The cyanogen bromide peptides of the apoprotein of low density lipoprotein (apoB): Its molecular weight from a chemical view

After >95% cleavage of the apoprotein (apoB) of the low density lipoproteins with cyanogen bromide, the peptides produced are shown to be extensively aggregated in sodium dodecyl sulfate. Both high temperature and increased concentration (5%) of the detergent are necessary to shift the aggregated...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 1978-04, Vol.81 (3), p.928-935
Hauptverfasser:	Bradley, W.A., Rohde, M.F., Gotto, Antonio M., Jackson, R.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acids - analysis Apolipoproteins - blood Cyanogen Bromide Humans Lipoproteins, LDL - blood Molecular Weight Peptide Fragments - analysis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	935
container_issue	3
container_start_page	928
container_title	Biochemical and biophysical research communications
container_volume	81
creator	Bradley, W.A. Rohde, M.F. Gotto, Antonio M. Jackson, R.L.
description	After >95% cleavage of the apoprotein (apoB) of the low density lipoproteins with cyanogen bromide, the peptides produced are shown to be extensively aggregated in sodium dodecyl sulfate. Both high temperature and increased concentration (5%) of the detergent are necessary to shift the aggregated peptides from high molecular weight (>25,000) to lower molecular weight aggregates as seen on sodium dodecyl sulfate polyacrylamide gel electrophoresis. End group analyses of the cyanogen bromide digestion by automated sequencer techniques indicate the presence of five (5) methionines. With a known methionine content of 16 moles/100,000 g protein, the molecular weight of the apoprotein must be approximately 30,000.
doi_str_mv	10.1016/0006-291X(78)91440-7
format	Article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74045417</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0006291X78914407</els_id><sourcerecordid>74045417</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-e939fe40d11ef249eed3719dd3448ca20f4aef4f65b088995f38ca9ad8091a363</originalsourceid><addsrcrecordid>eNp9kE9P3DAQxa2qtN3SfgMOPiE4pIwT5485IBUELRISF5B6s7z2mDVK4mB7u1qJD4_DInrraaR5v3ma9wg5YPCDAWtOAKApSsH-HLXdsWCcQ9F-IAsGAoqSAf9IFu_IF_I1xkcAxngjPpNPJXQ1hwV5vlsh1Vs1-gcc6TL4wRmkE04pz0i9pSkDavJT8AndOG96v6EGx-jSlvbun3SUsfPjU3qdIh18j3rdq0A36B5WidpsTRXVKxycVj3963DzjexZ1Uf8_jb3yf3V5d3F7-Lm9tf1xc-bQld1kwoUlbDIwTCGtuQC0VQtE8ZUnHdalWC5QsttUy-h64SobZXXQpkOBFNVU-2Tw51v_vRpjTHJwUWNfa9G9OsoWw685qzNIN-BOvgYA1o5BTeosJUM5Ny5nAuVc6Gy7eRr53I-O3jzXy8HNO9Hu5KzfLaTMWfMuYOM2uGo0biAOknj3f_9XwA_bJI7</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74045417</pqid></control><display><type>article</type><title>The cyanogen bromide peptides of the apoprotein of low density lipoprotein (apoB): Its molecular weight from a chemical view</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Bradley, W.A. ; Rohde, M.F. ; Gotto, Antonio M. ; Jackson, R.L.</creator><creatorcontrib>Bradley, W.A. ; Rohde, M.F. ; Gotto, Antonio M. ; Jackson, R.L.</creatorcontrib><description>After >95% cleavage of the apoprotein (apoB) of the low density lipoproteins with cyanogen bromide, the peptides produced are shown to be extensively aggregated in sodium dodecyl sulfate. Both high temperature and increased concentration (5%) of the detergent are necessary to shift the aggregated peptides from high molecular weight (>25,000) to lower molecular weight aggregates as seen on sodium dodecyl sulfate polyacrylamide gel electrophoresis. End group analyses of the cyanogen bromide digestion by automated sequencer techniques indicate the presence of five (5) methionines. With a known methionine content of 16 moles/100,000 g protein, the molecular weight of the apoprotein must be approximately 30,000.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(78)91440-7</identifier><identifier>PMID: 208540</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Amino Acids - analysis ; Apolipoproteins - blood ; Cyanogen Bromide ; Humans ; Lipoproteins, LDL - blood ; Molecular Weight ; Peptide Fragments - analysis</subject><ispartof>Biochemical and biophysical research communications, 1978-04, Vol.81 (3), p.928-935</ispartof><rights>1978</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-e939fe40d11ef249eed3719dd3448ca20f4aef4f65b088995f38ca9ad8091a363</citedby><cites>FETCH-LOGICAL-c356t-e939fe40d11ef249eed3719dd3448ca20f4aef4f65b088995f38ca9ad8091a363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(78)91440-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/208540$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bradley, W.A.</creatorcontrib><creatorcontrib>Rohde, M.F.</creatorcontrib><creatorcontrib>Gotto, Antonio M.</creatorcontrib><creatorcontrib>Jackson, R.L.</creatorcontrib><title>The cyanogen bromide peptides of the apoprotein of low density lipoprotein (apoB): Its molecular weight from a chemical view</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>After >95% cleavage of the apoprotein (apoB) of the low density lipoproteins with cyanogen bromide, the peptides produced are shown to be extensively aggregated in sodium dodecyl sulfate. Both high temperature and increased concentration (5%) of the detergent are necessary to shift the aggregated peptides from high molecular weight (>25,000) to lower molecular weight aggregates as seen on sodium dodecyl sulfate polyacrylamide gel electrophoresis. End group analyses of the cyanogen bromide digestion by automated sequencer techniques indicate the presence of five (5) methionines. With a known methionine content of 16 moles/100,000 g protein, the molecular weight of the apoprotein must be approximately 30,000.</description><subject>Amino Acid Sequence</subject><subject>Amino Acids - analysis</subject><subject>Apolipoproteins - blood</subject><subject>Cyanogen Bromide</subject><subject>Humans</subject><subject>Lipoproteins, LDL - blood</subject><subject>Molecular Weight</subject><subject>Peptide Fragments - analysis</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9P3DAQxa2qtN3SfgMOPiE4pIwT5485IBUELRISF5B6s7z2mDVK4mB7u1qJD4_DInrraaR5v3ma9wg5YPCDAWtOAKApSsH-HLXdsWCcQ9F-IAsGAoqSAf9IFu_IF_I1xkcAxngjPpNPJXQ1hwV5vlsh1Vs1-gcc6TL4wRmkE04pz0i9pSkDavJT8AndOG96v6EGx-jSlvbun3SUsfPjU3qdIh18j3rdq0A36B5WidpsTRXVKxycVj3963DzjexZ1Uf8_jb3yf3V5d3F7-Lm9tf1xc-bQld1kwoUlbDIwTCGtuQC0VQtE8ZUnHdalWC5QsttUy-h64SobZXXQpkOBFNVU-2Tw51v_vRpjTHJwUWNfa9G9OsoWw685qzNIN-BOvgYA1o5BTeosJUM5Ny5nAuVc6Gy7eRr53I-O3jzXy8HNO9Hu5KzfLaTMWfMuYOM2uGo0biAOknj3f_9XwA_bJI7</recordid><startdate>19780414</startdate><enddate>19780414</enddate><creator>Bradley, W.A.</creator><creator>Rohde, M.F.</creator><creator>Gotto, Antonio M.</creator><creator>Jackson, R.L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19780414</creationdate><title>The cyanogen bromide peptides of the apoprotein of low density lipoprotein (apoB): Its molecular weight from a chemical view</title><author>Bradley, W.A. ; Rohde, M.F. ; Gotto, Antonio M. ; Jackson, R.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-e939fe40d11ef249eed3719dd3448ca20f4aef4f65b088995f38ca9ad8091a363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Amino Acid Sequence</topic><topic>Amino Acids - analysis</topic><topic>Apolipoproteins - blood</topic><topic>Cyanogen Bromide</topic><topic>Humans</topic><topic>Lipoproteins, LDL - blood</topic><topic>Molecular Weight</topic><topic>Peptide Fragments - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bradley, W.A.</creatorcontrib><creatorcontrib>Rohde, M.F.</creatorcontrib><creatorcontrib>Gotto, Antonio M.</creatorcontrib><creatorcontrib>Jackson, R.L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bradley, W.A.</au><au>Rohde, M.F.</au><au>Gotto, Antonio M.</au><au>Jackson, R.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The cyanogen bromide peptides of the apoprotein of low density lipoprotein (apoB): Its molecular weight from a chemical view</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1978-04-14</date><risdate>1978</risdate><volume>81</volume><issue>3</issue><spage>928</spage><epage>935</epage><pages>928-935</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>After >95% cleavage of the apoprotein (apoB) of the low density lipoproteins with cyanogen bromide, the peptides produced are shown to be extensively aggregated in sodium dodecyl sulfate. Both high temperature and increased concentration (5%) of the detergent are necessary to shift the aggregated peptides from high molecular weight (>25,000) to lower molecular weight aggregates as seen on sodium dodecyl sulfate polyacrylamide gel electrophoresis. End group analyses of the cyanogen bromide digestion by automated sequencer techniques indicate the presence of five (5) methionines. With a known methionine content of 16 moles/100,000 g protein, the molecular weight of the apoprotein must be approximately 30,000.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>208540</pmid><doi>10.1016/0006-291X(78)91440-7</doi><tpages>8</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0006-291X
ispartof	Biochemical and biophysical research communications, 1978-04, Vol.81 (3), p.928-935
issn	0006-291X 1090-2104
language	eng
recordid	cdi_proquest_miscellaneous_74045417
source	MEDLINE; Elsevier ScienceDirect Journals Complete
subjects	Amino Acid Sequence Amino Acids - analysis Apolipoproteins - blood Cyanogen Bromide Humans Lipoproteins, LDL - blood Molecular Weight Peptide Fragments - analysis
title	The cyanogen bromide peptides of the apoprotein of low density lipoprotein (apoB): Its molecular weight from a chemical view
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T20%3A02%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20cyanogen%20bromide%20peptides%20of%20the%20apoprotein%20of%20low%20density%20lipoprotein%20(apoB):%20Its%20molecular%20weight%20from%20a%20chemical%20view&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Bradley,%20W.A.&rft.date=1978-04-14&rft.volume=81&rft.issue=3&rft.spage=928&rft.epage=935&rft.pages=928-935&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/0006-291X(78)91440-7&rft_dat=%3Cproquest_cross%3E74045417%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=74045417&rft_id=info:pmid/208540&rft_els_id=0006291X78914407&rfr_iscdi=true