Stimulation of Fibroblast Biosynthetic Activity by Serum of Patients with Pretibial Myxedema
Skin fibroblasts from the shoulder and lower extremities of normal individuals, as well as from patients with pretibial myxedema (PTM) were grown in culture. When cells reached the monolayer stage, they were labeled with 3H-glucosamine and tested for hyaluronic acid synthesis in the presence of eith...
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| Veröffentlicht in: | Journal of investigative dermatology 1978-07, Vol.71 (1), p.12-17 |
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| container_title | Journal of investigative dermatology |
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| creator | Cheung, Herman S Nicoloff, John T Kamiel, Michael B Spolter, Leonard Nimni, Marcel E |
| description | Skin fibroblasts from the shoulder and lower extremities of normal individuals, as well as from patients with pretibial myxedema (PTM) were grown in culture. When cells reached the monolayer stage, they were labeled with 3H-glucosamine and tested for hyaluronic acid synthesis in the presence of either serum from PTM patients or normal human serum. All the fibroblasts from the pretibial area synthesized 2 to 3 times more hyaluronic acid when incubated with PTM sera than when incubated in normal human serum. Fibroblasts cultured from skin of the back or prepuce did not respond to PTM sera. This heat-stable, protease-sensitive, and dialyzable, fibroblast-stimulating factor is not a 7Sγ-globulin. The enhanced sensitivity to PTM sera exhibited by fibroblasts from the lower extremities may explain why the lesions in this disease are restricted primarily to that area. |
| doi_str_mv | 10.1111/1523-1747.ep12543646 |
| format | Article |
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When cells reached the monolayer stage, they were labeled with 3H-glucosamine and tested for hyaluronic acid synthesis in the presence of either serum from PTM patients or normal human serum. All the fibroblasts from the pretibial area synthesized 2 to 3 times more hyaluronic acid when incubated with PTM sera than when incubated in normal human serum. Fibroblasts cultured from skin of the back or prepuce did not respond to PTM sera. This heat-stable, protease-sensitive, and dialyzable, fibroblast-stimulating factor is not a 7Sγ-globulin. The enhanced sensitivity to PTM sera exhibited by fibroblasts from the lower extremities may explain why the lesions in this disease are restricted primarily to that area.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/1523-1747.ep12543646</identifier><identifier>PMID: 355562</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Blood ; Cells, Cultured ; Chemical Phenomena ; Chemistry ; Connective Tissue - metabolism ; Fibroblasts - metabolism ; Graves Disease - blood ; Humans ; Hyaluronic Acid - biosynthesis ; Leg Dermatoses - blood ; Molecular Conformation ; Myxedema - blood ; Skin - ultrastructure ; Stimulation, Chemical</subject><ispartof>Journal of investigative dermatology, 1978-07, Vol.71 (1), p.12-17</ispartof><rights>1978 The Williams & Wilkins Co.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-752a9e8d89a14ec0b0f2c86461f9bb83a4f8efdf46ec3345badfd5f9a65910d93</citedby><cites>FETCH-LOGICAL-c424t-752a9e8d89a14ec0b0f2c86461f9bb83a4f8efdf46ec3345badfd5f9a65910d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/355562$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheung, Herman S</creatorcontrib><creatorcontrib>Nicoloff, John T</creatorcontrib><creatorcontrib>Kamiel, Michael B</creatorcontrib><creatorcontrib>Spolter, Leonard</creatorcontrib><creatorcontrib>Nimni, Marcel E</creatorcontrib><title>Stimulation of Fibroblast Biosynthetic Activity by Serum of Patients with Pretibial Myxedema</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Skin fibroblasts from the shoulder and lower extremities of normal individuals, as well as from patients with pretibial myxedema (PTM) were grown in culture. When cells reached the monolayer stage, they were labeled with 3H-glucosamine and tested for hyaluronic acid synthesis in the presence of either serum from PTM patients or normal human serum. All the fibroblasts from the pretibial area synthesized 2 to 3 times more hyaluronic acid when incubated with PTM sera than when incubated in normal human serum. Fibroblasts cultured from skin of the back or prepuce did not respond to PTM sera. This heat-stable, protease-sensitive, and dialyzable, fibroblast-stimulating factor is not a 7Sγ-globulin. The enhanced sensitivity to PTM sera exhibited by fibroblasts from the lower extremities may explain why the lesions in this disease are restricted primarily to that area.</description><subject>Blood</subject><subject>Cells, Cultured</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Connective Tissue - metabolism</subject><subject>Fibroblasts - metabolism</subject><subject>Graves Disease - blood</subject><subject>Humans</subject><subject>Hyaluronic Acid - biosynthesis</subject><subject>Leg Dermatoses - blood</subject><subject>Molecular Conformation</subject><subject>Myxedema - blood</subject><subject>Skin - ultrastructure</subject><subject>Stimulation, Chemical</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kElrHDEQhUVwEk8m-Qc26JRbO5Ja6uVisI03cIjBCeQQEFpKWKGXiaR23P8-atpMbq5LQb33qqgPoSNKTmiuL1SwsqA1r09gR5ngZcWrN2izHx-gDSGMFYywn4foQ4y_CaEVF8179K4UQlRsg349JN9PnUp-HPDo8JXXYdSdigmf-zHOQ3qE5A0-M8k_-TRjPeMHCFO_mO9zDIYU8V-fHvF9yE7tVYe_zs9goVcf0VunugifXvoW_bi6_H5xU9x9u769OLsrDGc8FbVgqoXGNq2iHAzRxDHT5Geoa7VuSsVdA846XoEpSy60ss4K16pKtJTYttyiz-veXRj_TBCT7H000HVqgHGKsuaUkzZj2SK-Gk0YYwzg5C74XoVZUiIXpnKBJxd48j_THDt62T_pHuw-tELM8vEqDypNAfayqDLteomfrjpkBk8egowmczNgfQCTpB396_f_AVphkZ4</recordid><startdate>197807</startdate><enddate>197807</enddate><creator>Cheung, Herman S</creator><creator>Nicoloff, John T</creator><creator>Kamiel, Michael B</creator><creator>Spolter, Leonard</creator><creator>Nimni, Marcel E</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197807</creationdate><title>Stimulation of Fibroblast Biosynthetic Activity by Serum of Patients with Pretibial Myxedema</title><author>Cheung, Herman S ; Nicoloff, John T ; Kamiel, Michael B ; Spolter, Leonard ; Nimni, Marcel E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-752a9e8d89a14ec0b0f2c86461f9bb83a4f8efdf46ec3345badfd5f9a65910d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Blood</topic><topic>Cells, Cultured</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Connective Tissue - metabolism</topic><topic>Fibroblasts - metabolism</topic><topic>Graves Disease - blood</topic><topic>Humans</topic><topic>Hyaluronic Acid - biosynthesis</topic><topic>Leg Dermatoses - blood</topic><topic>Molecular Conformation</topic><topic>Myxedema - blood</topic><topic>Skin - ultrastructure</topic><topic>Stimulation, Chemical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheung, Herman S</creatorcontrib><creatorcontrib>Nicoloff, John T</creatorcontrib><creatorcontrib>Kamiel, Michael B</creatorcontrib><creatorcontrib>Spolter, Leonard</creatorcontrib><creatorcontrib>Nimni, Marcel E</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheung, Herman S</au><au>Nicoloff, John T</au><au>Kamiel, Michael B</au><au>Spolter, Leonard</au><au>Nimni, Marcel E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stimulation of Fibroblast Biosynthetic Activity by Serum of Patients with Pretibial Myxedema</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>1978-07</date><risdate>1978</risdate><volume>71</volume><issue>1</issue><spage>12</spage><epage>17</epage><pages>12-17</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><abstract>Skin fibroblasts from the shoulder and lower extremities of normal individuals, as well as from patients with pretibial myxedema (PTM) were grown in culture. When cells reached the monolayer stage, they were labeled with 3H-glucosamine and tested for hyaluronic acid synthesis in the presence of either serum from PTM patients or normal human serum. All the fibroblasts from the pretibial area synthesized 2 to 3 times more hyaluronic acid when incubated with PTM sera than when incubated in normal human serum. Fibroblasts cultured from skin of the back or prepuce did not respond to PTM sera. This heat-stable, protease-sensitive, and dialyzable, fibroblast-stimulating factor is not a 7Sγ-globulin. The enhanced sensitivity to PTM sera exhibited by fibroblasts from the lower extremities may explain why the lesions in this disease are restricted primarily to that area.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>355562</pmid><doi>10.1111/1523-1747.ep12543646</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of investigative dermatology, 1978-07, Vol.71 (1), p.12-17 |
| issn | 0022-202X 1523-1747 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Blood Cells, Cultured Chemical Phenomena Chemistry Connective Tissue - metabolism Fibroblasts - metabolism Graves Disease - blood Humans Hyaluronic Acid - biosynthesis Leg Dermatoses - blood Molecular Conformation Myxedema - blood Skin - ultrastructure Stimulation, Chemical |
| title | Stimulation of Fibroblast Biosynthetic Activity by Serum of Patients with Pretibial Myxedema |
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