Identification of vasopressor phospholipid in crude soybean lecithin
The vasopressor phospholipid in crude soybean lecithin was isolated by column chromatography on Sephadex LH‐20. It represented 0.1% of crude soybean lecithin. The isolated phospholipid was identified to be lysophosphatidic acid by gas chromatography‐mass spectrometry analysis of TMS‐deacylated produ...
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| Veröffentlicht in: | Lipids 1978-07, Vol.13 (7), p.468-472 |
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| creator | Tokumura, Akira Fukuzawa, Kenji Akamatsu, Yasue Yamada, Sadaji Suzuki, Tetsuya Tsukatani, Hiroaki |
| description | The vasopressor phospholipid in crude soybean lecithin was isolated by column chromatography on Sephadex LH‐20. It represented 0.1% of crude soybean lecithin. The isolated phospholipid was identified to be lysophosphatidic acid by gas chromatography‐mass spectrometry analysis of TMS‐deacylated product and acetolysis product. Nuclear magnetic resonance analysis favored the 1‐monoacyl isomer over the 2‐isomer. By enzymic determination with L‐3‐glycerophosphate dehydrogenase, the isolated phospholipid was identified as 1‐monoacyl‐L‐3‐glycerophosphate. Gas chromatographic examination revealed that it was composed of a large percentage of unsaturated fatty acids, especially linoleic acid. The activity of isolated lysophosphatidic acid was slightly less than that of synthetic 1‐linoleoyl‐L‐3‐glycerophosphate. |
| doi_str_mv | 10.1007/BF02533615 |
| format | Article |
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It represented 0.1% of crude soybean lecithin. The isolated phospholipid was identified to be lysophosphatidic acid by gas chromatography‐mass spectrometry analysis of TMS‐deacylated product and acetolysis product. Nuclear magnetic resonance analysis favored the 1‐monoacyl isomer over the 2‐isomer. By enzymic determination with L‐3‐glycerophosphate dehydrogenase, the isolated phospholipid was identified as 1‐monoacyl‐L‐3‐glycerophosphate. Gas chromatographic examination revealed that it was composed of a large percentage of unsaturated fatty acids, especially linoleic acid. The activity of isolated lysophosphatidic acid was slightly less than that of synthetic 1‐linoleoyl‐L‐3‐glycerophosphate.</description><identifier>ISSN: 0024-4201</identifier><identifier>EISSN: 1558-9307</identifier><identifier>DOI: 10.1007/BF02533615</identifier><identifier>PMID: 567731</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer‐Verlag</publisher><subject>Animals ; Blood Pressure - drug effects ; Chromatography, Thin Layer ; Glycine max ; Mass Spectrometry ; Phosphatidylcholines - isolation & purification ; Phospholipids - isolation & purification ; Phospholipids - pharmacology ; Rats ; Vasoconstrictor Agents - isolation & purification</subject><ispartof>Lipids, 1978-07, Vol.13 (7), p.468-472</ispartof><rights>1978 American Oil Chemists' Society (AOCS)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3888-2fc15bcd0f8ced93df62d1a97f2db57fe29ca705a0fe9856a7da8716db2d29e53</citedby><cites>FETCH-LOGICAL-c3888-2fc15bcd0f8ced93df62d1a97f2db57fe29ca705a0fe9856a7da8716db2d29e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/567731$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tokumura, Akira</creatorcontrib><creatorcontrib>Fukuzawa, Kenji</creatorcontrib><creatorcontrib>Akamatsu, Yasue</creatorcontrib><creatorcontrib>Yamada, Sadaji</creatorcontrib><creatorcontrib>Suzuki, Tetsuya</creatorcontrib><creatorcontrib>Tsukatani, Hiroaki</creatorcontrib><title>Identification of vasopressor phospholipid in crude soybean lecithin</title><title>Lipids</title><addtitle>Lipids</addtitle><description>The vasopressor phospholipid in crude soybean lecithin was isolated by column chromatography on Sephadex LH‐20. It represented 0.1% of crude soybean lecithin. The isolated phospholipid was identified to be lysophosphatidic acid by gas chromatography‐mass spectrometry analysis of TMS‐deacylated product and acetolysis product. Nuclear magnetic resonance analysis favored the 1‐monoacyl isomer over the 2‐isomer. By enzymic determination with L‐3‐glycerophosphate dehydrogenase, the isolated phospholipid was identified as 1‐monoacyl‐L‐3‐glycerophosphate. Gas chromatographic examination revealed that it was composed of a large percentage of unsaturated fatty acids, especially linoleic acid. The activity of isolated lysophosphatidic acid was slightly less than that of synthetic 1‐linoleoyl‐L‐3‐glycerophosphate.</description><subject>Animals</subject><subject>Blood Pressure - drug effects</subject><subject>Chromatography, Thin Layer</subject><subject>Glycine max</subject><subject>Mass Spectrometry</subject><subject>Phosphatidylcholines - isolation & purification</subject><subject>Phospholipids - isolation & purification</subject><subject>Phospholipids - pharmacology</subject><subject>Rats</subject><subject>Vasoconstrictor Agents - isolation & purification</subject><issn>0024-4201</issn><issn>1558-9307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtPwzAUhS3EqxQWZoZMDEgBP-LYGWlLoVIlGGCOHPtaNUrjYCeg_nuCUsHGcHV0dT59w0HokuBbgrG4my0x5YzlhB-gCeFcpgXD4hBNMKZZmlFMTtFZjO_DS7KCn6BjngvByAQtVgaazlmnVed8k3ibfKro2wAx-pC0Gx-Hq13rTOKaRIfeQBL9rgLVJDVo121cc46OrKojXOxzit6WD6_zp3T9_Lia369TzaSUKbWa8EobbKUGUzBjc2qIKoSlpuLCAi20EpgrbKGQPFfCKClIbipqaAGcTdH16G2D_-ghduXWRQ11rRrwfSxFRjLGBB3AmxHUwccYwJZtcFsVdiXB5c9i5d9iA3y1t_bVFswvOk401Hisv1wNu39E5Xr1ssBZLtk3e7V1Cg</recordid><startdate>197807</startdate><enddate>197807</enddate><creator>Tokumura, Akira</creator><creator>Fukuzawa, Kenji</creator><creator>Akamatsu, Yasue</creator><creator>Yamada, Sadaji</creator><creator>Suzuki, Tetsuya</creator><creator>Tsukatani, Hiroaki</creator><general>Springer‐Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197807</creationdate><title>Identification of vasopressor phospholipid in crude soybean lecithin</title><author>Tokumura, Akira ; Fukuzawa, Kenji ; Akamatsu, Yasue ; Yamada, Sadaji ; Suzuki, Tetsuya ; Tsukatani, Hiroaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3888-2fc15bcd0f8ced93df62d1a97f2db57fe29ca705a0fe9856a7da8716db2d29e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Animals</topic><topic>Blood Pressure - drug effects</topic><topic>Chromatography, Thin Layer</topic><topic>Glycine max</topic><topic>Mass Spectrometry</topic><topic>Phosphatidylcholines - isolation & purification</topic><topic>Phospholipids - isolation & purification</topic><topic>Phospholipids - pharmacology</topic><topic>Rats</topic><topic>Vasoconstrictor Agents - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tokumura, Akira</creatorcontrib><creatorcontrib>Fukuzawa, Kenji</creatorcontrib><creatorcontrib>Akamatsu, Yasue</creatorcontrib><creatorcontrib>Yamada, Sadaji</creatorcontrib><creatorcontrib>Suzuki, Tetsuya</creatorcontrib><creatorcontrib>Tsukatani, Hiroaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tokumura, Akira</au><au>Fukuzawa, Kenji</au><au>Akamatsu, Yasue</au><au>Yamada, Sadaji</au><au>Suzuki, Tetsuya</au><au>Tsukatani, Hiroaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of vasopressor phospholipid in crude soybean lecithin</atitle><jtitle>Lipids</jtitle><addtitle>Lipids</addtitle><date>1978-07</date><risdate>1978</risdate><volume>13</volume><issue>7</issue><spage>468</spage><epage>472</epage><pages>468-472</pages><issn>0024-4201</issn><eissn>1558-9307</eissn><abstract>The vasopressor phospholipid in crude soybean lecithin was isolated by column chromatography on Sephadex LH‐20. It represented 0.1% of crude soybean lecithin. The isolated phospholipid was identified to be lysophosphatidic acid by gas chromatography‐mass spectrometry analysis of TMS‐deacylated product and acetolysis product. Nuclear magnetic resonance analysis favored the 1‐monoacyl isomer over the 2‐isomer. By enzymic determination with L‐3‐glycerophosphate dehydrogenase, the isolated phospholipid was identified as 1‐monoacyl‐L‐3‐glycerophosphate. Gas chromatographic examination revealed that it was composed of a large percentage of unsaturated fatty acids, especially linoleic acid. The activity of isolated lysophosphatidic acid was slightly less than that of synthetic 1‐linoleoyl‐L‐3‐glycerophosphate.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer‐Verlag</pub><pmid>567731</pmid><doi>10.1007/BF02533615</doi><tpages>5</tpages></addata></record> |
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| ispartof | Lipids, 1978-07, Vol.13 (7), p.468-472 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Blood Pressure - drug effects Chromatography, Thin Layer Glycine max Mass Spectrometry Phosphatidylcholines - isolation & purification Phospholipids - isolation & purification Phospholipids - pharmacology Rats Vasoconstrictor Agents - isolation & purification |
| title | Identification of vasopressor phospholipid in crude soybean lecithin |
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