X-Ray Inhibition of DNA Synthesis at Discrete Times during S Phase in Synchronous Human Diploid Fibroblasts
Synchronous human diploid fibroblasts were exposed to 1000 rad of X rays at various times in S phase, and thymidine incorporation, an indicator of DNA synthesis, was measured throughout the cell cycle. When cells were irradiated just before the time when thymidine incorporation peaked in control cel...
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| Veröffentlicht in: | Radiat. Res.; (United States) 1982-02, Vol.89 (2), p.424-427 |
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| container_title | Radiat. Res.; (United States) |
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| creator | Kapp, L. N. Painter, R. B. |
| description | Synchronous human diploid fibroblasts were exposed to 1000 rad of X rays at various times in S phase, and thymidine incorporation, an indicator of DNA synthesis, was measured throughout the cell cycle. When cells were irradiated just before the time when thymidine incorporation peaked in control cells, the peak was strongly suppressed. Irradiation after appearance of an incorporation peak in control cells resulted in some depression of thymidine incorporation but had much less effect than irradiation before the peak. These results are in agreement with the interpretation that, in human diploid fibroblasts, most replicons initiate during relatively restricted times in S phase, leading to the multiple thymidine incorporation peaks observed in these cells. |
| doi_str_mv | 10.2307/3575787 |
| format | Article |
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These results are in agreement with the interpretation that, in human diploid fibroblasts, most replicons initiate during relatively restricted times in S phase, leading to the multiple thymidine incorporation peaks observed in these cells.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.2307/3575787</identifier><identifier>PMID: 7063624</identifier><language>eng</language><publisher>United States: Academic Press, Inc</publisher><subject>560121 - Radiation Effects on Cells- External Source- (-1987) ; ANIMAL CELLS ; AZINES ; BIOLOGICAL EFFECTS ; BIOLOGICAL RADIATION EFFECTS ; BIOSYNTHESIS ; Cell cycle ; Cell Division ; Cell lines ; CONNECTIVE TISSUE CELLS ; Correspondence ; Diploidy ; DNA ; DNA - biosynthesis ; DOSES ; ELECTROMAGNETIC RADIATION ; FIBROBLASTS ; Fibroblasts - radiation effects ; HeLa cells ; HETEROCYCLIC COMPOUNDS ; Humans ; Interphase ; IONIZING RADIATIONS ; Irradiation ; L cells ; Mitosis ; NUCLEIC ACIDS ; NUCLEOSIDES ; NUCLEOTIDES ; ORGANIC COMPOUNDS ; ORGANIC NITROGEN COMPOUNDS ; PYRIMIDINES ; RADIATION DOSES ; RADIATION EFFECTS ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIATIONS ; Replicon ; RIBOSIDES ; SOMATIC CELLS ; Space life sciences ; SYNTHESIS ; THYMIDINE ; Time Factors ; X RADIATION</subject><ispartof>Radiat. 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N.</creatorcontrib><creatorcontrib>Painter, R. B.</creatorcontrib><creatorcontrib>Univ. of California, San Francisco</creatorcontrib><title>X-Ray Inhibition of DNA Synthesis at Discrete Times during S Phase in Synchronous Human Diploid Fibroblasts</title><title>Radiat. Res.; (United States)</title><addtitle>Radiat Res</addtitle><description>Synchronous human diploid fibroblasts were exposed to 1000 rad of X rays at various times in S phase, and thymidine incorporation, an indicator of DNA synthesis, was measured throughout the cell cycle. When cells were irradiated just before the time when thymidine incorporation peaked in control cells, the peak was strongly suppressed. Irradiation after appearance of an incorporation peak in control cells resulted in some depression of thymidine incorporation but had much less effect than irradiation before the peak. These results are in agreement with the interpretation that, in human diploid fibroblasts, most replicons initiate during relatively restricted times in S phase, leading to the multiple thymidine incorporation peaks observed in these cells.</description><subject>560121 - Radiation Effects on Cells- External Source- (-1987)</subject><subject>ANIMAL CELLS</subject><subject>AZINES</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>BIOSYNTHESIS</subject><subject>Cell cycle</subject><subject>Cell Division</subject><subject>Cell lines</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>Correspondence</subject><subject>Diploidy</subject><subject>DNA</subject><subject>DNA - biosynthesis</subject><subject>DOSES</subject><subject>ELECTROMAGNETIC RADIATION</subject><subject>FIBROBLASTS</subject><subject>Fibroblasts - radiation effects</subject><subject>HeLa cells</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>Humans</subject><subject>Interphase</subject><subject>IONIZING RADIATIONS</subject><subject>Irradiation</subject><subject>L cells</subject><subject>Mitosis</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOSIDES</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC NITROGEN COMPOUNDS</subject><subject>PYRIMIDINES</subject><subject>RADIATION DOSES</subject><subject>RADIATION EFFECTS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS</subject><subject>Replicon</subject><subject>RIBOSIDES</subject><subject>SOMATIC CELLS</subject><subject>Space life sciences</subject><subject>SYNTHESIS</subject><subject>THYMIDINE</subject><subject>Time Factors</subject><subject>X RADIATION</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1LHTEUhoO02Fsr_oJCKMWups3kJJPMUvyoglSpFtwNSSbjRGeS2ySzuP--kXtxJ10dDuc5Lzy8CB3V5DsFIn4AF1xIsYdWdQuy4oywd2hFCEAluBQf0MeUnkjZ66bdR_uCNNBQtkLPD9VvtcFXfnTaZRc8DgM--3WC7zY-jza5hFXGZy6ZaLPF9262CfdLdP4R3-HbUSWLnX-hzRiDD0vCl8usfHlZT8H1-MLpGPSkUk6f0PtBTcke7uYB-nNxfn96WV3f_Lw6PbmuDDSQKwFKGjG00NRcSq2EBgaNoJK1FnSvda-4oqpoWuCs6XvGqCBDT4s51S2HA_RlmxtSdl0yLlszmuC9NbnjDIgkskDHW2gdw9_FptzNRdJOk_K2WHQCWkFrBv8Fa85rwggt4LctaGJIKdqhW0c3q7jpatK9lNTtSirk513komfbv3K7Vsr96_b-lHKIb8b8A4wplQk</recordid><startdate>198202</startdate><enddate>198202</enddate><creator>Kapp, L. N.</creator><creator>Painter, R. B.</creator><general>Academic Press, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T2</scope><scope>7TV</scope><scope>7U2</scope><scope>C1K</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>198202</creationdate><title>X-Ray Inhibition of DNA Synthesis at Discrete Times during S Phase in Synchronous Human Diploid Fibroblasts</title><author>Kapp, L. N. ; Painter, R. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-73a8c7f9361588ba7b343672849e3bdbbda5a2a938e3546dd44270fd21932b953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>560121 - Radiation Effects on Cells- External Source- (-1987)</topic><topic>ANIMAL CELLS</topic><topic>AZINES</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>BIOSYNTHESIS</topic><topic>Cell cycle</topic><topic>Cell Division</topic><topic>Cell lines</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>Correspondence</topic><topic>Diploidy</topic><topic>DNA</topic><topic>DNA - biosynthesis</topic><topic>DOSES</topic><topic>ELECTROMAGNETIC RADIATION</topic><topic>FIBROBLASTS</topic><topic>Fibroblasts - radiation effects</topic><topic>HeLa cells</topic><topic>HETEROCYCLIC COMPOUNDS</topic><topic>Humans</topic><topic>Interphase</topic><topic>IONIZING RADIATIONS</topic><topic>Irradiation</topic><topic>L cells</topic><topic>Mitosis</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOSIDES</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC NITROGEN COMPOUNDS</topic><topic>PYRIMIDINES</topic><topic>RADIATION DOSES</topic><topic>RADIATION EFFECTS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIATIONS</topic><topic>Replicon</topic><topic>RIBOSIDES</topic><topic>SOMATIC CELLS</topic><topic>Space life sciences</topic><topic>SYNTHESIS</topic><topic>THYMIDINE</topic><topic>Time Factors</topic><topic>X RADIATION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kapp, L. N.</creatorcontrib><creatorcontrib>Painter, R. B.</creatorcontrib><creatorcontrib>Univ. of California, San Francisco</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Pollution Abstracts</collection><collection>Safety Science and Risk</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Radiat. Res.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kapp, L. N.</au><au>Painter, R. B.</au><aucorp>Univ. of California, San Francisco</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>X-Ray Inhibition of DNA Synthesis at Discrete Times during S Phase in Synchronous Human Diploid Fibroblasts</atitle><jtitle>Radiat. Res.; (United States)</jtitle><addtitle>Radiat Res</addtitle><date>1982-02</date><risdate>1982</risdate><volume>89</volume><issue>2</issue><spage>424</spage><epage>427</epage><pages>424-427</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><abstract>Synchronous human diploid fibroblasts were exposed to 1000 rad of X rays at various times in S phase, and thymidine incorporation, an indicator of DNA synthesis, was measured throughout the cell cycle. When cells were irradiated just before the time when thymidine incorporation peaked in control cells, the peak was strongly suppressed. Irradiation after appearance of an incorporation peak in control cells resulted in some depression of thymidine incorporation but had much less effect than irradiation before the peak. These results are in agreement with the interpretation that, in human diploid fibroblasts, most replicons initiate during relatively restricted times in S phase, leading to the multiple thymidine incorporation peaks observed in these cells.</abstract><cop>United States</cop><pub>Academic Press, Inc</pub><pmid>7063624</pmid><doi>10.2307/3575787</doi><tpages>4</tpages></addata></record> |
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| ispartof | Radiat. Res.; (United States), 1982-02, Vol.89 (2), p.424-427 |
| issn | 0033-7587 1938-5404 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE |
| subjects | 560121 - Radiation Effects on Cells- External Source- (-1987) ANIMAL CELLS AZINES BIOLOGICAL EFFECTS BIOLOGICAL RADIATION EFFECTS BIOSYNTHESIS Cell cycle Cell Division Cell lines CONNECTIVE TISSUE CELLS Correspondence Diploidy DNA DNA - biosynthesis DOSES ELECTROMAGNETIC RADIATION FIBROBLASTS Fibroblasts - radiation effects HeLa cells HETEROCYCLIC COMPOUNDS Humans Interphase IONIZING RADIATIONS Irradiation L cells Mitosis NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS PYRIMIDINES RADIATION DOSES RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS Replicon RIBOSIDES SOMATIC CELLS Space life sciences SYNTHESIS THYMIDINE Time Factors X RADIATION |
| title | X-Ray Inhibition of DNA Synthesis at Discrete Times during S Phase in Synchronous Human Diploid Fibroblasts |
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