Interaction of the carboxamide of NADPH with Lactobacillus casei dihydrofolate reductase
Dihydrofolate reductase from Lactobacillus casei and its complexes with NADPH and methotrexate yield well-resolved Raman spectra. The 1685-cm −1 Raman band assigned to the carboxamide of NADPH persists in the NADPH-enzyme binary complex but is absent from the NADPH-methotrexate-enzyme ternary comple...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1982, Vol.213 (1), p.338-340 |
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| container_end_page | 340 |
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| container_issue | 1 |
| container_start_page | 338 |
| container_title | Archives of biochemistry and biophysics |
| container_volume | 213 |
| creator | Dwivedi, Chandra M. Plante, Laurence T. Kisliuk, Roy L. Pastore, Edward J. Verma, Surendra P. Wallach, Donald F.H. |
| description | Dihydrofolate reductase from
Lactobacillus casei and its complexes with NADPH and methotrexate yield well-resolved Raman spectra. The 1685-cm
−1 Raman band assigned to the carboxamide of NADPH persists in the NADPH-enzyme binary complex but is absent from the NADPH-methotrexate-enzyme ternary complex. This is ascribed to stabilization of the polarized form of the carboxamide by H bonding to the NH and CO groups of Ala 6 and Ile 13 of the peptide backbone. |
| doi_str_mv | 10.1016/0003-9861(82)90471-4 |
| format | Article |
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Lactobacillus casei and its complexes with NADPH and methotrexate yield well-resolved Raman spectra. The 1685-cm
−1 Raman band assigned to the carboxamide of NADPH persists in the NADPH-enzyme binary complex but is absent from the NADPH-methotrexate-enzyme ternary complex. This is ascribed to stabilization of the polarized form of the carboxamide by H bonding to the NH and CO groups of Ala 6 and Ile 13 of the peptide backbone.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(82)90471-4</identifier><identifier>PMID: 6800305</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Lactobacillus casei - enzymology ; Methotrexate - metabolism ; NADP - metabolism ; Spectrum Analysis, Raman ; Tetrahydrofolate Dehydrogenase - metabolism</subject><ispartof>Archives of biochemistry and biophysics, 1982, Vol.213 (1), p.338-340</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c306t-da07ab8a02e5cc754b425ab696e0c61521f037ffa8839e666ec9ec548aba11e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(82)90471-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6800305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dwivedi, Chandra M.</creatorcontrib><creatorcontrib>Plante, Laurence T.</creatorcontrib><creatorcontrib>Kisliuk, Roy L.</creatorcontrib><creatorcontrib>Pastore, Edward J.</creatorcontrib><creatorcontrib>Verma, Surendra P.</creatorcontrib><creatorcontrib>Wallach, Donald F.H.</creatorcontrib><title>Interaction of the carboxamide of NADPH with Lactobacillus casei dihydrofolate reductase</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>Dihydrofolate reductase from
Lactobacillus casei and its complexes with NADPH and methotrexate yield well-resolved Raman spectra. The 1685-cm
−1 Raman band assigned to the carboxamide of NADPH persists in the NADPH-enzyme binary complex but is absent from the NADPH-methotrexate-enzyme ternary complex. This is ascribed to stabilization of the polarized form of the carboxamide by H bonding to the NH and CO groups of Ala 6 and Ile 13 of the peptide backbone.</description><subject>Lactobacillus casei - enzymology</subject><subject>Methotrexate - metabolism</subject><subject>NADP - metabolism</subject><subject>Spectrum Analysis, Raman</subject><subject>Tetrahydrofolate Dehydrogenase - metabolism</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo6_rxDxR6Ej1UJ22aphdhWT9hUQ8K3kKaTtlId6NJqu6_N3UXj54G5n1mhnkIOaJwToHyCwDI00pweiqyswpYSVO2RcYUKp5CLtg2Gf8hu2TP-zcAShnPRmTERUygGJPX-2VAp3QwdpnYNglzTLRytf1WC9Pg0HqYXD3dJV8mzJNZBG2ttOm63kfOo0kaM181zra2UwETh02vQwwOyE6rOo-Hm7pPXm6un6d36ezx9n46maU6Bx7SRkGpaqEgw0LrsmA1ywpV84ojaE6LjLaQl22rhMgr5JyjrlAXTKhaUYpFvk9O1nvfnf3o0Qe5MF5j16kl2t7LksVPOVQRZGtQO-u9w1a-O7NQbiUpyEGoHGzJwZYUmfwVKlkcO97s7-sFNn9DG4Mxv1znGJ_8NOik1waXGhvjUAfZWPP_gR_kVoUT</recordid><startdate>1982</startdate><enddate>1982</enddate><creator>Dwivedi, Chandra M.</creator><creator>Plante, Laurence T.</creator><creator>Kisliuk, Roy L.</creator><creator>Pastore, Edward J.</creator><creator>Verma, Surendra P.</creator><creator>Wallach, Donald F.H.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1982</creationdate><title>Interaction of the carboxamide of NADPH with Lactobacillus casei dihydrofolate reductase</title><author>Dwivedi, Chandra M. ; Plante, Laurence T. ; Kisliuk, Roy L. ; Pastore, Edward J. ; Verma, Surendra P. ; Wallach, Donald F.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-da07ab8a02e5cc754b425ab696e0c61521f037ffa8839e666ec9ec548aba11e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Lactobacillus casei - enzymology</topic><topic>Methotrexate - metabolism</topic><topic>NADP - metabolism</topic><topic>Spectrum Analysis, Raman</topic><topic>Tetrahydrofolate Dehydrogenase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dwivedi, Chandra M.</creatorcontrib><creatorcontrib>Plante, Laurence T.</creatorcontrib><creatorcontrib>Kisliuk, Roy L.</creatorcontrib><creatorcontrib>Pastore, Edward J.</creatorcontrib><creatorcontrib>Verma, Surendra P.</creatorcontrib><creatorcontrib>Wallach, Donald F.H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dwivedi, Chandra M.</au><au>Plante, Laurence T.</au><au>Kisliuk, Roy L.</au><au>Pastore, Edward J.</au><au>Verma, Surendra P.</au><au>Wallach, Donald F.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of the carboxamide of NADPH with Lactobacillus casei dihydrofolate reductase</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1982</date><risdate>1982</risdate><volume>213</volume><issue>1</issue><spage>338</spage><epage>340</epage><pages>338-340</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>Dihydrofolate reductase from
Lactobacillus casei and its complexes with NADPH and methotrexate yield well-resolved Raman spectra. The 1685-cm
−1 Raman band assigned to the carboxamide of NADPH persists in the NADPH-enzyme binary complex but is absent from the NADPH-methotrexate-enzyme ternary complex. This is ascribed to stabilization of the polarized form of the carboxamide by H bonding to the NH and CO groups of Ala 6 and Ile 13 of the peptide backbone.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6800305</pmid><doi>10.1016/0003-9861(82)90471-4</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Archives of biochemistry and biophysics, 1982, Vol.213 (1), p.338-340 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74003609 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Lactobacillus casei - enzymology Methotrexate - metabolism NADP - metabolism Spectrum Analysis, Raman Tetrahydrofolate Dehydrogenase - metabolism |
| title | Interaction of the carboxamide of NADPH with Lactobacillus casei dihydrofolate reductase |
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