[35] N-asparagine-linked oligosaccharides: Biosynthesis of the lipid-linked oligosaccharides

This chapter presents the procedures utilized by laboratories to radiolabel the dolichol-linked oligosaccharides and study their biosynthesis. Two general approaches are adopted. The chapter primarily summarizes the in vivo studies, where pulse-labeling is used to identify intermediates and determin...

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Veröffentlicht in:	Methods in Enzymology 1982, Vol.83, p.399-408
Hauptverfasser:	Rosner, Marsha R., Catherine Hubbard, S., Ivatt, Raymond J., Robbins, Phillips W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Asparagine - metabolism Cell-Free System Cells, Cultured Chick Embryo Cricetinae Kinetics Microsomes - metabolism Oligosaccharides - biosynthesis Polyisoprenyl Phosphate Oligosaccharides - biosynthesis Polyisoprenyl Phosphate Sugars - biosynthesis
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