Hippurate synthesis and ammonia metabolism in isolated hepatocytes

The incorporation of benzoate into hippurate by isolated hepatocytes was limited by the availability of glycine. This limitation was unaffected by the addition of ammonia. When isolated hepatocytes were incubated in a solution containing urease, benzoate was completely ineffective in combating ammon...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 1982-04, Vol.105 (4), p.1341-1346
Hauptverfasser:	Wendler, Philip A., Tremblay, George C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonia - metabolism Animals Benzoates - pharmacology Glycine - metabolism Glycine - pharmacology Hippurates - metabolism In Vitro Techniques Kinetics Liver - drug effects Liver - metabolism Male Rats Rats, Inbred Strains
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	1346
container_issue	4
container_start_page	1341
container_title	Biochemical and biophysical research communications
container_volume	105
creator	Wendler, Philip A. Tremblay, George C.
description	The incorporation of benzoate into hippurate by isolated hepatocytes was limited by the availability of glycine. This limitation was unaffected by the addition of ammonia. When isolated hepatocytes were incubated in a solution containing urease, benzoate was completely ineffective in combating ammonia accumulation, while a mixture of α-keto analogues of several amino acids reduced ammonia accumulation by 50%. These findings do not support the use of benzoate to combat hyperammonemia, but they do support the current use of α-keto analogues of amino acids for that purpose.
doi_str_mv	10.1016/0006-291X(82)90934-2
format	Article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74139430</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0006291X82909342</els_id><sourcerecordid>74139430</sourcerecordid><originalsourceid>FETCH-LOGICAL-c357t-ee141e1277415c872fb8a9b0d32f4631624a9ae350f0310a4f0898864f696f1a3</originalsourceid><addsrcrecordid>eNp9kMFKw0AQhhdRaq2-gUJOoofozO42yV4ELWqFghcFb8tmM6ErSTZmE6Fvb2qLR09zmO__h_kYO0e4QcDkFgCSmCv8uMr4tQIlZMwP2BRBQcwR5CGb_iHH7CSETwBEmagJm6QIQs2zKXtYurYdOtNTFDZNv6bgQmSaIjJ17Rtnopp6k_vKhTpyTeSCr0a2iNbUmt7bTU_hlB2Vpgp0tp8z9v70-LZYxqvX55fF_Sq2Yp72MRFKJORpKnFus5SXeWZUDoXgpUwEJlwaZUjMoQSBYGQJmcqyRJaJSko0YsYud71t578GCr2uXbBUVaYhPwQ99golBYyg3IG28yF0VOq2c7XpNhpBb9XprRe99aIzrn_VaT7GLvb9Q15T8Rfauxr3d7s9jU9-O-p0sI4aS4XryPa68O7_Az9UKX0X</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74139430</pqid></control><display><type>article</type><title>Hippurate synthesis and ammonia metabolism in isolated hepatocytes</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Wendler, Philip A. ; Tremblay, George C.</creator><creatorcontrib>Wendler, Philip A. ; Tremblay, George C.</creatorcontrib><description>The incorporation of benzoate into hippurate by isolated hepatocytes was limited by the availability of glycine. This limitation was unaffected by the addition of ammonia. When isolated hepatocytes were incubated in a solution containing urease, benzoate was completely ineffective in combating ammonia accumulation, while a mixture of α-keto analogues of several amino acids reduced ammonia accumulation by 50%. These findings do not support the use of benzoate to combat hyperammonemia, but they do support the current use of α-keto analogues of amino acids for that purpose.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(82)90934-2</identifier><identifier>PMID: 7103958</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Ammonia - metabolism ; Animals ; Benzoates - pharmacology ; Glycine - metabolism ; Glycine - pharmacology ; Hippurates - metabolism ; In Vitro Techniques ; Kinetics ; Liver - drug effects ; Liver - metabolism ; Male ; Rats ; Rats, Inbred Strains</subject><ispartof>Biochemical and biophysical research communications, 1982-04, Vol.105 (4), p.1341-1346</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-ee141e1277415c872fb8a9b0d32f4631624a9ae350f0310a4f0898864f696f1a3</citedby><cites>FETCH-LOGICAL-c357t-ee141e1277415c872fb8a9b0d32f4631624a9ae350f0310a4f0898864f696f1a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(82)90934-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7103958$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wendler, Philip A.</creatorcontrib><creatorcontrib>Tremblay, George C.</creatorcontrib><title>Hippurate synthesis and ammonia metabolism in isolated hepatocytes</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The incorporation of benzoate into hippurate by isolated hepatocytes was limited by the availability of glycine. This limitation was unaffected by the addition of ammonia. When isolated hepatocytes were incubated in a solution containing urease, benzoate was completely ineffective in combating ammonia accumulation, while a mixture of α-keto analogues of several amino acids reduced ammonia accumulation by 50%. These findings do not support the use of benzoate to combat hyperammonemia, but they do support the current use of α-keto analogues of amino acids for that purpose.</description><subject>Ammonia - metabolism</subject><subject>Animals</subject><subject>Benzoates - pharmacology</subject><subject>Glycine - metabolism</subject><subject>Glycine - pharmacology</subject><subject>Hippurates - metabolism</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFKw0AQhhdRaq2-gUJOoofozO42yV4ELWqFghcFb8tmM6ErSTZmE6Fvb2qLR09zmO__h_kYO0e4QcDkFgCSmCv8uMr4tQIlZMwP2BRBQcwR5CGb_iHH7CSETwBEmagJm6QIQs2zKXtYurYdOtNTFDZNv6bgQmSaIjJ17Rtnopp6k_vKhTpyTeSCr0a2iNbUmt7bTU_hlB2Vpgp0tp8z9v70-LZYxqvX55fF_Sq2Yp72MRFKJORpKnFus5SXeWZUDoXgpUwEJlwaZUjMoQSBYGQJmcqyRJaJSko0YsYud71t578GCr2uXbBUVaYhPwQ99golBYyg3IG28yF0VOq2c7XpNhpBb9XprRe99aIzrn_VaT7GLvb9Q15T8Rfauxr3d7s9jU9-O-p0sI4aS4XryPa68O7_Az9UKX0X</recordid><startdate>19820429</startdate><enddate>19820429</enddate><creator>Wendler, Philip A.</creator><creator>Tremblay, George C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19820429</creationdate><title>Hippurate synthesis and ammonia metabolism in isolated hepatocytes</title><author>Wendler, Philip A. ; Tremblay, George C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-ee141e1277415c872fb8a9b0d32f4631624a9ae350f0310a4f0898864f696f1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Ammonia - metabolism</topic><topic>Animals</topic><topic>Benzoates - pharmacology</topic><topic>Glycine - metabolism</topic><topic>Glycine - pharmacology</topic><topic>Hippurates - metabolism</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wendler, Philip A.</creatorcontrib><creatorcontrib>Tremblay, George C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wendler, Philip A.</au><au>Tremblay, George C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hippurate synthesis and ammonia metabolism in isolated hepatocytes</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1982-04-29</date><risdate>1982</risdate><volume>105</volume><issue>4</issue><spage>1341</spage><epage>1346</epage><pages>1341-1346</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The incorporation of benzoate into hippurate by isolated hepatocytes was limited by the availability of glycine. This limitation was unaffected by the addition of ammonia. When isolated hepatocytes were incubated in a solution containing urease, benzoate was completely ineffective in combating ammonia accumulation, while a mixture of α-keto analogues of several amino acids reduced ammonia accumulation by 50%. These findings do not support the use of benzoate to combat hyperammonemia, but they do support the current use of α-keto analogues of amino acids for that purpose.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7103958</pmid><doi>10.1016/0006-291X(82)90934-2</doi><tpages>6</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0006-291X
ispartof	Biochemical and biophysical research communications, 1982-04, Vol.105 (4), p.1341-1346
issn	0006-291X 1090-2104
language	eng
recordid	cdi_proquest_miscellaneous_74139430
source	MEDLINE; Elsevier ScienceDirect Journals
subjects	Ammonia - metabolism Animals Benzoates - pharmacology Glycine - metabolism Glycine - pharmacology Hippurates - metabolism In Vitro Techniques Kinetics Liver - drug effects Liver - metabolism Male Rats Rats, Inbred Strains
title	Hippurate synthesis and ammonia metabolism in isolated hepatocytes
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T01%3A49%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hippurate%20synthesis%20and%20ammonia%20metabolism%20in%20isolated%20hepatocytes&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Wendler,%20Philip%20A.&rft.date=1982-04-29&rft.volume=105&rft.issue=4&rft.spage=1341&rft.epage=1346&rft.pages=1341-1346&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/0006-291X(82)90934-2&rft_dat=%3Cproquest_cross%3E74139430%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=74139430&rft_id=info:pmid/7103958&rft_els_id=0006291X82909342&rfr_iscdi=true