Study on calcium transport by sarcoplasmic reticulum vesicles using fluorescence probes [rabbit]
Fluorescence changes of l-anilino-8-naphthalenesulfonic acid and 3,3′-dipropyl-2,2′-thiadi-carbocyanine during Ca transport by sarcoplasmic reticulum vesicles were studied. The fluorescence of both probes is enhanced corresponding to the rapid initial Ca uptake. The enhancement could be interpreted...
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| Veröffentlicht in: | Journal of biochemistry (Tokyo) 1978-01, Vol.84 (4), p.787-794 |
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| container_end_page | 794 |
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| container_issue | 4 |
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| container_title | Journal of biochemistry (Tokyo) |
| container_volume | 84 |
| creator | UENO, Takashi SEKINE, Takamitsu |
| description | Fluorescence changes of l-anilino-8-naphthalenesulfonic acid and 3,3′-dipropyl-2,2′-thiadi-carbocyanine during Ca transport by sarcoplasmic reticulum vesicles were studied. The fluorescence of both probes is enhanced corresponding to the rapid initial Ca uptake. The enhancement could be interpreted in terms of increased binding of the former (anionic) dye and decreased binding of the latter (cationic) dye to the vesicles, suggesting a change in the surface charge of the membranes associated with Ca transport. Under limited conditions where Na, K, and Mg were not added exogenously, Ca could be transported without concomitant counter-transport of these cations. |
| doi_str_mv | 10.1093/oxfordjournals.jbchem.a132190 |
| format | Article |
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School of Medicine ; Ministerstvo Sel' skogo Khozyajstva SSSR, Moscow</creatorcontrib><description>Fluorescence changes of l-anilino-8-naphthalenesulfonic acid and 3,3′-dipropyl-2,2′-thiadi-carbocyanine during Ca transport by sarcoplasmic reticulum vesicles were studied. The fluorescence of both probes is enhanced corresponding to the rapid initial Ca uptake. The enhancement could be interpreted in terms of increased binding of the former (anionic) dye and decreased binding of the latter (cationic) dye to the vesicles, suggesting a change in the surface charge of the membranes associated with Ca transport. Under limited conditions where Na, K, and Mg were not added exogenously, Ca could be transported without concomitant counter-transport of these cations.</description><identifier>ISSN: 0021-924X</identifier><identifier>EISSN: 1756-2651</identifier><identifier>DOI: 10.1093/oxfordjournals.jbchem.a132190</identifier><identifier>PMID: 711700</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adenosine Triphosphate - metabolism ; Anilino Naphthalenesulfonates ; Animals ; Biological Transport, Active ; Calcium - metabolism ; Carbocyanines ; Kinetics ; Muscles - metabolism ; Rabbits ; Sarcoplasmic Reticulum - metabolism ; Spectrometry, Fluorescence</subject><ispartof>Journal of biochemistry (Tokyo), 1978-01, Vol.84 (4), p.787-794</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/711700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>UENO, Takashi</creatorcontrib><creatorcontrib>SEKINE, Takamitsu</creatorcontrib><creatorcontrib>Juntendo Univ., Tokyo (Japan). School of Medicine</creatorcontrib><creatorcontrib>Ministerstvo Sel' skogo Khozyajstva SSSR, Moscow</creatorcontrib><title>Study on calcium transport by sarcoplasmic reticulum vesicles using fluorescence probes [rabbit]</title><title>Journal of biochemistry (Tokyo)</title><addtitle>J Biochem</addtitle><description>Fluorescence changes of l-anilino-8-naphthalenesulfonic acid and 3,3′-dipropyl-2,2′-thiadi-carbocyanine during Ca transport by sarcoplasmic reticulum vesicles were studied. The fluorescence of both probes is enhanced corresponding to the rapid initial Ca uptake. The enhancement could be interpreted in terms of increased binding of the former (anionic) dye and decreased binding of the latter (cationic) dye to the vesicles, suggesting a change in the surface charge of the membranes associated with Ca transport. Under limited conditions where Na, K, and Mg were not added exogenously, Ca could be transported without concomitant counter-transport of these cations.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Anilino Naphthalenesulfonates</subject><subject>Animals</subject><subject>Biological Transport, Active</subject><subject>Calcium - metabolism</subject><subject>Carbocyanines</subject><subject>Kinetics</subject><subject>Muscles - metabolism</subject><subject>Rabbits</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><subject>Spectrometry, Fluorescence</subject><issn>0021-924X</issn><issn>1756-2651</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kG9vFCEQh4lR27P6DZqGN_puT9hh4XjZNPaPbaKNmjQ2DQUWKufucsLS9L69mGt8NTN5nsz8Mgi9p2RJiYSP8cnH1K9jSZMe8nJt7C83LjWFlkryAi2o6HjT8o6-RAtCWtrIlt3sozc5r_-NLcAeei0oFYQs0P23ufRbHCds9WBDGfGc9JQ3Mc3YbHHWycbNoPMYLE5uDrYM1Xl0OdjBZVxymB6wH0pMLls3WYc3KZpKbpM2Jsx3b9ErX2O6d8_1AP04_fT95Ly5-nJ2cXJ81XjgbG60472xQDsJhgMRPbDOUAZ974Wt3WrVd1qsnOUrD62X4JyTTApuwduOWThAH3Z76_0_xeVZjaEmGgY9uViyEowCF1JU8fBZLGZ0vdqkMOq0VbuPVNzscMize_pPdfqtuADRqfObn0peX56xU3Kprqt_tPO9jko_pJDV569UCkkYbSXn8BcAAoQh</recordid><startdate>19780101</startdate><enddate>19780101</enddate><creator>UENO, Takashi</creator><creator>SEKINE, Takamitsu</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19780101</creationdate><title>Study on calcium transport by sarcoplasmic reticulum vesicles using fluorescence probes [rabbit]</title><author>UENO, Takashi ; SEKINE, Takamitsu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f364t-ae6dbc31593b6307d345b143ddf7c5b188d5a78ec68f32f93eee94976c3fc54c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Anilino Naphthalenesulfonates</topic><topic>Animals</topic><topic>Biological Transport, Active</topic><topic>Calcium - metabolism</topic><topic>Carbocyanines</topic><topic>Kinetics</topic><topic>Muscles - metabolism</topic><topic>Rabbits</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><topic>Spectrometry, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>UENO, Takashi</creatorcontrib><creatorcontrib>SEKINE, Takamitsu</creatorcontrib><creatorcontrib>Juntendo Univ., Tokyo (Japan). School of Medicine</creatorcontrib><creatorcontrib>Ministerstvo Sel' skogo Khozyajstva SSSR, Moscow</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>UENO, Takashi</au><au>SEKINE, Takamitsu</au><aucorp>Juntendo Univ., Tokyo (Japan). School of Medicine</aucorp><aucorp>Ministerstvo Sel' skogo Khozyajstva SSSR, Moscow</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study on calcium transport by sarcoplasmic reticulum vesicles using fluorescence probes [rabbit]</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>1978-01-01</date><risdate>1978</risdate><volume>84</volume><issue>4</issue><spage>787</spage><epage>794</epage><pages>787-794</pages><issn>0021-924X</issn><eissn>1756-2651</eissn><abstract>Fluorescence changes of l-anilino-8-naphthalenesulfonic acid and 3,3′-dipropyl-2,2′-thiadi-carbocyanine during Ca transport by sarcoplasmic reticulum vesicles were studied. The fluorescence of both probes is enhanced corresponding to the rapid initial Ca uptake. The enhancement could be interpreted in terms of increased binding of the former (anionic) dye and decreased binding of the latter (cationic) dye to the vesicles, suggesting a change in the surface charge of the membranes associated with Ca transport. Under limited conditions where Na, K, and Mg were not added exogenously, Ca could be transported without concomitant counter-transport of these cations.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>711700</pmid><doi>10.1093/oxfordjournals.jbchem.a132190</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0021-924X |
| ispartof | Journal of biochemistry (Tokyo), 1978-01, Vol.84 (4), p.787-794 |
| issn | 0021-924X 1756-2651 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74136797 |
| source | J-STAGE Free; MEDLINE; Oxford University Press Journals Digital Archive Legacy; Free Full-Text Journals in Chemistry |
| subjects | Adenosine Triphosphate - metabolism Anilino Naphthalenesulfonates Animals Biological Transport, Active Calcium - metabolism Carbocyanines Kinetics Muscles - metabolism Rabbits Sarcoplasmic Reticulum - metabolism Spectrometry, Fluorescence |
| title | Study on calcium transport by sarcoplasmic reticulum vesicles using fluorescence probes [rabbit] |
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