Modified oscillation behavior and decreased d-3-hydroxybutyrate dehydrogenase activity in diabetic rat liver mitochondria
One month after induction of diabetes in adult white rats with streptozotocin or 4–10 months after its induction by pancreatectomy (in every case glycemia was over 3 g/liter), the following alterations were observed in liver mitochondria: (a) a decrease of amplitude and an increase of the damping fa...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1982-04, Vol.214 (2), p.581-588 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | Brignone, J.A. de Brignone, Clara M.Campos Rodriguez, R.R. Badano, Blanca N. Stoppani, A.O.M. |
| description | One month after induction of diabetes in adult white rats with streptozotocin or 4–10 months after its induction by pancreatectomy (in every case glycemia was over 3 g/liter), the following alterations were observed in liver mitochondria: (a) a decrease of amplitude and an increase of the damping factor of volume oscillations induced by potassium ions and valinomycin; (b) a 50% decrease of
d-3-hydroxybutyrate dehydrogenase (HBD) activity in mitochondria disrupted by repeated freeze-thawing; (c) a similar decrease in the rate of
d-3-hydroxybutyrate oxidation by intact mitochondria; (d) a significant increase of cytochrome oxidase activity and cytochrome
aa
3 content. Measurement of succinate dehydrogenase and NADH dehydrogenase activity, the cytochrome
b,
c
1, and
c content, and the P:O ratio for mitochondria oxidizing
d-3-hydroxybutyrate did not reveal significant differences between control and diabetic rat mitochondria. In the streptozotocin-injected rats, the variation of HBD activity and the modification of the mitochondrial oscillation pattern were time-dependent phenomena, both effects reaching their maximal expression about 1 month after the onset of diabetes. The variation of HBD activity followed a biphasic course, since it rose to above the control level during the first 2 weeks of diabetes, then fell progressively to about half the control value after the third week. Treatment of diabetic rats with NPH insulin (5 IU twice daily, for 3 days, reinforced by the same dose 45 min before sacrifice) restored the mitochondrial oscillation pattern, HBD activity, and rate of
d-3-hydroxybutyrate oxidation by intact mitochondria to their normal values. |
| doi_str_mv | 10.1016/0003-9861(82)90063-7 |
| format | Article |
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d-3-hydroxybutyrate dehydrogenase (HBD) activity in mitochondria disrupted by repeated freeze-thawing; (c) a similar decrease in the rate of
d-3-hydroxybutyrate oxidation by intact mitochondria; (d) a significant increase of cytochrome oxidase activity and cytochrome
aa
3 content. Measurement of succinate dehydrogenase and NADH dehydrogenase activity, the cytochrome
b,
c
1, and
c content, and the P:O ratio for mitochondria oxidizing
d-3-hydroxybutyrate did not reveal significant differences between control and diabetic rat mitochondria. In the streptozotocin-injected rats, the variation of HBD activity and the modification of the mitochondrial oscillation pattern were time-dependent phenomena, both effects reaching their maximal expression about 1 month after the onset of diabetes. The variation of HBD activity followed a biphasic course, since it rose to above the control level during the first 2 weeks of diabetes, then fell progressively to about half the control value after the third week. Treatment of diabetic rats with NPH insulin (5 IU twice daily, for 3 days, reinforced by the same dose 45 min before sacrifice) restored the mitochondrial oscillation pattern, HBD activity, and rate of
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d-3-hydroxybutyrate dehydrogenase (HBD) activity in mitochondria disrupted by repeated freeze-thawing; (c) a similar decrease in the rate of
d-3-hydroxybutyrate oxidation by intact mitochondria; (d) a significant increase of cytochrome oxidase activity and cytochrome
aa
3 content. Measurement of succinate dehydrogenase and NADH dehydrogenase activity, the cytochrome
b,
c
1, and
c content, and the P:O ratio for mitochondria oxidizing
d-3-hydroxybutyrate did not reveal significant differences between control and diabetic rat mitochondria. In the streptozotocin-injected rats, the variation of HBD activity and the modification of the mitochondrial oscillation pattern were time-dependent phenomena, both effects reaching their maximal expression about 1 month after the onset of diabetes. The variation of HBD activity followed a biphasic course, since it rose to above the control level during the first 2 weeks of diabetes, then fell progressively to about half the control value after the third week. Treatment of diabetic rats with NPH insulin (5 IU twice daily, for 3 days, reinforced by the same dose 45 min before sacrifice) restored the mitochondrial oscillation pattern, HBD activity, and rate of
d-3-hydroxybutyrate oxidation by intact mitochondria to their normal values.</description><subject>Animals</subject><subject>Diabetes Mellitus, Experimental - drug therapy</subject><subject>Diabetes Mellitus, Experimental - enzymology</subject><subject>Electron Transport Complex IV - metabolism</subject><subject>Hydroxybutyrate Dehydrogenase - metabolism</subject><subject>Insulin - therapeutic use</subject><subject>Kinetics</subject><subject>Male</subject><subject>Mitochondria, Liver - enzymology</subject><subject>Mitochondrial Swelling</subject><subject>Oxygen Consumption</subject><subject>Pancreatectomy</subject><subject>Rats</subject><subject>Spectrophotometry</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVoSDcf_6AFnUpzcDqSbNm-FEJI2kJCLslZyNI4O2XXSiXtUv_7arNLjj0NzPvMDPMw9knAlQChvwGAqvpOi6-dvOwBtKraI7YQ0OsKVFd_YIt35CM7Tek3gBC1lifsRMuuBtkt2PwQPI2EnofkaLWymcLEB1zaLYXI7eS5RxfRpoL4SlXL2cfwdx42eY42Y0nfOi84FYRbl2lLeeY0cU92wEyOF46vaIuRrykHtwyTj2TP2fFoVwkvDvWMPd_dPt38rO4ff_y6ub6vnGraXMkenAYxStk7i72Tgxwb6K2VQjZOSS9lK9XQNlqPWvcgWhy6UVnhxsHqRqkz9mW_9zWGPxtM2awpOSyvThg2ybQ11FLotoD1HnQxpBRxNK-R1jbORoDZGTc7nWan03TSvBk3u7HPh_2bYY3-feiguOTf9zmWJ7eE0RTRODn0FNFl4wP9_8A_DEWR2A</recordid><startdate>19820401</startdate><enddate>19820401</enddate><creator>Brignone, J.A.</creator><creator>de Brignone, Clara M.Campos</creator><creator>Rodriguez, R.R.</creator><creator>Badano, Blanca N.</creator><creator>Stoppani, A.O.M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19820401</creationdate><title>Modified oscillation behavior and decreased d-3-hydroxybutyrate dehydrogenase activity in diabetic rat liver mitochondria</title><author>Brignone, J.A. ; de Brignone, Clara M.Campos ; Rodriguez, R.R. ; Badano, Blanca N. ; Stoppani, A.O.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-290c601f229cae9c2b2f509aa2125c32d22723b7566f669017eb8f3a1cfba6533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Diabetes Mellitus, Experimental - drug therapy</topic><topic>Diabetes Mellitus, Experimental - enzymology</topic><topic>Electron Transport Complex IV - metabolism</topic><topic>Hydroxybutyrate Dehydrogenase - metabolism</topic><topic>Insulin - therapeutic use</topic><topic>Kinetics</topic><topic>Male</topic><topic>Mitochondria, Liver - enzymology</topic><topic>Mitochondrial Swelling</topic><topic>Oxygen Consumption</topic><topic>Pancreatectomy</topic><topic>Rats</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brignone, J.A.</creatorcontrib><creatorcontrib>de Brignone, Clara M.Campos</creatorcontrib><creatorcontrib>Rodriguez, R.R.</creatorcontrib><creatorcontrib>Badano, Blanca N.</creatorcontrib><creatorcontrib>Stoppani, A.O.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brignone, J.A.</au><au>de Brignone, Clara M.Campos</au><au>Rodriguez, R.R.</au><au>Badano, Blanca N.</au><au>Stoppani, A.O.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modified oscillation behavior and decreased d-3-hydroxybutyrate dehydrogenase activity in diabetic rat liver mitochondria</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1982-04-01</date><risdate>1982</risdate><volume>214</volume><issue>2</issue><spage>581</spage><epage>588</epage><pages>581-588</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>One month after induction of diabetes in adult white rats with streptozotocin or 4–10 months after its induction by pancreatectomy (in every case glycemia was over 3 g/liter), the following alterations were observed in liver mitochondria: (a) a decrease of amplitude and an increase of the damping factor of volume oscillations induced by potassium ions and valinomycin; (b) a 50% decrease of
d-3-hydroxybutyrate dehydrogenase (HBD) activity in mitochondria disrupted by repeated freeze-thawing; (c) a similar decrease in the rate of
d-3-hydroxybutyrate oxidation by intact mitochondria; (d) a significant increase of cytochrome oxidase activity and cytochrome
aa
3 content. Measurement of succinate dehydrogenase and NADH dehydrogenase activity, the cytochrome
b,
c
1, and
c content, and the P:O ratio for mitochondria oxidizing
d-3-hydroxybutyrate did not reveal significant differences between control and diabetic rat mitochondria. In the streptozotocin-injected rats, the variation of HBD activity and the modification of the mitochondrial oscillation pattern were time-dependent phenomena, both effects reaching their maximal expression about 1 month after the onset of diabetes. The variation of HBD activity followed a biphasic course, since it rose to above the control level during the first 2 weeks of diabetes, then fell progressively to about half the control value after the third week. Treatment of diabetic rats with NPH insulin (5 IU twice daily, for 3 days, reinforced by the same dose 45 min before sacrifice) restored the mitochondrial oscillation pattern, HBD activity, and rate of
d-3-hydroxybutyrate oxidation by intact mitochondria to their normal values.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6284028</pmid><doi>10.1016/0003-9861(82)90063-7</doi><tpages>8</tpages></addata></record> |
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| ispartof | Archives of biochemistry and biophysics, 1982-04, Vol.214 (2), p.581-588 |
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| subjects | Animals Diabetes Mellitus, Experimental - drug therapy Diabetes Mellitus, Experimental - enzymology Electron Transport Complex IV - metabolism Hydroxybutyrate Dehydrogenase - metabolism Insulin - therapeutic use Kinetics Male Mitochondria, Liver - enzymology Mitochondrial Swelling Oxygen Consumption Pancreatectomy Rats Spectrophotometry |
| title | Modified oscillation behavior and decreased d-3-hydroxybutyrate dehydrogenase activity in diabetic rat liver mitochondria |
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