[3H]serotonin release: An improved method to measure mast cell degranulation
A method based on the release of tritium-labelled serotonin by activated mast cells in rodents is described. Mast cells incorporate labelled serotonin selectively and release the label after activation by non-specific stimulators (compound 48/80, polymyxin B sulphate, ATP, bovine chymotrypsin and L-...
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| Veröffentlicht in: | Journal of immunological methods 1978-01, Vol.21 (1-2), p.65-77 |
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| container_title | Journal of immunological methods |
| container_volume | 21 |
| creator | Mazingue, Christine Dessaint, Jean-Paul Capron, André |
| description | A method based on the release of tritium-labelled serotonin by activated mast cells in rodents is described. Mast cells incorporate labelled serotonin selectively and release the label after activation by non-specific stimulators (compound 48/80, polymyxin B sulphate, ATP, bovine chymotrypsin and L-α-lysophosphatidylcholine) or anaphylactic antibody and the corresponding antigen. These two types of activation were investigated in comparison with the toluidine blue microscopic rat mast cell degranulation test, and a methodological study of the release of [3H]serotonin in described. The measurement of labelled serotonin release provides a simple and quick assay of mast cell degranulation compared to the time required for the classical rat mast cell degranulation technique and achieves a greater sensitivity. |
| doi_str_mv | 10.1016/0022-1759(78)90224-7 |
| format | Article |
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The measurement of labelled serotonin release provides a simple and quick assay of mast cell degranulation compared to the time required for the classical rat mast cell degranulation technique and achieves a greater sensitivity.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(78)90224-7</identifier><identifier>PMID: 96185</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antigen-Antibody Reactions ; Ascitic Fluid - immunology ; Coumarins - pharmacology ; Cromolyn Sodium - pharmacology ; Cytoplasmic Granules - immunology ; Dose-Response Relationship, Immunologic ; Female ; Mast Cells - immunology ; Mice ; Mice, Inbred CBA ; p-Methoxy-N-methylphenethylamine - pharmacology ; Papaverine - pharmacology ; Rats ; Serotonin - metabolism ; Tritium</subject><ispartof>Journal of immunological methods, 1978-01, Vol.21 (1-2), p.65-77</ispartof><rights>1978</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-3470264e58604e142dd97dab02806d6a801ac4e2eed5979c9dca22e078e2552b3</citedby><cites>FETCH-LOGICAL-c421t-3470264e58604e142dd97dab02806d6a801ac4e2eed5979c9dca22e078e2552b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-1759(78)90224-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/96185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mazingue, Christine</creatorcontrib><creatorcontrib>Dessaint, Jean-Paul</creatorcontrib><creatorcontrib>Capron, André</creatorcontrib><title>[3H]serotonin release: An improved method to measure mast cell degranulation</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>A method based on the release of tritium-labelled serotonin by activated mast cells in rodents is described. Mast cells incorporate labelled serotonin selectively and release the label after activation by non-specific stimulators (compound 48/80, polymyxin B sulphate, ATP, bovine chymotrypsin and L-α-lysophosphatidylcholine) or anaphylactic antibody and the corresponding antigen. These two types of activation were investigated in comparison with the toluidine blue microscopic rat mast cell degranulation test, and a methodological study of the release of [3H]serotonin in described. The measurement of labelled serotonin release provides a simple and quick assay of mast cell degranulation compared to the time required for the classical rat mast cell degranulation technique and achieves a greater sensitivity.</description><subject>Animals</subject><subject>Antigen-Antibody Reactions</subject><subject>Ascitic Fluid - immunology</subject><subject>Coumarins - pharmacology</subject><subject>Cromolyn Sodium - pharmacology</subject><subject>Cytoplasmic Granules - immunology</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Female</subject><subject>Mast Cells - immunology</subject><subject>Mice</subject><subject>Mice, Inbred CBA</subject><subject>p-Methoxy-N-methylphenethylamine - pharmacology</subject><subject>Papaverine - pharmacology</subject><subject>Rats</subject><subject>Serotonin - metabolism</subject><subject>Tritium</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFLAzEQhYNYtFZ_gR72JHpYnaTZTdaDUIpaoeBFTyIhTaYa2d3UZLfgv3e3LT16mhnmzePNR8gFhRsKNL8FYCylIiuuhLwuuoGn4oAMqRQsFQVkh2S4lxyTkxi_AYBCDkdkUORUZkMyfx_PPiIG3_ja1UnAEnXEu2RSJ65aBb9Gm1TYfHmbNL7rdGwDJpWOTWKwLBOLn0HXbakb5-tTMljqMuLZro7I2-PD63SWzl-enqeTeWo4o0065gJYzjGTOXCknFlbCKsXwCTkNtcSqDYcGaLNClGYwhrNGIKQyLKMLcYjcrn17QL-tBgbVbnYx9E1-jYqwfs3M9kJ-VZogo8x4FKtgqt0-FUUVI9Q9XxUz0cJqTYIlejOznf-7aJCuz_aMOu299stdi-uHQYVjcPaoHUBTaOsd__b_wGHgX9L</recordid><startdate>19780101</startdate><enddate>19780101</enddate><creator>Mazingue, Christine</creator><creator>Dessaint, Jean-Paul</creator><creator>Capron, André</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19780101</creationdate><title>[3H]serotonin release: An improved method to measure mast cell degranulation</title><author>Mazingue, Christine ; Dessaint, Jean-Paul ; Capron, André</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-3470264e58604e142dd97dab02806d6a801ac4e2eed5979c9dca22e078e2552b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Animals</topic><topic>Antigen-Antibody Reactions</topic><topic>Ascitic Fluid - immunology</topic><topic>Coumarins - pharmacology</topic><topic>Cromolyn Sodium - pharmacology</topic><topic>Cytoplasmic Granules - immunology</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>Female</topic><topic>Mast Cells - immunology</topic><topic>Mice</topic><topic>Mice, Inbred CBA</topic><topic>p-Methoxy-N-methylphenethylamine - pharmacology</topic><topic>Papaverine - pharmacology</topic><topic>Rats</topic><topic>Serotonin - metabolism</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mazingue, Christine</creatorcontrib><creatorcontrib>Dessaint, Jean-Paul</creatorcontrib><creatorcontrib>Capron, André</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mazingue, Christine</au><au>Dessaint, Jean-Paul</au><au>Capron, André</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>[3H]serotonin release: An improved method to measure mast cell degranulation</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1978-01-01</date><risdate>1978</risdate><volume>21</volume><issue>1-2</issue><spage>65</spage><epage>77</epage><pages>65-77</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>A method based on the release of tritium-labelled serotonin by activated mast cells in rodents is described. 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| ispartof | Journal of immunological methods, 1978-01, Vol.21 (1-2), p.65-77 |
| issn | 0022-1759 1872-7905 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Antigen-Antibody Reactions Ascitic Fluid - immunology Coumarins - pharmacology Cromolyn Sodium - pharmacology Cytoplasmic Granules - immunology Dose-Response Relationship, Immunologic Female Mast Cells - immunology Mice Mice, Inbred CBA p-Methoxy-N-methylphenethylamine - pharmacology Papaverine - pharmacology Rats Serotonin - metabolism Tritium |
| title | [3H]serotonin release: An improved method to measure mast cell degranulation |
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