Quantitative determination of free thiol and disulphide groups by a fluorescent maleimide procedure

To determine the quantitative reliability of thiol histofluorometry, the distribution of protein thiol and disulphide groups was reinvestigated in normal human epidermis, labelled with DACM-3 after cryocutting. Microscopical observations roughly confirmed, that living keratinocytes and inner stratum...

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Veröffentlicht in:	Acta histochemica 1982, Vol.70 (1), p.62-68
Hauptverfasser:	Broekaert, D, Coucke, P, Nsabumukunzi, S, Reyniers, P, van Oostveldt, P, Kluyskens, P, Gillis, E
Format:	Artikel
Sprache:	eng
Schlagworte:	Disulfides - analysis Flow Cytometry Humans Maleimides Microscopy, Fluorescence Skin - cytology Staining and Labeling Sulfhydryl Compounds - analysis Sulfhydryl Reagents
Online-Zugang:	Volltext
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