Purification of an almond emulsin fucosidase on Cibacron blue-sepharose and demonstration of its activity toward fucose-containing glycoproteins

The almond emulsin fucosidase that specifically hydrolyzes fucose in alpha (1-3) linkage to N-acetylglucosamine has been purified 1250-fold. The purification procedure includes ion exchange chromatography on sulfopropyl-Sephadex C-25, gel filtration on Sephacryl S-200, and affinity chromatography on...

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Veröffentlicht in:	The Journal of biological chemistry 1982-07, Vol.257 (14), p.8205-8210
Hauptverfasser:	Imber, M J, Glasgow, L R, Pizzo, S V
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-L-Fucosidase - isolation & purification alpha-L-Fucosidase - metabolism Glycopeptides Glycoside Hydrolases - isolation & purification Glycoside Hydrolases - metabolism Immunoglobulin G Kinetics Molecular Weight Plants - enzymology Substrate Specificity
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