A sandwich method of enzyme-immunoassay. II. Quantification of rheumatoid factor
A sandwich enzyme-immunoassay (EIA) has been applied to the determination of the rheumatoid factor (RF). This non-competitive assay comprises 3 steps: 1) the RF to be assayed is extracted from the biological medium by an immunosorbent of aggregated IgG linked to cellulose; 2) the solid phase is then...
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| Veröffentlicht in: | Journal of immunological methods 1978, Vol.20, p.25-34 |
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| container_title | Journal of immunological methods |
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| creator | Maiolini, R. Ferrua, B. Quaranta, J.F. Pinoteau, A. Euller, L. Ziegler, G. Masseyeff, R. |
| description | A sandwich enzyme-immunoassay (EIA) has been applied to the determination of the rheumatoid factor (RF). This non-competitive assay comprises 3 steps: 1) the RF to be assayed is extracted from the biological medium by an immunosorbent of aggregated IgG linked to cellulose; 2) the solid phase is then incubated with the enzyme-labeled aggregated IgG; 3) the enzymatic activity of the immunosorbent is then measured with a suitable chromogenic reagent. This activity is a direct function of the amount of RF to be assayed.
This assay gave reproducible results in the range 0.5–50.0 IU/ml. A good agreement was obtained between the EIA and the Waaler-Rose test but no correlation was obtained with the latex slide-test.
This assay permits a quantitation of RF with a good reproducibility (coefficient of variation in the range of 10% for moderately elevated values) and thus allows a closer follow-up of patients. The results do not depend on the interpretation of the technician performing the test, which can be easily automated. Finally, it may detect some RF devoid of agglutinating activity. |
| doi_str_mv | 10.1016/0022-1759(78)90241-7 |
| format | Article |
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This assay gave reproducible results in the range 0.5–50.0 IU/ml. A good agreement was obtained between the EIA and the Waaler-Rose test but no correlation was obtained with the latex slide-test.
This assay permits a quantitation of RF with a good reproducibility (coefficient of variation in the range of 10% for moderately elevated values) and thus allows a closer follow-up of patients. The results do not depend on the interpretation of the technician performing the test, which can be easily automated. Finally, it may detect some RF devoid of agglutinating activity.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(78)90241-7</identifier><identifier>PMID: 649961</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibody Specificity ; Enzyme-Linked Immunosorbent Assay - methods ; Humans ; Immunoglobulin G ; Quality Control ; Rabbits ; Rheumatoid Factor</subject><ispartof>Journal of immunological methods, 1978, Vol.20, p.25-34</ispartof><rights>1978</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c271t-6ed7db7de33be125b281e367ebc88442d5252f4101dfec3aef830c792ee62a923</citedby><cites>FETCH-LOGICAL-c271t-6ed7db7de33be125b281e367ebc88442d5252f4101dfec3aef830c792ee62a923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-1759(78)90241-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,4023,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/649961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maiolini, R.</creatorcontrib><creatorcontrib>Ferrua, B.</creatorcontrib><creatorcontrib>Quaranta, J.F.</creatorcontrib><creatorcontrib>Pinoteau, A.</creatorcontrib><creatorcontrib>Euller, L.</creatorcontrib><creatorcontrib>Ziegler, G.</creatorcontrib><creatorcontrib>Masseyeff, R.</creatorcontrib><title>A sandwich method of enzyme-immunoassay. II. Quantification of rheumatoid factor</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>A sandwich enzyme-immunoassay (EIA) has been applied to the determination of the rheumatoid factor (RF). This non-competitive assay comprises 3 steps: 1) the RF to be assayed is extracted from the biological medium by an immunosorbent of aggregated IgG linked to cellulose; 2) the solid phase is then incubated with the enzyme-labeled aggregated IgG; 3) the enzymatic activity of the immunosorbent is then measured with a suitable chromogenic reagent. This activity is a direct function of the amount of RF to be assayed.
This assay gave reproducible results in the range 0.5–50.0 IU/ml. A good agreement was obtained between the EIA and the Waaler-Rose test but no correlation was obtained with the latex slide-test.
This assay permits a quantitation of RF with a good reproducibility (coefficient of variation in the range of 10% for moderately elevated values) and thus allows a closer follow-up of patients. The results do not depend on the interpretation of the technician performing the test, which can be easily automated. Finally, it may detect some RF devoid of agglutinating activity.</description><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Humans</subject><subject>Immunoglobulin G</subject><subject>Quality Control</subject><subject>Rabbits</subject><subject>Rheumatoid Factor</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoN4q9U36GJWooupSeaSyUYoxUuhoIKuQyY5oZFmosmMUp_eGVu6lLM4i_8C_4fQhOApwaS8wZjSlLCCX7HqmmOak5QdoBGpGE0Zx8UhGu0tp-gsxneMMcElPkHHZc55SUboeZZE2ehvq1aJg3bldeJNAs3PxkFqnesaL2OUm2myWEyTl042rTVWydb6ZnCGFXROtt7qxEjV-nCOjoxcR7jY_TF6u797nT-my6eHxXy2TBVlpE1L0EzXTEOW1UBoUdOKQFYyqFVV5TnVBS2oyfud2oDKJJgqw4pxClBSyWk2Rpfb3o_gPzuIrXA2KlivZQO-i4JlnPU3GPOtUQUfYwAjPoJ1MmwEwWLgKAZIYoAkWCX-OPbpMZrs-rvagd6HtuB6-XYrQ7_xy0IQUVloFGgbQLVCe_t__y9J_YHW</recordid><startdate>1978</startdate><enddate>1978</enddate><creator>Maiolini, R.</creator><creator>Ferrua, B.</creator><creator>Quaranta, J.F.</creator><creator>Pinoteau, A.</creator><creator>Euller, L.</creator><creator>Ziegler, G.</creator><creator>Masseyeff, R.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1978</creationdate><title>A sandwich method of enzyme-immunoassay. II. Quantification of rheumatoid factor</title><author>Maiolini, R. ; Ferrua, B. ; Quaranta, J.F. ; Pinoteau, A. ; Euller, L. ; Ziegler, G. ; Masseyeff, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c271t-6ed7db7de33be125b281e367ebc88442d5252f4101dfec3aef830c792ee62a923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Humans</topic><topic>Immunoglobulin G</topic><topic>Quality Control</topic><topic>Rabbits</topic><topic>Rheumatoid Factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maiolini, R.</creatorcontrib><creatorcontrib>Ferrua, B.</creatorcontrib><creatorcontrib>Quaranta, J.F.</creatorcontrib><creatorcontrib>Pinoteau, A.</creatorcontrib><creatorcontrib>Euller, L.</creatorcontrib><creatorcontrib>Ziegler, G.</creatorcontrib><creatorcontrib>Masseyeff, R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maiolini, R.</au><au>Ferrua, B.</au><au>Quaranta, J.F.</au><au>Pinoteau, A.</au><au>Euller, L.</au><au>Ziegler, G.</au><au>Masseyeff, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sandwich method of enzyme-immunoassay. II. Quantification of rheumatoid factor</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1978</date><risdate>1978</risdate><volume>20</volume><spage>25</spage><epage>34</epage><pages>25-34</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>A sandwich enzyme-immunoassay (EIA) has been applied to the determination of the rheumatoid factor (RF). This non-competitive assay comprises 3 steps: 1) the RF to be assayed is extracted from the biological medium by an immunosorbent of aggregated IgG linked to cellulose; 2) the solid phase is then incubated with the enzyme-labeled aggregated IgG; 3) the enzymatic activity of the immunosorbent is then measured with a suitable chromogenic reagent. This activity is a direct function of the amount of RF to be assayed.
This assay gave reproducible results in the range 0.5–50.0 IU/ml. A good agreement was obtained between the EIA and the Waaler-Rose test but no correlation was obtained with the latex slide-test.
This assay permits a quantitation of RF with a good reproducibility (coefficient of variation in the range of 10% for moderately elevated values) and thus allows a closer follow-up of patients. The results do not depend on the interpretation of the technician performing the test, which can be easily automated. Finally, it may detect some RF devoid of agglutinating activity.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>649961</pmid><doi>10.1016/0022-1759(78)90241-7</doi><tpages>10</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1978, Vol.20, p.25-34 |
| issn | 0022-1759 1872-7905 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Antibody Specificity Enzyme-Linked Immunosorbent Assay - methods Humans Immunoglobulin G Quality Control Rabbits Rheumatoid Factor |
| title | A sandwich method of enzyme-immunoassay. II. Quantification of rheumatoid factor |
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