Automatic collection and processing of data from the ultracentrifuge using a programmable desk calculator
Previous investigators [Trautman, R., Spragg, S. P., and Halsall, H. B. (1969) Anal. Biochem. 28, 396–415] have published a detailed protocol for the analysis of sedimentation velocity measurements which is adaptable to data generated by an ultraviolet scanning system. The advent of programmable des...
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| Veröffentlicht in: | Analytical biochemistry 1978-07, Vol.87 (2), p.477-495 |
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| container_title | Analytical biochemistry |
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| creator | Inners, Daniel Tindall, Stephen H. Aune, Kirk C. |
| description | Previous investigators [Trautman, R., Spragg, S. P., and Halsall, H. B. (1969)
Anal. Biochem.
28, 396–415] have published a detailed protocol for the analysis of sedimentation velocity measurements which is adaptable to data generated by an ultraviolet scanning system. The advent of programmable desk calculators capable of sampling the output of digital measuring devices has made it possible to develop inexpensive and highly convenient systems for collecting and processing scanner data. Basing our approach on the referenced protocol, we have developed algorithms for dealing with real data, that is, data characterized by a relatively high level of noise. The techniques are applieable to both sedimentation equilibrium and sedimentation velocity measurements using the seanning system and multicell rotors. With known concentration dependence, valid estimates of weight-average sedimentation coefficients, diffusion coefficients, and heterogeneity parameters have been obtained for both simulated and actual sedimenting anddiffusing macromolecular solutes. We find, however, that concentration dependence derived internally from a single sedimentation velocity measurement is unreliable. |
| doi_str_mv | 10.1016/0003-2697(78)90698-X |
| format | Article |
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Anal. Biochem.
28, 396–415] have published a detailed protocol for the analysis of sedimentation velocity measurements which is adaptable to data generated by an ultraviolet scanning system. The advent of programmable desk calculators capable of sampling the output of digital measuring devices has made it possible to develop inexpensive and highly convenient systems for collecting and processing scanner data. Basing our approach on the referenced protocol, we have developed algorithms for dealing with real data, that is, data characterized by a relatively high level of noise. The techniques are applieable to both sedimentation equilibrium and sedimentation velocity measurements using the seanning system and multicell rotors. With known concentration dependence, valid estimates of weight-average sedimentation coefficients, diffusion coefficients, and heterogeneity parameters have been obtained for both simulated and actual sedimenting anddiffusing macromolecular solutes. We find, however, that concentration dependence derived internally from a single sedimentation velocity measurement is unreliable.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(78)90698-X</identifier><identifier>PMID: 356670</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Autoanalysis ; Cattle ; Chymotrypsinogen ; Escherichia coli ; Molecular Weight ; Pancreas - enzymology ; Ribosomes - ultrastructure ; Ultracentrifugation - methods</subject><ispartof>Analytical biochemistry, 1978-07, Vol.87 (2), p.477-495</ispartof><rights>1978</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c271t-e2e0dc9655c776f9889c2a8d340520d5ad7d7fa3e1b21ab00c9d8e064479b1cf3</citedby><cites>FETCH-LOGICAL-c271t-e2e0dc9655c776f9889c2a8d340520d5ad7d7fa3e1b21ab00c9d8e064479b1cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/000326977890698X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/356670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inners, Daniel</creatorcontrib><creatorcontrib>Tindall, Stephen H.</creatorcontrib><creatorcontrib>Aune, Kirk C.</creatorcontrib><title>Automatic collection and processing of data from the ultracentrifuge using a programmable desk calculator</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Previous investigators [Trautman, R., Spragg, S. P., and Halsall, H. B. (1969)
Anal. Biochem.
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Anal. Biochem.
28, 396–415] have published a detailed protocol for the analysis of sedimentation velocity measurements which is adaptable to data generated by an ultraviolet scanning system. The advent of programmable desk calculators capable of sampling the output of digital measuring devices has made it possible to develop inexpensive and highly convenient systems for collecting and processing scanner data. Basing our approach on the referenced protocol, we have developed algorithms for dealing with real data, that is, data characterized by a relatively high level of noise. The techniques are applieable to both sedimentation equilibrium and sedimentation velocity measurements using the seanning system and multicell rotors. With known concentration dependence, valid estimates of weight-average sedimentation coefficients, diffusion coefficients, and heterogeneity parameters have been obtained for both simulated and actual sedimenting anddiffusing macromolecular solutes. We find, however, that concentration dependence derived internally from a single sedimentation velocity measurement is unreliable.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>356670</pmid><doi>10.1016/0003-2697(78)90698-X</doi><tpages>19</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1978-07, Vol.87 (2), p.477-495 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Autoanalysis Cattle Chymotrypsinogen Escherichia coli Molecular Weight Pancreas - enzymology Ribosomes - ultrastructure Ultracentrifugation - methods |
| title | Automatic collection and processing of data from the ultracentrifuge using a programmable desk calculator |
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