Thymopoietin Enhances the Allogeneic Response and Cyclic GMP Levels of Mouse Peripheral, Thymus-Derived Lymphocytes
The action of the purified thymic factor, thymopoietin, on populations of post-thymic lymphocytes has been studied. Thymopoietin, at concentrations as low as 1.5 ng/ml, uniquely enhanced the proliferative response of peripheral T cells from lymph node and spleen to allogeneic stimulation. Enhancemen...
Gespeichert in:
| Veröffentlicht in: | The Journal of immunology (1950) 1978-05, Vol.120 (5), p.1594-1599 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1599 |
|---|---|
| container_issue | 5 |
| container_start_page | 1594 |
| container_title | The Journal of immunology (1950) |
| container_volume | 120 |
| creator | Sunshine, Geoffrey H Basch, Ross S Coffey, Ronald G Cohen, Kenneth W Goldstein, Gideon Hadden, John W |
| description | The action of the purified thymic factor, thymopoietin, on populations of post-thymic lymphocytes has been studied. Thymopoietin, at concentrations as low as 1.5 ng/ml, uniquely enhanced the proliferative response of peripheral T cells from lymph node and spleen to allogeneic stimulation. Enhancement of the allogeneic response (MLR) was not produced by several polypeptide hormones, including insulin, ACTH, HCG, or Ubiquitin. Treatment of spleen cells with anti-Thy-1 antiserum almost completely abolished the MLR. Thymopoietin's stimulatory effects could not reverse this. Thymopoietin treatment of Thy-1+-enriched spleen cell populations enhanced the MLR even when thymopoietin was removed as early as 2 min after incubation with responding cells. The interaction of thymopoietin with peripheral Thy-1+ cell populations produced a rapid and transient rise in cyclic GMP levels and slightly decreased cyclic AMP levels. These results suggest that thymopoietin interacts with one or more Thy-1+ subpopulations and that this interaction involves early changes in cyclic nucleotide metabolism. |
| doi_str_mv | 10.4049/jimmunol.120.5.1594 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_74007921</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>74007921</sourcerecordid><originalsourceid>FETCH-LOGICAL-c376t-ca31dbcd9d65bfd8dac7be732f051c206710b81b6a49de31feb8f31507dfb4de3</originalsourceid><addsrcrecordid>eNpNkMtO3DAUhi1U2g6UJ2gXXsGGDMe52JMlmnKTBoEQrC3HPiFGTpzGCaO8fT0aWrE60n_T0UfITwbLHPLy4s227dR5t2QpLIslK8r8gCxYUUDCOfAvZAGQpgkTXHwnRyG8AQCHNP9GvqYghMgWJDw3c-t7b3G0Hb3qGtVpDHRskF4651-xQ6vpE4bedwGp6gxdz9pF7eb-kW7wHV2gvqb3for2Iw62b3BQ7pzuhqeQ_I7SOxq6mdu-8XoeMfwgh7VyAU8-7jF5ub56Xt8mm4ebu_XlJtGZ4GOiVcZMpU1peFHVZmWUFhWKLK2hYDoFLhhUK1ZxlZcGM1ZjtaozVoAwdZVH5Zic7nf7wf-ZMIyytUGjc6rD-K4UOYAoUxaD2T6oBx_CgLXsB9uqYZYM5I60_EdaRtKykDvSsfXrY36qWjT_O3u00T7b2419bbZ2QBla5VwMM7ndbj8N_QXoHowO</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>74007921</pqid></control><display><type>article</type><title>Thymopoietin Enhances the Allogeneic Response and Cyclic GMP Levels of Mouse Peripheral, Thymus-Derived Lymphocytes</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Sunshine, Geoffrey H ; Basch, Ross S ; Coffey, Ronald G ; Cohen, Kenneth W ; Goldstein, Gideon ; Hadden, John W</creator><creatorcontrib>Sunshine, Geoffrey H ; Basch, Ross S ; Coffey, Ronald G ; Cohen, Kenneth W ; Goldstein, Gideon ; Hadden, John W</creatorcontrib><description>The action of the purified thymic factor, thymopoietin, on populations of post-thymic lymphocytes has been studied. Thymopoietin, at concentrations as low as 1.5 ng/ml, uniquely enhanced the proliferative response of peripheral T cells from lymph node and spleen to allogeneic stimulation. Enhancement of the allogeneic response (MLR) was not produced by several polypeptide hormones, including insulin, ACTH, HCG, or Ubiquitin. Treatment of spleen cells with anti-Thy-1 antiserum almost completely abolished the MLR. Thymopoietin's stimulatory effects could not reverse this. Thymopoietin treatment of Thy-1+-enriched spleen cell populations enhanced the MLR even when thymopoietin was removed as early as 2 min after incubation with responding cells. The interaction of thymopoietin with peripheral Thy-1+ cell populations produced a rapid and transient rise in cyclic GMP levels and slightly decreased cyclic AMP levels. These results suggest that thymopoietin interacts with one or more Thy-1+ subpopulations and that this interaction involves early changes in cyclic nucleotide metabolism.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.120.5.1594</identifier><identifier>PMID: 207773</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Animals ; Cyclic GMP - biosynthesis ; Dose-Response Relationship, Immunologic ; Lymph Nodes - immunology ; Lymphocyte Activation ; Lymphocyte Culture Test, Mixed ; Mice ; Mice, Inbred AKR ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Peptides - pharmacology ; Spleen - immunology ; T-Lymphocytes - immunology ; Thymopoietins - pharmacology ; Thymus Hormones - pharmacology</subject><ispartof>The Journal of immunology (1950), 1978-05, Vol.120 (5), p.1594-1599</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-ca31dbcd9d65bfd8dac7be732f051c206710b81b6a49de31feb8f31507dfb4de3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/207773$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sunshine, Geoffrey H</creatorcontrib><creatorcontrib>Basch, Ross S</creatorcontrib><creatorcontrib>Coffey, Ronald G</creatorcontrib><creatorcontrib>Cohen, Kenneth W</creatorcontrib><creatorcontrib>Goldstein, Gideon</creatorcontrib><creatorcontrib>Hadden, John W</creatorcontrib><title>Thymopoietin Enhances the Allogeneic Response and Cyclic GMP Levels of Mouse Peripheral, Thymus-Derived Lymphocytes</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>The action of the purified thymic factor, thymopoietin, on populations of post-thymic lymphocytes has been studied. Thymopoietin, at concentrations as low as 1.5 ng/ml, uniquely enhanced the proliferative response of peripheral T cells from lymph node and spleen to allogeneic stimulation. Enhancement of the allogeneic response (MLR) was not produced by several polypeptide hormones, including insulin, ACTH, HCG, or Ubiquitin. Treatment of spleen cells with anti-Thy-1 antiserum almost completely abolished the MLR. Thymopoietin's stimulatory effects could not reverse this. Thymopoietin treatment of Thy-1+-enriched spleen cell populations enhanced the MLR even when thymopoietin was removed as early as 2 min after incubation with responding cells. The interaction of thymopoietin with peripheral Thy-1+ cell populations produced a rapid and transient rise in cyclic GMP levels and slightly decreased cyclic AMP levels. These results suggest that thymopoietin interacts with one or more Thy-1+ subpopulations and that this interaction involves early changes in cyclic nucleotide metabolism.</description><subject>Animals</subject><subject>Cyclic GMP - biosynthesis</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Lymph Nodes - immunology</subject><subject>Lymphocyte Activation</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Mice</subject><subject>Mice, Inbred AKR</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Peptides - pharmacology</subject><subject>Spleen - immunology</subject><subject>T-Lymphocytes - immunology</subject><subject>Thymopoietins - pharmacology</subject><subject>Thymus Hormones - pharmacology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkMtO3DAUhi1U2g6UJ2gXXsGGDMe52JMlmnKTBoEQrC3HPiFGTpzGCaO8fT0aWrE60n_T0UfITwbLHPLy4s227dR5t2QpLIslK8r8gCxYUUDCOfAvZAGQpgkTXHwnRyG8AQCHNP9GvqYghMgWJDw3c-t7b3G0Hb3qGtVpDHRskF4651-xQ6vpE4bedwGp6gxdz9pF7eb-kW7wHV2gvqb3for2Iw62b3BQ7pzuhqeQ_I7SOxq6mdu-8XoeMfwgh7VyAU8-7jF5ub56Xt8mm4ebu_XlJtGZ4GOiVcZMpU1peFHVZmWUFhWKLK2hYDoFLhhUK1ZxlZcGM1ZjtaozVoAwdZVH5Zic7nf7wf-ZMIyytUGjc6rD-K4UOYAoUxaD2T6oBx_CgLXsB9uqYZYM5I60_EdaRtKykDvSsfXrY36qWjT_O3u00T7b2419bbZ2QBla5VwMM7ndbj8N_QXoHowO</recordid><startdate>197805</startdate><enddate>197805</enddate><creator>Sunshine, Geoffrey H</creator><creator>Basch, Ross S</creator><creator>Coffey, Ronald G</creator><creator>Cohen, Kenneth W</creator><creator>Goldstein, Gideon</creator><creator>Hadden, John W</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197805</creationdate><title>Thymopoietin Enhances the Allogeneic Response and Cyclic GMP Levels of Mouse Peripheral, Thymus-Derived Lymphocytes</title><author>Sunshine, Geoffrey H ; Basch, Ross S ; Coffey, Ronald G ; Cohen, Kenneth W ; Goldstein, Gideon ; Hadden, John W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-ca31dbcd9d65bfd8dac7be732f051c206710b81b6a49de31feb8f31507dfb4de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Animals</topic><topic>Cyclic GMP - biosynthesis</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>Lymph Nodes - immunology</topic><topic>Lymphocyte Activation</topic><topic>Lymphocyte Culture Test, Mixed</topic><topic>Mice</topic><topic>Mice, Inbred AKR</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Peptides - pharmacology</topic><topic>Spleen - immunology</topic><topic>T-Lymphocytes - immunology</topic><topic>Thymopoietins - pharmacology</topic><topic>Thymus Hormones - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sunshine, Geoffrey H</creatorcontrib><creatorcontrib>Basch, Ross S</creatorcontrib><creatorcontrib>Coffey, Ronald G</creatorcontrib><creatorcontrib>Cohen, Kenneth W</creatorcontrib><creatorcontrib>Goldstein, Gideon</creatorcontrib><creatorcontrib>Hadden, John W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sunshine, Geoffrey H</au><au>Basch, Ross S</au><au>Coffey, Ronald G</au><au>Cohen, Kenneth W</au><au>Goldstein, Gideon</au><au>Hadden, John W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thymopoietin Enhances the Allogeneic Response and Cyclic GMP Levels of Mouse Peripheral, Thymus-Derived Lymphocytes</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1978-05</date><risdate>1978</risdate><volume>120</volume><issue>5</issue><spage>1594</spage><epage>1599</epage><pages>1594-1599</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>The action of the purified thymic factor, thymopoietin, on populations of post-thymic lymphocytes has been studied. Thymopoietin, at concentrations as low as 1.5 ng/ml, uniquely enhanced the proliferative response of peripheral T cells from lymph node and spleen to allogeneic stimulation. Enhancement of the allogeneic response (MLR) was not produced by several polypeptide hormones, including insulin, ACTH, HCG, or Ubiquitin. Treatment of spleen cells with anti-Thy-1 antiserum almost completely abolished the MLR. Thymopoietin's stimulatory effects could not reverse this. Thymopoietin treatment of Thy-1+-enriched spleen cell populations enhanced the MLR even when thymopoietin was removed as early as 2 min after incubation with responding cells. The interaction of thymopoietin with peripheral Thy-1+ cell populations produced a rapid and transient rise in cyclic GMP levels and slightly decreased cyclic AMP levels. These results suggest that thymopoietin interacts with one or more Thy-1+ subpopulations and that this interaction involves early changes in cyclic nucleotide metabolism.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>207773</pmid><doi>10.4049/jimmunol.120.5.1594</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1978-05, Vol.120 (5), p.1594-1599 |
| issn | 0022-1767 1550-6606 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74007921 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cyclic GMP - biosynthesis Dose-Response Relationship, Immunologic Lymph Nodes - immunology Lymphocyte Activation Lymphocyte Culture Test, Mixed Mice Mice, Inbred AKR Mice, Inbred BALB C Mice, Inbred C57BL Peptides - pharmacology Spleen - immunology T-Lymphocytes - immunology Thymopoietins - pharmacology Thymus Hormones - pharmacology |
| title | Thymopoietin Enhances the Allogeneic Response and Cyclic GMP Levels of Mouse Peripheral, Thymus-Derived Lymphocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T17%3A26%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Thymopoietin%20Enhances%20the%20Allogeneic%20Response%20and%20Cyclic%20GMP%20Levels%20of%20Mouse%20Peripheral,%20Thymus-Derived%20Lymphocytes&rft.jtitle=The%20Journal%20of%20immunology%20(1950)&rft.au=Sunshine,%20Geoffrey%20H&rft.date=1978-05&rft.volume=120&rft.issue=5&rft.spage=1594&rft.epage=1599&rft.pages=1594-1599&rft.issn=0022-1767&rft.eissn=1550-6606&rft_id=info:doi/10.4049/jimmunol.120.5.1594&rft_dat=%3Cproquest_cross%3E74007921%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=74007921&rft_id=info:pmid/207773&rfr_iscdi=true |