Studies of the effects of ultraviolet radiation on the structural integrities of ribosomal RNA components of the Escherichia coli 50S ribosomal subunit
The effects of 254-nm radiation on the structural integrities of free and 50S ribosome-bound 5S and 23S ribosomal ribonucleic acids (rRNA) have been elucidated. Irradiation of aqueous solutions of Escherichia coli 50S ribosomes with 253.7-nm radiation results in the formation of single-strand breaks...
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| Veröffentlicht in: | Biochemistry (Easton) 1978-07, Vol.17 (15), p.3152-3162 |
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| creator | Gorelic, Lester Parker, Daryl |
| description | The effects of 254-nm radiation on the structural integrities of free and 50S ribosome-bound 5S and 23S ribosomal ribonucleic acids (rRNA) have been elucidated. Irradiation of aqueous solutions of Escherichia coli 50S ribosomes with 253.7-nm radiation results in the formation of single-strand breaks in double-stranded regions of the 23S rRNA component, but not in rRNA chain scission, and destabilization of the secondary structure of the 23S rRNA toward denaturation. The minimum doses of 253.7-nm radiation required for the first detection of the two effects are 7 x 10(19) quanta for the production of single-strand breaks in double-stranded regions of the 23S rRNA, and less than 2.3 x 10(19) quanta for destabilization of the 23S rRNA secondary structure. Free 23S rRNA is resistant toward photoinduced chain breakage at doses of 253.7-nm radiation up to at least 2.3 x 10(20) and is much less sensitive toward destabilization of secondary structure than ribosome-bound 23S rRNA toward chain breakage, 50S ribosome-bound 5S rRNA is resistant toward chain breakage at doses of 253.7-nm radiation up to at least 2.3 x 10(20) quanta. Ribosome-bound 5S and 23S rRNA are also not photosensitive toward intermolecular 5S/23S rRNA crosslinkage. |
| doi_str_mv | 10.1021/bi00608a033 |
| format | Article |
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Irradiation of aqueous solutions of Escherichia coli 50S ribosomes with 253.7-nm radiation results in the formation of single-strand breaks in double-stranded regions of the 23S rRNA component, but not in rRNA chain scission, and destabilization of the secondary structure of the 23S rRNA toward denaturation. The minimum doses of 253.7-nm radiation required for the first detection of the two effects are 7 x 10(19) quanta for the production of single-strand breaks in double-stranded regions of the 23S rRNA, and less than 2.3 x 10(19) quanta for destabilization of the 23S rRNA secondary structure. Free 23S rRNA is resistant toward photoinduced chain breakage at doses of 253.7-nm radiation up to at least 2.3 x 10(20) and is much less sensitive toward destabilization of secondary structure than ribosome-bound 23S rRNA toward chain breakage, 50S ribosome-bound 5S rRNA is resistant toward chain breakage at doses of 253.7-nm radiation up to at least 2.3 x 10(20) quanta. Ribosome-bound 5S and 23S rRNA are also not photosensitive toward intermolecular 5S/23S rRNA crosslinkage.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00608a033</identifier><identifier>PMID: 359042</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>550200 -- Biochemistry ; 550701 -- Microbiology-- Tracer Techniques ; BACTERIA ; BASIC BIOLOGICAL SCIENCES ; BIOLOGICAL EFFECTS ; BIOLOGICAL RADIATION EFFECTS ; CELL CONSTITUENTS ; DISPERSIONS ; ELECTROMAGNETIC RADIATION ; ESCHERICHIA COLI ; Escherichia coli - radiation effects ; Escherichia coli - ultrastructure ; IN VITRO ; IRRADIATION ; MICROORGANISMS ; MIXTURES ; MOLECULAR STRUCTURE ; Molecular Weight ; Nucleic Acid Conformation ; Nucleic Acid Denaturation ; NUCLEIC ACIDS ; ORGANIC COMPOUNDS ; ORGANOIDS ; RADIATION EFFECTS ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. 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Irradiation of aqueous solutions of Escherichia coli 50S ribosomes with 253.7-nm radiation results in the formation of single-strand breaks in double-stranded regions of the 23S rRNA component, but not in rRNA chain scission, and destabilization of the secondary structure of the 23S rRNA toward denaturation. The minimum doses of 253.7-nm radiation required for the first detection of the two effects are 7 x 10(19) quanta for the production of single-strand breaks in double-stranded regions of the 23S rRNA, and less than 2.3 x 10(19) quanta for destabilization of the 23S rRNA secondary structure. Free 23S rRNA is resistant toward photoinduced chain breakage at doses of 253.7-nm radiation up to at least 2.3 x 10(20) and is much less sensitive toward destabilization of secondary structure than ribosome-bound 23S rRNA toward chain breakage, 50S ribosome-bound 5S rRNA is resistant toward chain breakage at doses of 253.7-nm radiation up to at least 2.3 x 10(20) quanta. Ribosome-bound 5S and 23S rRNA are also not photosensitive toward intermolecular 5S/23S rRNA crosslinkage.</description><subject>550200 -- Biochemistry</subject><subject>550701 -- Microbiology-- Tracer Techniques</subject><subject>BACTERIA</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>CELL CONSTITUENTS</subject><subject>DISPERSIONS</subject><subject>ELECTROMAGNETIC RADIATION</subject><subject>ESCHERICHIA COLI</subject><subject>Escherichia coli - radiation effects</subject><subject>Escherichia coli - ultrastructure</subject><subject>IN VITRO</subject><subject>IRRADIATION</subject><subject>MICROORGANISMS</subject><subject>MIXTURES</subject><subject>MOLECULAR STRUCTURE</subject><subject>Molecular Weight</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic Acid Denaturation</subject><subject>NUCLEIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANOIDS</subject><subject>RADIATION EFFECTS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS 560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)</subject><subject>RADIOINDUCTION</subject><subject>RADIOSENSITIVITY</subject><subject>RIBOSOMES</subject><subject>Ribosomes - radiation effects</subject><subject>Ribosomes - ultrastructure</subject><subject>RNA</subject><subject>RNA, Ribosomal - radiation effects</subject><subject>SOLUTIONS</subject><subject>STRAND BREAKS</subject><subject>ULTRAVIOLET RADIATION</subject><subject>Ultraviolet Rays</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUtv1DAURi3Eayis2LKIuoAFCjhxbI-X1dAHagWoU9haN47NuCT24Acqv4S_i4eMRl0gWbKuzvG98v0Qetngdw1um_e9xZjhJWBCHqBFQ1tcd0LQh2iBC6hbwfBT9CzG21J2mHdP0GNCBe7aBfqzTnmwOlbeVGmjK22MVulfmccU4Jf1o05VgMFCst5V5ey8mEJWKQcYK-uS_h5s2ncJtvfRTwVcfzqplJ-23mmXDhNOo9roYNXGQqGjrShe33sUc5-dTc_RIwNj1C_29xH6enZ6s7qorz6ff1ydXNVAljjVA7DeEE15zxgAB9LTlvVt-ZtQZlgS2mqimBpaDh2mhBjKDSGDMDAwIzglR-h47utjsjIqm7TaKO9c2YJkjHPBl0V6PUvb4H9mHZOcbFR6HMFpn6PkXUN50-7Et7Oogo8xaCO3wU4QfssGy11U8l5UxX61b5v7SQ8Hd86m4HrGNiZ9d6AQfkjGCafy5staXq-_rcQZvZQfiv9m9kFFeetzcGVx_x38F2oZrH4</recordid><startdate>19780725</startdate><enddate>19780725</enddate><creator>Gorelic, Lester</creator><creator>Parker, Daryl</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19780725</creationdate><title>Studies of the effects of ultraviolet radiation on the structural integrities of ribosomal RNA components of the Escherichia coli 50S ribosomal subunit</title><author>Gorelic, Lester ; Parker, Daryl</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a380t-da6bf3e57b66aa7a3b526b29049cfd8352e3c6cd27a40533f57f33d9fad6f9753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>550200 -- Biochemistry</topic><topic>550701 -- Microbiology-- Tracer Techniques</topic><topic>BACTERIA</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>CELL CONSTITUENTS</topic><topic>DISPERSIONS</topic><topic>ELECTROMAGNETIC RADIATION</topic><topic>ESCHERICHIA COLI</topic><topic>Escherichia coli - radiation effects</topic><topic>Escherichia coli - ultrastructure</topic><topic>IN VITRO</topic><topic>IRRADIATION</topic><topic>MICROORGANISMS</topic><topic>MIXTURES</topic><topic>MOLECULAR STRUCTURE</topic><topic>Molecular Weight</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic Acid Denaturation</topic><topic>NUCLEIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANOIDS</topic><topic>RADIATION EFFECTS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIATIONS 560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)</topic><topic>RADIOINDUCTION</topic><topic>RADIOSENSITIVITY</topic><topic>RIBOSOMES</topic><topic>Ribosomes - radiation effects</topic><topic>Ribosomes - ultrastructure</topic><topic>RNA</topic><topic>RNA, Ribosomal - radiation effects</topic><topic>SOLUTIONS</topic><topic>STRAND BREAKS</topic><topic>ULTRAVIOLET RADIATION</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gorelic, Lester</creatorcontrib><creatorcontrib>Parker, Daryl</creatorcontrib><creatorcontrib>Wayne State Univ., Detroit</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gorelic, Lester</au><au>Parker, Daryl</au><aucorp>Wayne State Univ., Detroit</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies of the effects of ultraviolet radiation on the structural integrities of ribosomal RNA components of the Escherichia coli 50S ribosomal subunit</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1978-07-25</date><risdate>1978</risdate><volume>17</volume><issue>15</issue><spage>3152</spage><epage>3162</epage><pages>3152-3162</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>The effects of 254-nm radiation on the structural integrities of free and 50S ribosome-bound 5S and 23S ribosomal ribonucleic acids (rRNA) have been elucidated. Irradiation of aqueous solutions of Escherichia coli 50S ribosomes with 253.7-nm radiation results in the formation of single-strand breaks in double-stranded regions of the 23S rRNA component, but not in rRNA chain scission, and destabilization of the secondary structure of the 23S rRNA toward denaturation. The minimum doses of 253.7-nm radiation required for the first detection of the two effects are 7 x 10(19) quanta for the production of single-strand breaks in double-stranded regions of the 23S rRNA, and less than 2.3 x 10(19) quanta for destabilization of the 23S rRNA secondary structure. Free 23S rRNA is resistant toward photoinduced chain breakage at doses of 253.7-nm radiation up to at least 2.3 x 10(20) and is much less sensitive toward destabilization of secondary structure than ribosome-bound 23S rRNA toward chain breakage, 50S ribosome-bound 5S rRNA is resistant toward chain breakage at doses of 253.7-nm radiation up to at least 2.3 x 10(20) quanta. Ribosome-bound 5S and 23S rRNA are also not photosensitive toward intermolecular 5S/23S rRNA crosslinkage.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>359042</pmid><doi>10.1021/bi00608a033</doi><tpages>11</tpages></addata></record> |
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| ispartof | Biochemistry (Easton), 1978-07, Vol.17 (15), p.3152-3162 |
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| subjects | 550200 -- Biochemistry 550701 -- Microbiology-- Tracer Techniques BACTERIA BASIC BIOLOGICAL SCIENCES BIOLOGICAL EFFECTS BIOLOGICAL RADIATION EFFECTS CELL CONSTITUENTS DISPERSIONS ELECTROMAGNETIC RADIATION ESCHERICHIA COLI Escherichia coli - radiation effects Escherichia coli - ultrastructure IN VITRO IRRADIATION MICROORGANISMS MIXTURES MOLECULAR STRUCTURE Molecular Weight Nucleic Acid Conformation Nucleic Acid Denaturation NUCLEIC ACIDS ORGANIC COMPOUNDS ORGANOIDS RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS 560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987) RADIOINDUCTION RADIOSENSITIVITY RIBOSOMES Ribosomes - radiation effects Ribosomes - ultrastructure RNA RNA, Ribosomal - radiation effects SOLUTIONS STRAND BREAKS ULTRAVIOLET RADIATION Ultraviolet Rays |
| title | Studies of the effects of ultraviolet radiation on the structural integrities of ribosomal RNA components of the Escherichia coli 50S ribosomal subunit |
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