A novel reversible thiol-specific spin label: Papain active site labeling and inhibition

A new, highly reactive, thiol-specific spin label, (1-oxyl-2,2,5,5-tetramethyl-Δ 3-pyrroline-3-methyl)methanethiosulfonate was synthesized. Its unique specificity was demonstrated with the active thiol protease, papain, which was stoichiometrically inhibited within 5 min, resulting in a conformation...

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Veröffentlicht in:	Analytical biochemistry 1982-01, Vol.119 (2), p.450-455
Hauptverfasser:	Berliner, Lawrence J., Grunwald, Jacob, Hankovszky, H.Olga, Hideg, Kalman
Format:	Artikel
Sprache:	eng
Schlagworte:	active sites Binding Sites Chemical Phenomena Chemistry Cyclic N-Oxides - chemical synthesis Electron Spin Resonance Spectroscopy - methods Enzyme Activation Hydrogen-Ion Concentration Mesylates papain Papain - analysis Papain - antagonists & inhibitors spin labelling Spin Labels - chemical synthesis Sulfhydryl Compounds - analysis
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