A combined assay for the rapid preliminary detection of structural retroviruses
This report describes the use of equilibrium gradients, RNA dependent DNA polymerase assays and electron microscopy (EM) in a combined assay for the rapid preliminary detection of intact retroviruses in crude preparations. Positive combined assays of platelets from preleukemic patients corresponded...
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| Veröffentlicht in: | Cancer letters 1978-01, Vol.4 (3), p.163-169 |
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| container_end_page | 169 |
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| container_issue | 3 |
| container_start_page | 163 |
| container_title | Cancer letters |
| container_volume | 4 |
| creator | Erlick, Barry J. Fuscaldo, Anthony A. West, William Fuscaldo, Kathryn Brodsky, Isadore |
| description | This report describes the use of equilibrium gradients, RNA dependent DNA polymerase assays and electron microscopy (EM) in a combined assay for the rapid preliminary detection of intact retroviruses in crude preparations. Positive combined assays of platelets from preleukemic patients corresponded with karyotypic abnormalities found in these patients. Reconstruction experiments with Rauscher Leukemia Virus added to buffer or disrupted mouse spleen demonstrated the ease of detecting 10
9 or greater particles/g crude tissue, and the effects of buffer or added protein. |
| doi_str_mv | 10.1016/S0304-3835(78)94182-4 |
| format | Article |
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9 or greater particles/g crude tissue, and the effects of buffer or added protein.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/S0304-3835(78)94182-4</identifier><identifier>PMID: 77185</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Blood Platelets - microbiology ; Humans ; Inclusion Bodies, Viral ; Leukemia, Experimental - microbiology ; Mice ; Microscopy, Electron ; Preleukemia - enzymology ; Preleukemia - microbiology ; Preleukemia - ultrastructure ; Rauscher Virus - isolation & purification ; Retroviridae - enzymology ; Retroviridae - isolation & purification ; RNA-Directed DNA Polymerase - analysis ; Spleen - microbiology</subject><ispartof>Cancer letters, 1978-01, Vol.4 (3), p.163-169</ispartof><rights>1978 Elsevier/North-Holland Scientific Publishers Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-2f233e8f6b87b2a327ed14ded507eff1a31d0dc541b219e6ec8dc7ab1c97165b3</citedby><cites>FETCH-LOGICAL-c358t-2f233e8f6b87b2a327ed14ded507eff1a31d0dc541b219e6ec8dc7ab1c97165b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-3835(78)94182-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/77185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Erlick, Barry J.</creatorcontrib><creatorcontrib>Fuscaldo, Anthony A.</creatorcontrib><creatorcontrib>West, William</creatorcontrib><creatorcontrib>Fuscaldo, Kathryn</creatorcontrib><creatorcontrib>Brodsky, Isadore</creatorcontrib><title>A combined assay for the rapid preliminary detection of structural retroviruses</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>This report describes the use of equilibrium gradients, RNA dependent DNA polymerase assays and electron microscopy (EM) in a combined assay for the rapid preliminary detection of intact retroviruses in crude preparations. Positive combined assays of platelets from preleukemic patients corresponded with karyotypic abnormalities found in these patients. Reconstruction experiments with Rauscher Leukemia Virus added to buffer or disrupted mouse spleen demonstrated the ease of detecting 10
9 or greater particles/g crude tissue, and the effects of buffer or added protein.</description><subject>Animals</subject><subject>Blood Platelets - microbiology</subject><subject>Humans</subject><subject>Inclusion Bodies, Viral</subject><subject>Leukemia, Experimental - microbiology</subject><subject>Mice</subject><subject>Microscopy, Electron</subject><subject>Preleukemia - enzymology</subject><subject>Preleukemia - microbiology</subject><subject>Preleukemia - ultrastructure</subject><subject>Rauscher Virus - isolation & purification</subject><subject>Retroviridae - enzymology</subject><subject>Retroviridae - isolation & purification</subject><subject>RNA-Directed DNA Polymerase - analysis</subject><subject>Spleen - microbiology</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUhoM46Dj6BCJkJbqoJk3TpCsZBm8wMAt1HdLkFCNtU5N2YN7ezoXZujqL_3L4P4RuKHmghOaPH4SRLGGS8Tsh74uMyjTJTtCUSpEmopDkFE2PlnN0EeMPIYRngp-hiRBU8ilazbHxTelasFjHqDe48gH334CD7pzFXYDaNa7VYYMt9GB651vsKxz7MJh-CLrGAfrg1y4MEeIlmlS6jnB1uDP09fL8uXhLlqvX98V8mRjGZZ-kVcoYyCovpShTzVIBlmYWLCcCqopqRi2xhme0TGkBORhpjdAlNYWgOS_ZDN3ue7vgfweIvWpcNFDXugU_RCVYIZhMyWjke6MJPsYAleqCa8Y5ihK1xah2GNWWkRJS7TCqbMxdHx4MZQP2mNpxG9WnvQrjxrWDoKJx0BqwLoyMlPXun_4_A-2Dag</recordid><startdate>19780101</startdate><enddate>19780101</enddate><creator>Erlick, Barry J.</creator><creator>Fuscaldo, Anthony A.</creator><creator>West, William</creator><creator>Fuscaldo, Kathryn</creator><creator>Brodsky, Isadore</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19780101</creationdate><title>A combined assay for the rapid preliminary detection of structural retroviruses</title><author>Erlick, Barry J. ; Fuscaldo, Anthony A. ; West, William ; Fuscaldo, Kathryn ; Brodsky, Isadore</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-2f233e8f6b87b2a327ed14ded507eff1a31d0dc541b219e6ec8dc7ab1c97165b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Animals</topic><topic>Blood Platelets - microbiology</topic><topic>Humans</topic><topic>Inclusion Bodies, Viral</topic><topic>Leukemia, Experimental - microbiology</topic><topic>Mice</topic><topic>Microscopy, Electron</topic><topic>Preleukemia - enzymology</topic><topic>Preleukemia - microbiology</topic><topic>Preleukemia - ultrastructure</topic><topic>Rauscher Virus - isolation & purification</topic><topic>Retroviridae - enzymology</topic><topic>Retroviridae - isolation & purification</topic><topic>RNA-Directed DNA Polymerase - analysis</topic><topic>Spleen - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Erlick, Barry J.</creatorcontrib><creatorcontrib>Fuscaldo, Anthony A.</creatorcontrib><creatorcontrib>West, William</creatorcontrib><creatorcontrib>Fuscaldo, Kathryn</creatorcontrib><creatorcontrib>Brodsky, Isadore</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Erlick, Barry J.</au><au>Fuscaldo, Anthony A.</au><au>West, William</au><au>Fuscaldo, Kathryn</au><au>Brodsky, Isadore</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A combined assay for the rapid preliminary detection of structural retroviruses</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>1978-01-01</date><risdate>1978</risdate><volume>4</volume><issue>3</issue><spage>163</spage><epage>169</epage><pages>163-169</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>This report describes the use of equilibrium gradients, RNA dependent DNA polymerase assays and electron microscopy (EM) in a combined assay for the rapid preliminary detection of intact retroviruses in crude preparations. Positive combined assays of platelets from preleukemic patients corresponded with karyotypic abnormalities found in these patients. Reconstruction experiments with Rauscher Leukemia Virus added to buffer or disrupted mouse spleen demonstrated the ease of detecting 10
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| fulltext | fulltext |
| identifier | ISSN: 0304-3835 |
| ispartof | Cancer letters, 1978-01, Vol.4 (3), p.163-169 |
| issn | 0304-3835 1872-7980 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73973820 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Blood Platelets - microbiology Humans Inclusion Bodies, Viral Leukemia, Experimental - microbiology Mice Microscopy, Electron Preleukemia - enzymology Preleukemia - microbiology Preleukemia - ultrastructure Rauscher Virus - isolation & purification Retroviridae - enzymology Retroviridae - isolation & purification RNA-Directed DNA Polymerase - analysis Spleen - microbiology |
| title | A combined assay for the rapid preliminary detection of structural retroviruses |
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