Production and titration of African swine fever virus in porcine alveolar macrophages
The broncho-alveolar lavage of a pig (20–40 kg) contains about 1.6 × 10 9 alveolar cells, half of which were macrophages. The number of cells in the lavage of bacille Calmette Guerin (BCG)-treated pigs increased about 4-fold. Both African swine fever virus-infected porcine alveolar macrophages and b...
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| Veröffentlicht in: | Journal of virological methods 1982, Vol.3 (6), p.303-310 |
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| container_title | Journal of virological methods |
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| creator | Carrascosa, Angel L. Santarén, Juan F. Viñuela, Eladio |
| description | The broncho-alveolar lavage of a pig (20–40 kg) contains about 1.6 × 10
9 alveolar cells, half of which were macrophages. The number of cells in the lavage of bacille Calmette Guerin (BCG)-treated pigs increased about 4-fold. Both African swine fever virus-infected porcine alveolar macrophages and blood monocytes produced about 1000 hemadsorption units/cell, a value 10-fold larger than that obtained in virus-infected Vero cells. Porcine alveolar cells could be stored frozen and, after thawing, they could be infected with African swine fever virus, producing the same amount of virus as the unfrozen cells. With the number of alveolar macrophages obtained from a single pig it is possible to titer about 3000 virus samples with the same stock of alveolar macrophages. |
| doi_str_mv | 10.1016/0166-0934(82)90034-9 |
| format | Article |
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9 alveolar cells, half of which were macrophages. The number of cells in the lavage of bacille Calmette Guerin (BCG)-treated pigs increased about 4-fold. Both African swine fever virus-infected porcine alveolar macrophages and blood monocytes produced about 1000 hemadsorption units/cell, a value 10-fold larger than that obtained in virus-infected Vero cells. Porcine alveolar cells could be stored frozen and, after thawing, they could be infected with African swine fever virus, producing the same amount of virus as the unfrozen cells. With the number of alveolar macrophages obtained from a single pig it is possible to titer about 3000 virus samples with the same stock of alveolar macrophages.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/0166-0934(82)90034-9</identifier><identifier>PMID: 7085838</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>African swine fever virus ; African Swine Fever Virus - growth & development ; alveolar cells ; Animals ; BCG Vaccine - pharmacology ; Cells, Cultured ; Cercopithecus aethiops ; Hemadsorption ; Iridoviridae - growth & development ; Kidney ; Macrophages ; Monocytes ; porcine alveolar macrophages ; Pulmonary Alveoli ; Swine ; Virus Cultivation</subject><ispartof>Journal of virological methods, 1982, Vol.3 (6), p.303-310</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-80d92ab649fae1f4fd08c69c1782cf224334e9797ce285087eedbff094fa76ab3</citedby><cites>FETCH-LOGICAL-c388t-80d92ab649fae1f4fd08c69c1782cf224334e9797ce285087eedbff094fa76ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0166-0934(82)90034-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,4012,27906,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7085838$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carrascosa, Angel L.</creatorcontrib><creatorcontrib>Santarén, Juan F.</creatorcontrib><creatorcontrib>Viñuela, Eladio</creatorcontrib><title>Production and titration of African swine fever virus in porcine alveolar macrophages</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>The broncho-alveolar lavage of a pig (20–40 kg) contains about 1.6 × 10
9 alveolar cells, half of which were macrophages. The number of cells in the lavage of bacille Calmette Guerin (BCG)-treated pigs increased about 4-fold. Both African swine fever virus-infected porcine alveolar macrophages and blood monocytes produced about 1000 hemadsorption units/cell, a value 10-fold larger than that obtained in virus-infected Vero cells. Porcine alveolar cells could be stored frozen and, after thawing, they could be infected with African swine fever virus, producing the same amount of virus as the unfrozen cells. With the number of alveolar macrophages obtained from a single pig it is possible to titer about 3000 virus samples with the same stock of alveolar macrophages.</description><subject>African swine fever virus</subject><subject>African Swine Fever Virus - growth & development</subject><subject>alveolar cells</subject><subject>Animals</subject><subject>BCG Vaccine - pharmacology</subject><subject>Cells, Cultured</subject><subject>Cercopithecus aethiops</subject><subject>Hemadsorption</subject><subject>Iridoviridae - growth & development</subject><subject>Kidney</subject><subject>Macrophages</subject><subject>Monocytes</subject><subject>porcine alveolar macrophages</subject><subject>Pulmonary Alveoli</subject><subject>Swine</subject><subject>Virus Cultivation</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotX78A4WcRA-r-eomuQil-AWCHuw5pNmJRrabmuyu-O_d2uJRD8MwM--8wzwInVBySQktr4YoC6K5OFfsQhPCRaF30JgqqYe2Erto_CvZRwc5vxNCJpLzERpJoiaKqzGaP6dYda4NscG2qXAb2mR_qujx1KfgbIPzZ2gAe-gh4T6kLuPQ4FVMbt22dQ-xtgkvrUtx9WZfIR-hPW_rDMfbfIjmtzcvs_vi8enuYTZ9LBxXqi0UqTSzi1Job4F64SuiXKkdlYo5z5jgXICWWjpgakKUBKgW3hMtvJWlXfBDdLbxXaX40UFuzTJkB3VtG4hdNlJQpmWp_hXSidADKzYIxUY4_JJzAm9WKSxt-jKUmDV2s2Zq1kyNYuYHu9HD2unWv1ssofpd2nIe5tebOQw0-gDJZBegcVCFBK41VQx_H_gGXb2SVA</recordid><startdate>1982</startdate><enddate>1982</enddate><creator>Carrascosa, Angel L.</creator><creator>Santarén, Juan F.</creator><creator>Viñuela, Eladio</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1982</creationdate><title>Production and titration of African swine fever virus in porcine alveolar macrophages</title><author>Carrascosa, Angel L. ; Santarén, Juan F. ; Viñuela, Eladio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-80d92ab649fae1f4fd08c69c1782cf224334e9797ce285087eedbff094fa76ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>African swine fever virus</topic><topic>African Swine Fever Virus - growth & development</topic><topic>alveolar cells</topic><topic>Animals</topic><topic>BCG Vaccine - pharmacology</topic><topic>Cells, Cultured</topic><topic>Cercopithecus aethiops</topic><topic>Hemadsorption</topic><topic>Iridoviridae - growth & development</topic><topic>Kidney</topic><topic>Macrophages</topic><topic>Monocytes</topic><topic>porcine alveolar macrophages</topic><topic>Pulmonary Alveoli</topic><topic>Swine</topic><topic>Virus Cultivation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carrascosa, Angel L.</creatorcontrib><creatorcontrib>Santarén, Juan F.</creatorcontrib><creatorcontrib>Viñuela, Eladio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carrascosa, Angel L.</au><au>Santarén, Juan F.</au><au>Viñuela, Eladio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production and titration of African swine fever virus in porcine alveolar macrophages</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>1982</date><risdate>1982</risdate><volume>3</volume><issue>6</issue><spage>303</spage><epage>310</epage><pages>303-310</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><abstract>The broncho-alveolar lavage of a pig (20–40 kg) contains about 1.6 × 10
9 alveolar cells, half of which were macrophages. The number of cells in the lavage of bacille Calmette Guerin (BCG)-treated pigs increased about 4-fold. Both African swine fever virus-infected porcine alveolar macrophages and blood monocytes produced about 1000 hemadsorption units/cell, a value 10-fold larger than that obtained in virus-infected Vero cells. Porcine alveolar cells could be stored frozen and, after thawing, they could be infected with African swine fever virus, producing the same amount of virus as the unfrozen cells. With the number of alveolar macrophages obtained from a single pig it is possible to titer about 3000 virus samples with the same stock of alveolar macrophages.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>7085838</pmid><doi>10.1016/0166-0934(82)90034-9</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 1982, Vol.3 (6), p.303-310 |
| issn | 0166-0934 1879-0984 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | African swine fever virus African Swine Fever Virus - growth & development alveolar cells Animals BCG Vaccine - pharmacology Cells, Cultured Cercopithecus aethiops Hemadsorption Iridoviridae - growth & development Kidney Macrophages Monocytes porcine alveolar macrophages Pulmonary Alveoli Swine Virus Cultivation |
| title | Production and titration of African swine fever virus in porcine alveolar macrophages |
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