Localization of some frog virus 3 structural polypeptides
The localization of several frog virus 3 structural polypeptides has been established by stripping the different layers of the virion with controlled degradations using Brij, NP 40, and Pronase. The degradation products were further separated on sucrose gradients and characterized by electron micros...
Gespeichert in:
| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1982-01, Vol.116 (2), p.635-640 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 640 |
|---|---|
| container_issue | 2 |
| container_start_page | 635 |
| container_title | Virology (New York, N.Y.) |
| container_volume | 116 |
| creator | Tripier-Darcy, Françoise Braunwald, Jacqueline Kirn, André |
| description | The localization of several frog virus 3 structural polypeptides has been established by stripping the different layers of the virion with controlled degradations using Brij, NP 40, and Pronase. The degradation products were further separated on sucrose gradients and characterized by electron microscopy and SDS-gel electrophoresis. This study allowed us to determine the radial distribution of the following viral polypeptides (VPs): VP 58 in the viral envelope, VP 48 in the capsid subunits, and VPs 44 and 63, beneath the icosahedral lattice, probably associated with the lipids. Additional information is also provided on FV
3 ultrastructure from the study of the morphology of different types of well-characterized subviral particles. |
| doi_str_mv | 10.1016/0042-6822(82)90155-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73996974</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0042682282901556</els_id><sourcerecordid>15443469</sourcerecordid><originalsourceid>FETCH-LOGICAL-c419t-e852631bbe052b1f532cfc79311f70f9598e31dc672173571858f70136f4a5923</originalsourceid><addsrcrecordid>eNqNUU1rGzEUFKXBcdz-gwb2VJrDJnr61iUQQr7AkEt6FmvtU1FZW1tpN-D8-q5r42OT0-MxM294M4R8A3oJFNQVpYLVyjD2w7ALS0HKWn0ic6BW1ZQL-EzmR8opOSvlN512remMzJTV2nI5J3aZfNPFt2aIaVOlUJW0xirk9Kt6jXksFa_KkEc_jLnpqj512x77IbZYvpCT0HQFvx7mgvy8v3u5fayXzw9PtzfL2guwQ41GMsVhtUIq2QqC5MwHP5kDBE2DldYgh9YrzUBzqcFIMwHAVRCNtIwvyPf93T6nPyOWwa1j8dh1zQbTWJzm1k7viHeJIIXgQtkPELkRxuysxZ7ocyolY3B9jusmbx1Qt-vA7QJ2u4CdYe5fB05NsvPD_XG1xvYoOoQ-4dd7HKfYXiNmV3zEjcc2ZvSDa1P8v8FfZ2STZA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15384882</pqid></control><display><type>article</type><title>Localization of some frog virus 3 structural polypeptides</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Tripier-Darcy, Françoise ; Braunwald, Jacqueline ; Kirn, André</creator><creatorcontrib>Tripier-Darcy, Françoise ; Braunwald, Jacqueline ; Kirn, André</creatorcontrib><description>The localization of several frog virus 3 structural polypeptides has been established by stripping the different layers of the virion with controlled degradations using Brij, NP 40, and Pronase. The degradation products were further separated on sucrose gradients and characterized by electron microscopy and SDS-gel electrophoresis. This study allowed us to determine the radial distribution of the following viral polypeptides (VPs): VP 58 in the viral envelope, VP 48 in the capsid subunits, and VPs 44 and 63, beneath the icosahedral lattice, probably associated with the lipids. Additional information is also provided on FV
3 ultrastructure from the study of the morphology of different types of well-characterized subviral particles.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(82)90155-6</identifier><identifier>PMID: 6977935</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Capsid - isolation & purification ; Centrifugation, Density Gradient ; Detergents - pharmacology ; Electrophoresis, Polyacrylamide Gel ; frog virus 3 ; Iridoviridae - metabolism ; Iridoviridae - ultrastructure ; Pronase - pharmacology ; Rana pipiens ; Viral Envelope Proteins ; Viral Proteins - analysis ; Viral Proteins - isolation & purification</subject><ispartof>Virology (New York, N.Y.), 1982-01, Vol.116 (2), p.635-640</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-e852631bbe052b1f532cfc79311f70f9598e31dc672173571858f70136f4a5923</citedby><cites>FETCH-LOGICAL-c419t-e852631bbe052b1f532cfc79311f70f9598e31dc672173571858f70136f4a5923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0042-6822(82)90155-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6977935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tripier-Darcy, Françoise</creatorcontrib><creatorcontrib>Braunwald, Jacqueline</creatorcontrib><creatorcontrib>Kirn, André</creatorcontrib><title>Localization of some frog virus 3 structural polypeptides</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The localization of several frog virus 3 structural polypeptides has been established by stripping the different layers of the virion with controlled degradations using Brij, NP 40, and Pronase. The degradation products were further separated on sucrose gradients and characterized by electron microscopy and SDS-gel electrophoresis. This study allowed us to determine the radial distribution of the following viral polypeptides (VPs): VP 58 in the viral envelope, VP 48 in the capsid subunits, and VPs 44 and 63, beneath the icosahedral lattice, probably associated with the lipids. Additional information is also provided on FV
3 ultrastructure from the study of the morphology of different types of well-characterized subviral particles.</description><subject>Animals</subject><subject>Capsid - isolation & purification</subject><subject>Centrifugation, Density Gradient</subject><subject>Detergents - pharmacology</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>frog virus 3</subject><subject>Iridoviridae - metabolism</subject><subject>Iridoviridae - ultrastructure</subject><subject>Pronase - pharmacology</subject><subject>Rana pipiens</subject><subject>Viral Envelope Proteins</subject><subject>Viral Proteins - analysis</subject><subject>Viral Proteins - isolation & purification</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUU1rGzEUFKXBcdz-gwb2VJrDJnr61iUQQr7AkEt6FmvtU1FZW1tpN-D8-q5r42OT0-MxM294M4R8A3oJFNQVpYLVyjD2w7ALS0HKWn0ic6BW1ZQL-EzmR8opOSvlN512remMzJTV2nI5J3aZfNPFt2aIaVOlUJW0xirk9Kt6jXksFa_KkEc_jLnpqj512x77IbZYvpCT0HQFvx7mgvy8v3u5fayXzw9PtzfL2guwQ41GMsVhtUIq2QqC5MwHP5kDBE2DldYgh9YrzUBzqcFIMwHAVRCNtIwvyPf93T6nPyOWwa1j8dh1zQbTWJzm1k7viHeJIIXgQtkPELkRxuysxZ7ocyolY3B9jusmbx1Qt-vA7QJ2u4CdYe5fB05NsvPD_XG1xvYoOoQ-4dd7HKfYXiNmV3zEjcc2ZvSDa1P8v8FfZ2STZA</recordid><startdate>19820101</startdate><enddate>19820101</enddate><creator>Tripier-Darcy, Françoise</creator><creator>Braunwald, Jacqueline</creator><creator>Kirn, André</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19820101</creationdate><title>Localization of some frog virus 3 structural polypeptides</title><author>Tripier-Darcy, Françoise ; Braunwald, Jacqueline ; Kirn, André</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-e852631bbe052b1f532cfc79311f70f9598e31dc672173571858f70136f4a5923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Capsid - isolation & purification</topic><topic>Centrifugation, Density Gradient</topic><topic>Detergents - pharmacology</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>frog virus 3</topic><topic>Iridoviridae - metabolism</topic><topic>Iridoviridae - ultrastructure</topic><topic>Pronase - pharmacology</topic><topic>Rana pipiens</topic><topic>Viral Envelope Proteins</topic><topic>Viral Proteins - analysis</topic><topic>Viral Proteins - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tripier-Darcy, Françoise</creatorcontrib><creatorcontrib>Braunwald, Jacqueline</creatorcontrib><creatorcontrib>Kirn, André</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tripier-Darcy, Françoise</au><au>Braunwald, Jacqueline</au><au>Kirn, André</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of some frog virus 3 structural polypeptides</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1982-01-01</date><risdate>1982</risdate><volume>116</volume><issue>2</issue><spage>635</spage><epage>640</epage><pages>635-640</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>The localization of several frog virus 3 structural polypeptides has been established by stripping the different layers of the virion with controlled degradations using Brij, NP 40, and Pronase. The degradation products were further separated on sucrose gradients and characterized by electron microscopy and SDS-gel electrophoresis. This study allowed us to determine the radial distribution of the following viral polypeptides (VPs): VP 58 in the viral envelope, VP 48 in the capsid subunits, and VPs 44 and 63, beneath the icosahedral lattice, probably associated with the lipids. Additional information is also provided on FV
3 ultrastructure from the study of the morphology of different types of well-characterized subviral particles.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6977935</pmid><doi>10.1016/0042-6822(82)90155-6</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1982-01, Vol.116 (2), p.635-640 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73996974 |
| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Capsid - isolation & purification Centrifugation, Density Gradient Detergents - pharmacology Electrophoresis, Polyacrylamide Gel frog virus 3 Iridoviridae - metabolism Iridoviridae - ultrastructure Pronase - pharmacology Rana pipiens Viral Envelope Proteins Viral Proteins - analysis Viral Proteins - isolation & purification |
| title | Localization of some frog virus 3 structural polypeptides |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T02%3A40%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Localization%20of%20some%20frog%20virus%203%20structural%20polypeptides&rft.jtitle=Virology%20(New%20York,%20N.Y.)&rft.au=Tripier-Darcy,%20Fran%C3%A7oise&rft.date=1982-01-01&rft.volume=116&rft.issue=2&rft.spage=635&rft.epage=640&rft.pages=635-640&rft.issn=0042-6822&rft.eissn=1096-0341&rft_id=info:doi/10.1016/0042-6822(82)90155-6&rft_dat=%3Cproquest_cross%3E15443469%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15384882&rft_id=info:pmid/6977935&rft_els_id=0042682282901556&rfr_iscdi=true |