Binding site of a dextran-specific homogeneous IgM: thermodynamic and spectroscopic mapping by dansylated oligosaccharides

The hapten binding properties of the homogeneous mouse IgM secreted by MOPC-104E were investigated. Hapten-association constants were determined either by equilibrium and displacement equilibrium dialysis or by fluorometric titrations of the protein with the fluorescent derivatives of the haptens. F...

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Veröffentlicht in:	Biochemistry (Easton) 1978-05, Vol.17 (11), p.2239-2245
Hauptverfasser:	Schepers, Georg, Blatt, Yoav, Himmelspach, Karl, Pecht, Israel
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites Cell Line Dansyl Compounds Dextrans Haptens Immunoglobulin M Oligosaccharides Protein Binding Spectrophotometry Structure-Activity Relationship Thermodynamics
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