Distribution and tissue specificity of 4-aminobutyrate-2-oxoglutarate aminotransferase

Distribution and tissue specificity of 4-aminobutyrate-2-oxoglutarate aminotransferase

A rapid and specific method for assaying 4-aminobutyrate-2-oxoglutarate aminotransferase was developed. The method was based on the selectivity of ion exchange resin and the speed of vacuum filtration. With this new method, the aminotransferase activity in various tissues has been determined as foll...

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Veröffentlicht in:Neurochemical research 1978-04, Vol.3 (2), p.207-219
Hauptverfasser: Wu, J Y, Moss, L G, Chude, O
Format: Artikel
Sprache:eng
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4-Aminobutyrate Transaminase - analysis
Animals
Brain - enzymology
Carbon Radioisotopes
Complement Fixation Tests
Immunodiffusion
Isotope Labeling - methods
Kinetics
Mice
Spinal Cord - enzymology
Tissue Distribution
Transaminases - analysis
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Moss, L G
Chude, O
description A rapid and specific method for assaying 4-aminobutyrate-2-oxoglutarate aminotransferase was developed. The method was based on the selectivity of ion exchange resin and the speed of vacuum filtration. With this new method, the aminotransferase activity in various tissues has been determined as follows: brain, 10.2; spinal cord, 11.8; liver, 5.7; kidney, 4.6; heart, 0.5; lung, 0.4 nmol glutamate formed/min/mg. No activity could be detected in muscle preparations. When the aminotransferases were tested with the antibody against the purified 4-aminobutyrate aminotransferase from brain, no difference could be detected among brain, spinal cord, and kidney preparations as judged from the results of immunodiffusion, inhibition of enzyme activity by antibody, and microcomplement fixation. It is concluded that 4-aminobutyrate aminotransferases from various tissues of the mouse are probably identical or closely related.
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The method was based on the selectivity of ion exchange resin and the speed of vacuum filtration. With this new method, the aminotransferase activity in various tissues has been determined as follows: brain, 10.2; spinal cord, 11.8; liver, 5.7; kidney, 4.6; heart, 0.5; lung, 0.4 nmol glutamate formed/min/mg. No activity could be detected in muscle preparations. When the aminotransferases were tested with the antibody against the purified 4-aminobutyrate aminotransferase from brain, no difference could be detected among brain, spinal cord, and kidney preparations as judged from the results of immunodiffusion, inhibition of enzyme activity by antibody, and microcomplement fixation. 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subjects 4-Aminobutyrate Transaminase - analysis
Animals
Brain - enzymology
Carbon Radioisotopes
Complement Fixation Tests
Immunodiffusion
Isotope Labeling - methods
Kinetics
Mice
Spinal Cord - enzymology
Tissue Distribution
Transaminases - analysis
title Distribution and tissue specificity of 4-aminobutyrate-2-oxoglutarate aminotransferase
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