Elevated level of enzymatic DNA methylation in cells treated with 1-beta-D-arabinofuranosylcytosine
When mouse P815 mastocytoma cells are cultured in the presence of 1-beta-D-arabinofuranosylcytosine (ara-C), a dose-dependent inhibition of cell proliferation and DNA synthesis can be observed. The DNA synthesized in the presence of ara-C is more highly enzymatically methylated than that of controls...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1982-04, Vol.42 (4), p.1537-1540 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | Boehm, T L Drahovsky, D |
| description | When mouse P815 mastocytoma cells are cultured in the presence of 1-beta-D-arabinofuranosylcytosine (ara-C), a dose-dependent inhibition of cell proliferation and DNA synthesis can be observed. The DNA synthesized in the presence of ara-C is more highly enzymatically methylated than that of controls as measured by conversion of incorporated [14C]deoxycytidine-derived radioactivity into 5-methylcytosines. Analysis of DNAs by means of the bacterial restriction enzyme HpaII and its isoschizomer MspI revealed an aberrant DNA methylation pattern after ara-C treatment. The hypermethylation of DNA persisted also in the cell cycle following the treatment in the absence of ara-C. No such changes in DNA methylation patterns following ara-C treatment were observed in P815/ara-C, a subclone of P815 cells resistant to ara-C. |
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The DNA synthesized in the presence of ara-C is more highly enzymatically methylated than that of controls as measured by conversion of incorporated [14C]deoxycytidine-derived radioactivity into 5-methylcytosines. Analysis of DNAs by means of the bacterial restriction enzyme HpaII and its isoschizomer MspI revealed an aberrant DNA methylation pattern after ara-C treatment. The hypermethylation of DNA persisted also in the cell cycle following the treatment in the absence of ara-C. 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The DNA synthesized in the presence of ara-C is more highly enzymatically methylated than that of controls as measured by conversion of incorporated [14C]deoxycytidine-derived radioactivity into 5-methylcytosines. Analysis of DNAs by means of the bacterial restriction enzyme HpaII and its isoschizomer MspI revealed an aberrant DNA methylation pattern after ara-C treatment. The hypermethylation of DNA persisted also in the cell cycle following the treatment in the absence of ara-C. 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The DNA synthesized in the presence of ara-C is more highly enzymatically methylated than that of controls as measured by conversion of incorporated [14C]deoxycytidine-derived radioactivity into 5-methylcytosines. Analysis of DNAs by means of the bacterial restriction enzyme HpaII and its isoschizomer MspI revealed an aberrant DNA methylation pattern after ara-C treatment. The hypermethylation of DNA persisted also in the cell cycle following the treatment in the absence of ara-C. No such changes in DNA methylation patterns following ara-C treatment were observed in P815/ara-C, a subclone of P815 cells resistant to ara-C.</abstract><cop>United States</cop><pmid>7060025</pmid><tpages>4</tpages></addata></record> |
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| ispartof | Cancer research (Chicago, Ill.), 1982-04, Vol.42 (4), p.1537-1540 |
| issn | 0008-5472 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73988078 |
| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Cytarabine - pharmacology DNA (Cytosine-5-)-Methyltransferases DNA - metabolism Methylation Mice Mice, Inbred Strains |
| title | Elevated level of enzymatic DNA methylation in cells treated with 1-beta-D-arabinofuranosylcytosine |
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