorcontrib>Stokes, James A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harris, R. Adron</au><au>Stokes, James A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of a sedative and a convulsant barbiturate on synaptosomal calcium transport</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1982-06-17</date><risdate>1982</risdate><volume>242</volume><issue>1</issue><spage>157</spage><epage>163</epage><pages>157-163</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><abstract>In vitro additon of pentobarbital inhibited the synaptosomal accumulation of calcium stimulated by potassium depolarization. This inhibition was not due to changes in membrane potentials nor to an enhancement of sodium-calcium exchange, but was at least partly due to an enhanced efflux of calcium. In contrast, 5-(2-cyclohexylidene ethyl)-5-ethyl barbituric acid, a convulsant barbiturate, did not alter calcium efflux. Glutamate stimulated synaptosomal calcium accumulation by a depolarization-independent process that was insensitive to the effects of pentobarbital. These results suggest that sedative barbiturates stimulate the efflux of calcium from synaptosomes but convulsant barbiturates do not. In addition, there may be a subpopulation of synaptosomes that contains glutamate-activated calcium channels and is insensitive to pentobarbital.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>6125246</pmid><doi>10.1016/0006-8993(82)90506-6</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-8993 |
| ispartof | Brain research, 1982-06, Vol.242 (1), p.157-163 |
| issn | 0006-8993 1872-6240 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74162767 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 5-(2-cyclohexylidene ethyl)-5-ethyl barbituric acid Animals barbiturates Barbiturates - analogs & derivatives Barbiturates - pharmacology Biological Transport - drug effects brain Brain - metabolism calcium Calcium - metabolism calcium uptake Convulsants - pharmacology glutamate Glutamates - metabolism Glutamic Acid Hypnotics and Sedatives - pharmacology Male membrane potential Mice Mice, Inbred BALB C Onium Compounds - metabolism Organophosphorus Compounds Pentobarbital - pharmacology pentobarbitone synaptosomes Synaptosomes - metabolism |
| title | Effects of a sedative and a convulsant barbiturate on synaptosomal calcium transport |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T20%3A30%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20a%20sedative%20and%20a%20convulsant%20barbiturate%20on%20synaptosomal%20calcium%20transport&rft.jtitle=Brain%20research&rft.au=Harris,%20R.%20Adron&rft.date=1982-06-17&rft.volume=242&rft.issue=1&rft.spage=157&rft.epage=163&rft.pages=157-163&rft.issn=0006-8993&rft.eissn=1872-6240&rft_id=info:doi/10.1016/0006-8993(82)90506-6&rft_dat=%3Cproquest_cross%3E74162767%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15543320&rft_id=info:pmid/6125246&rft_els_id=0006899382905066&rfr_iscdi=true |