N-acetyl- β-hexosaminidase activity in human breast milk
1. 1. The lysosomal enzyme, N-acetyl- β-hexosaminidase (HEX) is present in human breast milk. It is composed predominantly of “A” (heat-labile) and “B” (heat-stable) isozymes which coelute with the corresponding major serum isozymes on DE-52 ion-exchange chromatography. 2. 2. Total HEX activity in “...
Gespeichert in:
| Veröffentlicht in: | International journal of biochemistry 1982, Vol.14 (2), p.151-154 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 154 |
|---|---|
| container_issue | 2 |
| container_start_page | 151 |
| container_title | International journal of biochemistry |
| container_volume | 14 |
| creator | Oberkotter, Linda V. Koldovsky, Otakar Tenore, Alfred |
| description | 1.
1. The lysosomal enzyme,
N-acetyl-
β-hexosaminidase (HEX) is present in human breast milk. It is composed predominantly of “A” (heat-labile) and “B” (heat-stable) isozymes which coelute with the corresponding major serum isozymes on DE-52 ion-exchange chromatography.
2.
2. Total HEX activity in “early” milk obtained at 2.8 ± 1.4 weeks
post partum, is approx. 2.5-fold higher (87 ± 29 nmol/60'/mg protein,
n = 10) than that of pregnancy serum (35.7 nmol/60'/mg protein) prior to delivery.
3.
3. These levels increase to greater than 3-fold (110 ± 20 nmol/60'/ mg protein,
n = 13) as the milk matures (10.3 ± 4.2 weeks).
4.
4. The specific activity of HEX A in the milk changes little with time
post partum, because absolute levels are decreasing simultaneous with decreases in milk protein.
5.
5. In contrast, HEX B specific activity is increased, as absolute levels (per volume) remain constant in the face of decreasing milk protein content.
6.
6. These changes result in a high degree of correlation (
r = 0.81) between time of lactation and % HEX A observed. |
| doi_str_mv | 10.1016/0020-711X(82)90155-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74017125</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0020711X82901550</els_id><sourcerecordid>74017125</sourcerecordid><originalsourceid>FETCH-LOGICAL-c357t-a2161f8a61c5d242fed8fe730f69703055a07d1593543f0914ce17d9c7307a963</originalsourceid><addsrcrecordid>eNp9kEtOwzAQhr0AlVK4AUhZIVgEZpLYjjdIqOIlVbABiZ3lOhPVkEex04pei4NwJlJadclqpPkfo_kYO0G4REBxBZBALBHfzvPkQgFyHsMeG-7WB-wwhHcAVHmGAzYQmUAu5JCpp9hY6lZVHP18xzP6aoOpXeMKEygytnNL160i10SzRW2aaOrJhC6qXfVxxPZLUwU63s4Re727fRk_xJPn-8fxzSS2KZddbBIUWOZGoOVFkiUlFXlJMoVSKAkpcG5AFshVyrO0BIWZJZSFsr1FGiXSETvb9M59-7mg0OnaBUtVZRpqF0HLDFBiwntjtjFa34bgqdRz72rjVxpBrynpNQ69xqHzRP9R0tDHTrf9i2lNxS60RdTr1xud-ieXjrwO1lFjqXCebKeL1v1_4BegK3d8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74017125</pqid></control><display><type>article</type><title>N-acetyl- β-hexosaminidase activity in human breast milk</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Oberkotter, Linda V. ; Koldovsky, Otakar ; Tenore, Alfred</creator><creatorcontrib>Oberkotter, Linda V. ; Koldovsky, Otakar ; Tenore, Alfred</creatorcontrib><description>1.
1. The lysosomal enzyme,
N-acetyl-
β-hexosaminidase (HEX) is present in human breast milk. It is composed predominantly of “A” (heat-labile) and “B” (heat-stable) isozymes which coelute with the corresponding major serum isozymes on DE-52 ion-exchange chromatography.
2.
2. Total HEX activity in “early” milk obtained at 2.8 ± 1.4 weeks
post partum, is approx. 2.5-fold higher (87 ± 29 nmol/60'/mg protein,
n = 10) than that of pregnancy serum (35.7 nmol/60'/mg protein) prior to delivery.
3.
3. These levels increase to greater than 3-fold (110 ± 20 nmol/60'/ mg protein,
n = 13) as the milk matures (10.3 ± 4.2 weeks).
4.
4. The specific activity of HEX A in the milk changes little with time
post partum, because absolute levels are decreasing simultaneous with decreases in milk protein.
5.
5. In contrast, HEX B specific activity is increased, as absolute levels (per volume) remain constant in the face of decreasing milk protein content.
6.
6. These changes result in a high degree of correlation (
r = 0.81) between time of lactation and % HEX A observed.</description><identifier>ISSN: 0020-711X</identifier><identifier>DOI: 10.1016/0020-711X(82)90155-0</identifier><identifier>PMID: 6461567</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Adolescent ; Adult ; beta-N-Acetylhexosaminidases ; Female ; Hexosaminidase A ; Hexosaminidase B ; Hexosaminidases - blood ; Hexosaminidases - metabolism ; Humans ; Isoenzymes - metabolism ; Lactation ; Milk Proteins - metabolism ; Milk, Human - enzymology ; Postpartum Period ; Pregnancy ; Time Factors</subject><ispartof>International journal of biochemistry, 1982, Vol.14 (2), p.151-154</ispartof><rights>1982</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-a2161f8a61c5d242fed8fe730f69703055a07d1593543f0914ce17d9c7307a963</citedby><cites>FETCH-LOGICAL-c357t-a2161f8a61c5d242fed8fe730f69703055a07d1593543f0914ce17d9c7307a963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6461567$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oberkotter, Linda V.</creatorcontrib><creatorcontrib>Koldovsky, Otakar</creatorcontrib><creatorcontrib>Tenore, Alfred</creatorcontrib><title>N-acetyl- β-hexosaminidase activity in human breast milk</title><title>International journal of biochemistry</title><addtitle>Int J Biochem</addtitle><description>1.
1. The lysosomal enzyme,
N-acetyl-
β-hexosaminidase (HEX) is present in human breast milk. It is composed predominantly of “A” (heat-labile) and “B” (heat-stable) isozymes which coelute with the corresponding major serum isozymes on DE-52 ion-exchange chromatography.
2.
2. Total HEX activity in “early” milk obtained at 2.8 ± 1.4 weeks
post partum, is approx. 2.5-fold higher (87 ± 29 nmol/60'/mg protein,
n = 10) than that of pregnancy serum (35.7 nmol/60'/mg protein) prior to delivery.
3.
3. These levels increase to greater than 3-fold (110 ± 20 nmol/60'/ mg protein,
n = 13) as the milk matures (10.3 ± 4.2 weeks).
4.
4. The specific activity of HEX A in the milk changes little with time
post partum, because absolute levels are decreasing simultaneous with decreases in milk protein.
5.
5. In contrast, HEX B specific activity is increased, as absolute levels (per volume) remain constant in the face of decreasing milk protein content.
6.
6. These changes result in a high degree of correlation (
r = 0.81) between time of lactation and % HEX A observed.</description><subject>Adolescent</subject><subject>Adult</subject><subject>beta-N-Acetylhexosaminidases</subject><subject>Female</subject><subject>Hexosaminidase A</subject><subject>Hexosaminidase B</subject><subject>Hexosaminidases - blood</subject><subject>Hexosaminidases - metabolism</subject><subject>Humans</subject><subject>Isoenzymes - metabolism</subject><subject>Lactation</subject><subject>Milk Proteins - metabolism</subject><subject>Milk, Human - enzymology</subject><subject>Postpartum Period</subject><subject>Pregnancy</subject><subject>Time Factors</subject><issn>0020-711X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtOwzAQhr0AlVK4AUhZIVgEZpLYjjdIqOIlVbABiZ3lOhPVkEex04pei4NwJlJadclqpPkfo_kYO0G4REBxBZBALBHfzvPkQgFyHsMeG-7WB-wwhHcAVHmGAzYQmUAu5JCpp9hY6lZVHP18xzP6aoOpXeMKEygytnNL160i10SzRW2aaOrJhC6qXfVxxPZLUwU63s4Re727fRk_xJPn-8fxzSS2KZddbBIUWOZGoOVFkiUlFXlJMoVSKAkpcG5AFshVyrO0BIWZJZSFsr1FGiXSETvb9M59-7mg0OnaBUtVZRpqF0HLDFBiwntjtjFa34bgqdRz72rjVxpBrynpNQ69xqHzRP9R0tDHTrf9i2lNxS60RdTr1xud-ieXjrwO1lFjqXCebKeL1v1_4BegK3d8</recordid><startdate>1982</startdate><enddate>1982</enddate><creator>Oberkotter, Linda V.</creator><creator>Koldovsky, Otakar</creator><creator>Tenore, Alfred</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1982</creationdate><title>N-acetyl- β-hexosaminidase activity in human breast milk</title><author>Oberkotter, Linda V. ; Koldovsky, Otakar ; Tenore, Alfred</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-a2161f8a61c5d242fed8fe730f69703055a07d1593543f0914ce17d9c7307a963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>beta-N-Acetylhexosaminidases</topic><topic>Female</topic><topic>Hexosaminidase A</topic><topic>Hexosaminidase B</topic><topic>Hexosaminidases - blood</topic><topic>Hexosaminidases - metabolism</topic><topic>Humans</topic><topic>Isoenzymes - metabolism</topic><topic>Lactation</topic><topic>Milk Proteins - metabolism</topic><topic>Milk, Human - enzymology</topic><topic>Postpartum Period</topic><topic>Pregnancy</topic><topic>Time Factors</topic><toplevel>online_resources</toplevel><creatorcontrib>Oberkotter, Linda V.</creatorcontrib><creatorcontrib>Koldovsky, Otakar</creatorcontrib><creatorcontrib>Tenore, Alfred</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oberkotter, Linda V.</au><au>Koldovsky, Otakar</au><au>Tenore, Alfred</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>N-acetyl- β-hexosaminidase activity in human breast milk</atitle><jtitle>International journal of biochemistry</jtitle><addtitle>Int J Biochem</addtitle><date>1982</date><risdate>1982</risdate><volume>14</volume><issue>2</issue><spage>151</spage><epage>154</epage><pages>151-154</pages><issn>0020-711X</issn><abstract>1.
1. The lysosomal enzyme,
N-acetyl-
β-hexosaminidase (HEX) is present in human breast milk. It is composed predominantly of “A” (heat-labile) and “B” (heat-stable) isozymes which coelute with the corresponding major serum isozymes on DE-52 ion-exchange chromatography.
2.
2. Total HEX activity in “early” milk obtained at 2.8 ± 1.4 weeks
post partum, is approx. 2.5-fold higher (87 ± 29 nmol/60'/mg protein,
n = 10) than that of pregnancy serum (35.7 nmol/60'/mg protein) prior to delivery.
3.
3. These levels increase to greater than 3-fold (110 ± 20 nmol/60'/ mg protein,
n = 13) as the milk matures (10.3 ± 4.2 weeks).
4.
4. The specific activity of HEX A in the milk changes little with time
post partum, because absolute levels are decreasing simultaneous with decreases in milk protein.
5.
5. In contrast, HEX B specific activity is increased, as absolute levels (per volume) remain constant in the face of decreasing milk protein content.
6.
6. These changes result in a high degree of correlation (
r = 0.81) between time of lactation and % HEX A observed.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>6461567</pmid><doi>10.1016/0020-711X(82)90155-0</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0020-711X |
| ispartof | International journal of biochemistry, 1982, Vol.14 (2), p.151-154 |
| issn | 0020-711X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74017125 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adolescent Adult beta-N-Acetylhexosaminidases Female Hexosaminidase A Hexosaminidase B Hexosaminidases - blood Hexosaminidases - metabolism Humans Isoenzymes - metabolism Lactation Milk Proteins - metabolism Milk, Human - enzymology Postpartum Period Pregnancy Time Factors |
| title | N-acetyl- β-hexosaminidase activity in human breast milk |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T08%3A37%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=N-acetyl-%20%CE%B2-hexosaminidase%20activity%20in%20human%20breast%20milk&rft.jtitle=International%20journal%20of%20biochemistry&rft.au=Oberkotter,%20Linda%20V.&rft.date=1982&rft.volume=14&rft.issue=2&rft.spage=151&rft.epage=154&rft.pages=151-154&rft.issn=0020-711X&rft_id=info:doi/10.1016/0020-711X(82)90155-0&rft_dat=%3Cproquest_cross%3E74017125%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=74017125&rft_id=info:pmid/6461567&rft_els_id=0020711X82901550&rfr_iscdi=true |