Inhibition of lymphoproliferation by dipyridamole
Dipyridamole (DP, Persantin) was examined for its effects on the proliferation of mitogen-stimulated murine splenocytes and L1210 leukemia cells. In keeping with its reported activity as an inhibitor of nucleoside transport, DP inhibited incorporation by lymphoid cells of labeled thymidine and uridi...
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| Veröffentlicht in: | Biochemical pharmacology 1982-04, Vol.31 (7), p.1381-1386 |
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| container_title | Biochemical pharmacology |
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| creator | Farmer, Jeffrey L. Prager, Morton D. |
| description | Dipyridamole (DP, Persantin) was examined for its effects on the proliferation of mitogen-stimulated murine splenocytes and L1210 leukemia cells. In keeping with its reported activity as an inhibitor of nucleoside transport, DP inhibited incorporation by lymphoid cells of labeled thymidine and uridine into macromolecules. That this inhibition resulted from activities in addition to suppression of nucleoside transport was verified by measured decreases of cellular DNA and viable cell numbers. In addition, protein synthesis was also decreased as indicated by labeled valine incorporation and total protein content of the cells. The rapid accumulation of cAMP in phytohemagglutinin-stimulated splenocytes in the presence of DP may provide an explanation for the anti-proliferative effect of DP on lymphoid cells. |
| doi_str_mv | 10.1016/0006-2952(82)90032-6 |
| format | Article |
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In keeping with its reported activity as an inhibitor of nucleoside transport, DP inhibited incorporation by lymphoid cells of labeled thymidine and uridine into macromolecules. That this inhibition resulted from activities in addition to suppression of nucleoside transport was verified by measured decreases of cellular DNA and viable cell numbers. In addition, protein synthesis was also decreased as indicated by labeled valine incorporation and total protein content of the cells. The rapid accumulation of cAMP in phytohemagglutinin-stimulated splenocytes in the presence of DP may provide an explanation for the anti-proliferative effect of DP on lymphoid cells.</description><identifier>ISSN: 0006-2952</identifier><identifier>EISSN: 1873-2968</identifier><identifier>DOI: 10.1016/0006-2952(82)90032-6</identifier><identifier>PMID: 7092928</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Animals ; Biological Transport - drug effects ; Cell Division - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Dipyridamole - pharmacology ; In Vitro Techniques ; Leukemia L1210 - pathology ; Lymphocytes - cytology ; Lymphocytes - drug effects ; Lymphocytes - metabolism ; Macromolecular Substances ; Male ; Mice ; Mice, Inbred A ; Spleen - cytology</subject><ispartof>Biochemical pharmacology, 1982-04, Vol.31 (7), p.1381-1386</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-544f94b49a1a9944dbc4731ccf7783b11c2a857ecb733a344487f0ced63ea8ea3</citedby><cites>FETCH-LOGICAL-c357t-544f94b49a1a9944dbc4731ccf7783b11c2a857ecb733a344487f0ced63ea8ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-2952(82)90032-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7092928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Farmer, Jeffrey L.</creatorcontrib><creatorcontrib>Prager, Morton D.</creatorcontrib><title>Inhibition of lymphoproliferation by dipyridamole</title><title>Biochemical pharmacology</title><addtitle>Biochem Pharmacol</addtitle><description>Dipyridamole (DP, Persantin) was examined for its effects on the proliferation of mitogen-stimulated murine splenocytes and L1210 leukemia cells. In keeping with its reported activity as an inhibitor of nucleoside transport, DP inhibited incorporation by lymphoid cells of labeled thymidine and uridine into macromolecules. That this inhibition resulted from activities in addition to suppression of nucleoside transport was verified by measured decreases of cellular DNA and viable cell numbers. In addition, protein synthesis was also decreased as indicated by labeled valine incorporation and total protein content of the cells. The rapid accumulation of cAMP in phytohemagglutinin-stimulated splenocytes in the presence of DP may provide an explanation for the anti-proliferative effect of DP on lymphoid cells.</description><subject>Animals</subject><subject>Biological Transport - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Dipyridamole - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Leukemia L1210 - pathology</subject><subject>Lymphocytes - cytology</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - metabolism</subject><subject>Macromolecular Substances</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred A</subject><subject>Spleen - cytology</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UEtLw0AQXkSptfoPFHoSPUT3lX1cBCk-CgUvel42mwldSbJxtxXy703a0qOnYeZ7zMyH0DXBDwQT8YgxFhnVOb1T9F5jzGgmTtCUKMmGsVCnaHqknKOLlL7HVgkyQROJNdVUTRFZtmtf-I0P7TxU87pvunXoYqh9BdHuxkU_L33XR1_aJtRwic4qWye4OtQZ-np9-Vy8Z6uPt-XieZU5lstNlnNeaV5wbYnVmvOycFwy4lwlpWIFIY5alUtwhWTMMs65khV2UAoGVoFlM3S79x2u-dlC2pjGJwd1bVsI22QkJ1jkQg9Evie6GFKKUJku-sbG3hBsxqTM-LcZYzCKml1SRgyym4P_tmigPIoO0Qz40x6H4clfD9Ek56EdLvQR3MaUwf-_4A8-yXd_</recordid><startdate>19820401</startdate><enddate>19820401</enddate><creator>Farmer, Jeffrey L.</creator><creator>Prager, Morton D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19820401</creationdate><title>Inhibition of lymphoproliferation by dipyridamole</title><author>Farmer, Jeffrey L. ; Prager, Morton D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-544f94b49a1a9944dbc4731ccf7783b11c2a857ecb733a344487f0ced63ea8ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Biological Transport - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Dipyridamole - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Leukemia L1210 - pathology</topic><topic>Lymphocytes - cytology</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - metabolism</topic><topic>Macromolecular Substances</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred A</topic><topic>Spleen - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Farmer, Jeffrey L.</creatorcontrib><creatorcontrib>Prager, Morton D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Farmer, Jeffrey L.</au><au>Prager, Morton D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of lymphoproliferation by dipyridamole</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>1982-04-01</date><risdate>1982</risdate><volume>31</volume><issue>7</issue><spage>1381</spage><epage>1386</epage><pages>1381-1386</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><abstract>Dipyridamole (DP, Persantin) was examined for its effects on the proliferation of mitogen-stimulated murine splenocytes and L1210 leukemia cells. In keeping with its reported activity as an inhibitor of nucleoside transport, DP inhibited incorporation by lymphoid cells of labeled thymidine and uridine into macromolecules. That this inhibition resulted from activities in addition to suppression of nucleoside transport was verified by measured decreases of cellular DNA and viable cell numbers. In addition, protein synthesis was also decreased as indicated by labeled valine incorporation and total protein content of the cells. The rapid accumulation of cAMP in phytohemagglutinin-stimulated splenocytes in the presence of DP may provide an explanation for the anti-proliferative effect of DP on lymphoid cells.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>7092928</pmid><doi>10.1016/0006-2952(82)90032-6</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Biological Transport - drug effects Cell Division - drug effects Cell Survival - drug effects Cells, Cultured Dipyridamole - pharmacology In Vitro Techniques Leukemia L1210 - pathology Lymphocytes - cytology Lymphocytes - drug effects Lymphocytes - metabolism Macromolecular Substances Male Mice Mice, Inbred A Spleen - cytology |
| title | Inhibition of lymphoproliferation by dipyridamole |
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