Purification of yeast hexokinase isoenzymes using affinity chromatography and chromatofocusing

A new procedure has been devised for the rapid isolation of yeast hexokinase isoenzymes PI and PII, giving specific activities comparable to those obtained after conventional purification. Hexokinases were bound to d-glucosamine, which had been coupled to CH Sepharose 4B using 6-aminohexanoic acid a...

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Veröffentlicht in:	Analytical biochemistry 1982-03, Vol.121 (1), p.181-185
Hauptverfasser:	Kopetzki, E., Entian, K.-D.
Format:	Artikel
Sprache:	eng
Schlagworte:	affinity chromatography Chromatography, Affinity hexokinase Hexokinase - isolation & purification Isoelectric Focusing isoenzymes Isoenzymes - isolation & purification purification Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology
Online-Zugang:	Volltext
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