The Kinetics of Acetylcholine Turnover in a Resting Cholinergic Nerve Terminal and the Magnitude of the Cytoplasmic Compartment
: The magnitude of the cytosolic pool of acetylcholine in the cholinergic electromotor nerve terminals of Torpedo marmorata has been calculated to be 22 ± 3% of the total by comparing the isotopic ratio of acetylcholine with that of choline when slices of electric organ were incubated with 10 μM deu...
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| Veröffentlicht in: | Journal of neurochemistry 1982-05, Vol.38 (5), p.1187-1191 |
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| container_end_page | 1191 |
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| container_issue | 5 |
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| container_title | Journal of neurochemistry |
| container_volume | 38 |
| creator | Weiler, M. Roed, I. S. Whittaker, V. P. |
| description | : The magnitude of the cytosolic pool of acetylcholine in the cholinergic electromotor nerve terminals of Torpedo marmorata has been calculated to be 22 ± 3% of the total by comparing the isotopic ratio of acetylcholine with that of choline when slices of electric organ were incubated with 10 μM deuterated choline. The calculation is based on a two‐compartment model that assumes the presence, in unstimulated tissue, of a vesicular pool of acetylcholine that does not exchange, under resting conditions, with a second cytosolic pool; the latter, by contrast, is subject to ‘futile recycling’ and comes into isotopic equilibrium with the tissue choline pool. |
| doi_str_mv | 10.1111/j.1471-4159.1982.tb07889.x |
| format | Article |
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The calculation is based on a two‐compartment model that assumes the presence, in unstimulated tissue, of a vesicular pool of acetylcholine that does not exchange, under resting conditions, with a second cytosolic pool; the latter, by contrast, is subject to ‘futile recycling’ and comes into isotopic equilibrium with the tissue choline pool.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1982.tb07889.x</identifier><identifier>PMID: 7062045</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acetylcholine ; Acetylcholine - metabolism ; Animals ; Cell Compartmentation ; Choline - metabolism ; Cytoplasm - metabolism ; Cytosolic compartment ; Electric organ ; Electric Organ - metabolism ; Female ; Kinetics ; Motor Neurons - metabolism ; Receptors, Cholinergic - metabolism ; Torpedo</subject><ispartof>Journal of neurochemistry, 1982-05, Vol.38 (5), p.1187-1191</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3707-5c3568e077d0e6fcb831682f405142c65edab825c490d52917035a774808525e3</citedby><cites>FETCH-LOGICAL-c3707-5c3568e077d0e6fcb831682f405142c65edab825c490d52917035a774808525e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.1982.tb07889.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.1982.tb07889.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7062045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weiler, M.</creatorcontrib><creatorcontrib>Roed, I. S.</creatorcontrib><creatorcontrib>Whittaker, V. P.</creatorcontrib><title>The Kinetics of Acetylcholine Turnover in a Resting Cholinergic Nerve Terminal and the Magnitude of the Cytoplasmic Compartment</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: The magnitude of the cytosolic pool of acetylcholine in the cholinergic electromotor nerve terminals of Torpedo marmorata has been calculated to be 22 ± 3% of the total by comparing the isotopic ratio of acetylcholine with that of choline when slices of electric organ were incubated with 10 μM deuterated choline. The calculation is based on a two‐compartment model that assumes the presence, in unstimulated tissue, of a vesicular pool of acetylcholine that does not exchange, under resting conditions, with a second cytosolic pool; the latter, by contrast, is subject to ‘futile recycling’ and comes into isotopic equilibrium with the tissue choline pool.</description><subject>Acetylcholine</subject><subject>Acetylcholine - metabolism</subject><subject>Animals</subject><subject>Cell Compartmentation</subject><subject>Choline - metabolism</subject><subject>Cytoplasm - metabolism</subject><subject>Cytosolic compartment</subject><subject>Electric organ</subject><subject>Electric Organ - metabolism</subject><subject>Female</subject><subject>Kinetics</subject><subject>Motor Neurons - metabolism</subject><subject>Receptors, Cholinergic - metabolism</subject><subject>Torpedo</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE1v1DAQhi0EKkvhJyBZHLgljB07drigKiofpbQSWs6W15lsvUqcxU7a7ql_nUS76p25jDTvh-WHkA8McjbPp13OhGKZYLLKWaV5Pm5AaV3ljy_I6ll6SVYAnGcFCP6avElpB8BKUbIzcqag5CDkijyt75D-9AFH7xIdWnrhcDx07m7o5iNdTzEM9xipD9TS35hGH7a0Pqpx6x29wXg_-zD2PtiO2tDQca78ZbfBj1ODS-dyqA_jsO9s6udMPfR7G8cew_iWvGptl_DdaZ-TP18v1_X37Pr224_64jpzhQKVSVfIUiMo1QCWrdvogpWatwIkE9yVEhu70Vw6UUEjecUUFNIqJTRoySUW5-TjsXcfh7_T_A_T--Sw62zAYUpGFVUFsoTZ-PlodHFIKWJr9tH3Nh4MA7PQNzuzIDYLYrPQNyf65nEOvz-9Mm16bJ6jJ9yz_uWoP_gOD__RbK5uasa0Kv4BcmuVYg</recordid><startdate>198205</startdate><enddate>198205</enddate><creator>Weiler, M.</creator><creator>Roed, I. S.</creator><creator>Whittaker, V. P.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198205</creationdate><title>The Kinetics of Acetylcholine Turnover in a Resting Cholinergic Nerve Terminal and the Magnitude of the Cytoplasmic Compartment</title><author>Weiler, M. ; Roed, I. S. ; Whittaker, V. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3707-5c3568e077d0e6fcb831682f405142c65edab825c490d52917035a774808525e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Acetylcholine</topic><topic>Acetylcholine - metabolism</topic><topic>Animals</topic><topic>Cell Compartmentation</topic><topic>Choline - metabolism</topic><topic>Cytoplasm - metabolism</topic><topic>Cytosolic compartment</topic><topic>Electric organ</topic><topic>Electric Organ - metabolism</topic><topic>Female</topic><topic>Kinetics</topic><topic>Motor Neurons - metabolism</topic><topic>Receptors, Cholinergic - metabolism</topic><topic>Torpedo</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weiler, M.</creatorcontrib><creatorcontrib>Roed, I. S.</creatorcontrib><creatorcontrib>Whittaker, V. P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weiler, M.</au><au>Roed, I. S.</au><au>Whittaker, V. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Kinetics of Acetylcholine Turnover in a Resting Cholinergic Nerve Terminal and the Magnitude of the Cytoplasmic Compartment</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1982-05</date><risdate>1982</risdate><volume>38</volume><issue>5</issue><spage>1187</spage><epage>1191</epage><pages>1187-1191</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><abstract>: The magnitude of the cytosolic pool of acetylcholine in the cholinergic electromotor nerve terminals of Torpedo marmorata has been calculated to be 22 ± 3% of the total by comparing the isotopic ratio of acetylcholine with that of choline when slices of electric organ were incubated with 10 μM deuterated choline. The calculation is based on a two‐compartment model that assumes the presence, in unstimulated tissue, of a vesicular pool of acetylcholine that does not exchange, under resting conditions, with a second cytosolic pool; the latter, by contrast, is subject to ‘futile recycling’ and comes into isotopic equilibrium with the tissue choline pool.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7062045</pmid><doi>10.1111/j.1471-4159.1982.tb07889.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of neurochemistry, 1982-05, Vol.38 (5), p.1187-1191 |
| issn | 0022-3042 1471-4159 |
| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Acetylcholine Acetylcholine - metabolism Animals Cell Compartmentation Choline - metabolism Cytoplasm - metabolism Cytosolic compartment Electric organ Electric Organ - metabolism Female Kinetics Motor Neurons - metabolism Receptors, Cholinergic - metabolism Torpedo |
| title | The Kinetics of Acetylcholine Turnover in a Resting Cholinergic Nerve Terminal and the Magnitude of the Cytoplasmic Compartment |
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