RANTES Expression and Contribution to Monocyte Chemotaxis in Arthritis
Rheumatoid arthritis (RA) is characterized by recruitment of leukocytes from the vasculature into inflamed synovial tissue (ST) and synovial fluid (SF), which depends, in part, upon the continued maintenance of chemotactic stimuli. RANTES is a potent chemoattractant for leukocytes including monocyte...
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| Veröffentlicht in: | Clinical immunology and immunopathology 1998-10, Vol.89 (1), p.44-53 |
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| container_title | Clinical immunology and immunopathology |
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| creator | Volin, Michael V. Shah, Manisha R. Tokuhira, Michihide Haines, G.Kenneth Woods, James M. Koch, Alisa E. |
| description | Rheumatoid arthritis (RA) is characterized by recruitment of leukocytes from the vasculature into inflamed synovial tissue (ST) and synovial fluid (SF), which depends, in part, upon the continued maintenance of chemotactic stimuli. RANTES is a potent chemoattractant for leukocytes including monocytes and CD45RO+memory T lymphocytes. The aim of this study was to determine the production, the source, and the function of antigenic RANTES in arthritis. We detected antigenic RANTES in SFs from RA and OA patients (100 ± 22.7 and 72 ± 30.7 pg/ml, respectively). CM from RA ST fibroblasts stimulated with interleukin-1β or tumor necrosis factor-α contained significantly more antigenic RANTES than unstimulated CM (452 ± 181.6 and 581 ± 200.2 pg/ml, respectively, versus 12 ± 4.4 pg/ml,P< 0.05). PHA-stimulated RA SF mononuclear cells secreted 5- to 15-fold more antigenic RANTES than did nonstimulated mononuclear cells, while LPS induced secretion up to 4-fold. We immunolocalized antigenic RANTES to sublining macrophages (28 ± 3.7 and 8 ± 2.0% immunopositive cells), perivascular macrophages (56 ± 6.9 and 19 ± 3.4%), and synovial lining cells (37 ± 5.8 and 60 ± 10.4%) in RA and OA tissue, respectively. Anti-RANTES neutralized 20.2 ± 1.3% of the RA SF chemotactic activity for normal peripheral blood monocytes (P< 0.05). These results demonstrate antigenic RANTES in RA and OA ST and SF and identify RANTES as a chemoattractant for monocytes in the RA joint. |
| doi_str_mv | 10.1006/clin.1998.4590 |
| format | Article |
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RANTES is a potent chemoattractant for leukocytes including monocytes and CD45RO+memory T lymphocytes. The aim of this study was to determine the production, the source, and the function of antigenic RANTES in arthritis. We detected antigenic RANTES in SFs from RA and OA patients (100 ± 22.7 and 72 ± 30.7 pg/ml, respectively). CM from RA ST fibroblasts stimulated with interleukin-1β or tumor necrosis factor-α contained significantly more antigenic RANTES than unstimulated CM (452 ± 181.6 and 581 ± 200.2 pg/ml, respectively, versus 12 ± 4.4 pg/ml,P< 0.05). PHA-stimulated RA SF mononuclear cells secreted 5- to 15-fold more antigenic RANTES than did nonstimulated mononuclear cells, while LPS induced secretion up to 4-fold. We immunolocalized antigenic RANTES to sublining macrophages (28 ± 3.7 and 8 ± 2.0% immunopositive cells), perivascular macrophages (56 ± 6.9 and 19 ± 3.4%), and synovial lining cells (37 ± 5.8 and 60 ± 10.4%) in RA and OA tissue, respectively. Anti-RANTES neutralized 20.2 ± 1.3% of the RA SF chemotactic activity for normal peripheral blood monocytes (P< 0.05). These results demonstrate antigenic RANTES in RA and OA ST and SF and identify RANTES as a chemoattractant for monocytes in the RA joint.</description><identifier>ISSN: 0090-1229</identifier><identifier>EISSN: 1090-2341</identifier><identifier>DOI: 10.1006/clin.1998.4590</identifier><identifier>PMID: 9756723</identifier><identifier>CODEN: CLIIAT</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Antigens - biosynthesis ; Arthritis - metabolism ; Arthritis, Rheumatoid - metabolism ; Arthritis, Rheumatoid - pathology ; Biological and medical sciences ; chemokine ; Chemokine CCL5 - biosynthesis ; Chemokine CCL5 - immunology ; chemotaxis ; Chemotaxis, Leukocyte - physiology ; Diseases of the osteoarticular system ; Fibroblasts - metabolism ; Humans ; Inflammatory joint diseases ; Interleukin-1 - pharmacology ; Lipopolysaccharides - pharmacology ; Medical sciences ; Monocytes - cytology ; Monocytes - metabolism ; Neutrophils - metabolism ; Osteoarthritis - metabolism ; Phytohemagglutinins - pharmacology ; Rheumatoid arthritis ; Synovial Fluid - chemistry ; Synovial Membrane - chemistry ; Synovial Membrane - metabolism ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Clinical immunology and immunopathology, 1998-10, Vol.89 (1), p.44-53</ispartof><rights>1998 Academic Press</rights><rights>1998 INIST-CNRS</rights><rights>Copyright 1998 Academic Press.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-229e5e5188bd5c3c12b97212efeff270225e5e16947afc0ee2bdb3849da38013</citedby><cites>FETCH-LOGICAL-c399t-229e5e5188bd5c3c12b97212efeff270225e5e16947afc0ee2bdb3849da38013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2410282$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9756723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Volin, Michael V.</creatorcontrib><creatorcontrib>Shah, Manisha R.</creatorcontrib><creatorcontrib>Tokuhira, Michihide</creatorcontrib><creatorcontrib>Haines, G.Kenneth</creatorcontrib><creatorcontrib>Woods, James M.</creatorcontrib><creatorcontrib>Koch, Alisa E.</creatorcontrib><title>RANTES Expression and Contribution to Monocyte Chemotaxis in Arthritis</title><title>Clinical immunology and immunopathology</title><addtitle>Clin Immunol Immunopathol</addtitle><description>Rheumatoid arthritis (RA) is characterized by recruitment of leukocytes from the vasculature into inflamed synovial tissue (ST) and synovial fluid (SF), which depends, in part, upon the continued maintenance of chemotactic stimuli. RANTES is a potent chemoattractant for leukocytes including monocytes and CD45RO+memory T lymphocytes. The aim of this study was to determine the production, the source, and the function of antigenic RANTES in arthritis. We detected antigenic RANTES in SFs from RA and OA patients (100 ± 22.7 and 72 ± 30.7 pg/ml, respectively). CM from RA ST fibroblasts stimulated with interleukin-1β or tumor necrosis factor-α contained significantly more antigenic RANTES than unstimulated CM (452 ± 181.6 and 581 ± 200.2 pg/ml, respectively, versus 12 ± 4.4 pg/ml,P< 0.05). PHA-stimulated RA SF mononuclear cells secreted 5- to 15-fold more antigenic RANTES than did nonstimulated mononuclear cells, while LPS induced secretion up to 4-fold. We immunolocalized antigenic RANTES to sublining macrophages (28 ± 3.7 and 8 ± 2.0% immunopositive cells), perivascular macrophages (56 ± 6.9 and 19 ± 3.4%), and synovial lining cells (37 ± 5.8 and 60 ± 10.4%) in RA and OA tissue, respectively. Anti-RANTES neutralized 20.2 ± 1.3% of the RA SF chemotactic activity for normal peripheral blood monocytes (P< 0.05). These results demonstrate antigenic RANTES in RA and OA ST and SF and identify RANTES as a chemoattractant for monocytes in the RA joint.</description><subject>Antigens - biosynthesis</subject><subject>Arthritis - metabolism</subject><subject>Arthritis, Rheumatoid - metabolism</subject><subject>Arthritis, Rheumatoid - pathology</subject><subject>Biological and medical sciences</subject><subject>chemokine</subject><subject>Chemokine CCL5 - biosynthesis</subject><subject>Chemokine CCL5 - immunology</subject><subject>chemotaxis</subject><subject>Chemotaxis, Leukocyte - physiology</subject><subject>Diseases of the osteoarticular system</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>Inflammatory joint diseases</subject><subject>Interleukin-1 - pharmacology</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Medical sciences</subject><subject>Monocytes - cytology</subject><subject>Monocytes - metabolism</subject><subject>Neutrophils - metabolism</subject><subject>Osteoarthritis - metabolism</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>Rheumatoid arthritis</subject><subject>Synovial Fluid - chemistry</subject><subject>Synovial Membrane - chemistry</subject><subject>Synovial Membrane - metabolism</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0090-1229</issn><issn>1090-2341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9LwzAUx4Moc06v3oQexNtmfrRLcxxjU2Eq6O4hTV9ZpE1mksn239uyspt4euF9P-_l8UHoluAJwXj6qGtjJ0SIfJJmAp-hIcECjylLyTka4u5NKBWX6CqEL9wOpJgP0EDwbMopG6Llx-xtvfhMFvuthxCMs4myZTJ3NnpT7GLXiC55ddbpQ4RkvoHGRbU3ITE2mfm48SaacI0uKlUHuOnrCK2Xi_X8ebx6f3qZz1ZjzYSI4_YSyCAjeV6UmWaa0EJwSihUUFWUY0rbFMhUpFxVGgPQoixYnopSsRwTNkIPx7Vb7753EKJsTNBQ18qC2wXJmeCECvYvSDjJ0hZtwckR1N6F4KGSW28a5Q-SYNkJlp1g2QmWneB24K7fvCsaKE94b7TN7_tcBa3qyiurTThhNCWY5t2_-RGD1taPAS-DNmA1lMaDjrJ05q8LfgGN_5YB</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>Volin, Michael V.</creator><creator>Shah, Manisha R.</creator><creator>Tokuhira, Michihide</creator><creator>Haines, G.Kenneth</creator><creator>Woods, James M.</creator><creator>Koch, Alisa E.</creator><general>Elsevier Inc</general><general>Academic Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19981001</creationdate><title>RANTES Expression and Contribution to Monocyte Chemotaxis in Arthritis</title><author>Volin, Michael V. ; Shah, Manisha R. ; Tokuhira, Michihide ; Haines, G.Kenneth ; Woods, James M. ; Koch, Alisa E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-229e5e5188bd5c3c12b97212efeff270225e5e16947afc0ee2bdb3849da38013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Antigens - biosynthesis</topic><topic>Arthritis - metabolism</topic><topic>Arthritis, Rheumatoid - metabolism</topic><topic>Arthritis, Rheumatoid - pathology</topic><topic>Biological and medical sciences</topic><topic>chemokine</topic><topic>Chemokine CCL5 - biosynthesis</topic><topic>Chemokine CCL5 - immunology</topic><topic>chemotaxis</topic><topic>Chemotaxis, Leukocyte - physiology</topic><topic>Diseases of the osteoarticular system</topic><topic>Fibroblasts - metabolism</topic><topic>Humans</topic><topic>Inflammatory joint diseases</topic><topic>Interleukin-1 - pharmacology</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Medical sciences</topic><topic>Monocytes - cytology</topic><topic>Monocytes - metabolism</topic><topic>Neutrophils - metabolism</topic><topic>Osteoarthritis - metabolism</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>Rheumatoid arthritis</topic><topic>Synovial Fluid - chemistry</topic><topic>Synovial Membrane - chemistry</topic><topic>Synovial Membrane - metabolism</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>online_resources</toplevel><creatorcontrib>Volin, Michael V.</creatorcontrib><creatorcontrib>Shah, Manisha R.</creatorcontrib><creatorcontrib>Tokuhira, Michihide</creatorcontrib><creatorcontrib>Haines, G.Kenneth</creatorcontrib><creatorcontrib>Woods, James M.</creatorcontrib><creatorcontrib>Koch, Alisa E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Volin, Michael V.</au><au>Shah, Manisha R.</au><au>Tokuhira, Michihide</au><au>Haines, G.Kenneth</au><au>Woods, James M.</au><au>Koch, Alisa E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RANTES Expression and Contribution to Monocyte Chemotaxis in Arthritis</atitle><jtitle>Clinical immunology and immunopathology</jtitle><addtitle>Clin Immunol Immunopathol</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>89</volume><issue>1</issue><spage>44</spage><epage>53</epage><pages>44-53</pages><issn>0090-1229</issn><eissn>1090-2341</eissn><coden>CLIIAT</coden><abstract>Rheumatoid arthritis (RA) is characterized by recruitment of leukocytes from the vasculature into inflamed synovial tissue (ST) and synovial fluid (SF), which depends, in part, upon the continued maintenance of chemotactic stimuli. RANTES is a potent chemoattractant for leukocytes including monocytes and CD45RO+memory T lymphocytes. The aim of this study was to determine the production, the source, and the function of antigenic RANTES in arthritis. We detected antigenic RANTES in SFs from RA and OA patients (100 ± 22.7 and 72 ± 30.7 pg/ml, respectively). CM from RA ST fibroblasts stimulated with interleukin-1β or tumor necrosis factor-α contained significantly more antigenic RANTES than unstimulated CM (452 ± 181.6 and 581 ± 200.2 pg/ml, respectively, versus 12 ± 4.4 pg/ml,P< 0.05). PHA-stimulated RA SF mononuclear cells secreted 5- to 15-fold more antigenic RANTES than did nonstimulated mononuclear cells, while LPS induced secretion up to 4-fold. We immunolocalized antigenic RANTES to sublining macrophages (28 ± 3.7 and 8 ± 2.0% immunopositive cells), perivascular macrophages (56 ± 6.9 and 19 ± 3.4%), and synovial lining cells (37 ± 5.8 and 60 ± 10.4%) in RA and OA tissue, respectively. Anti-RANTES neutralized 20.2 ± 1.3% of the RA SF chemotactic activity for normal peripheral blood monocytes (P< 0.05). These results demonstrate antigenic RANTES in RA and OA ST and SF and identify RANTES as a chemoattractant for monocytes in the RA joint.</abstract><cop>San Diego, CA</cop><cop>New York, NY</cop><cop>Boston</cop><pub>Elsevier Inc</pub><pmid>9756723</pmid><doi>10.1006/clin.1998.4590</doi><tpages>10</tpages></addata></record> |
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| subjects | Antigens - biosynthesis Arthritis - metabolism Arthritis, Rheumatoid - metabolism Arthritis, Rheumatoid - pathology Biological and medical sciences chemokine Chemokine CCL5 - biosynthesis Chemokine CCL5 - immunology chemotaxis Chemotaxis, Leukocyte - physiology Diseases of the osteoarticular system Fibroblasts - metabolism Humans Inflammatory joint diseases Interleukin-1 - pharmacology Lipopolysaccharides - pharmacology Medical sciences Monocytes - cytology Monocytes - metabolism Neutrophils - metabolism Osteoarthritis - metabolism Phytohemagglutinins - pharmacology Rheumatoid arthritis Synovial Fluid - chemistry Synovial Membrane - chemistry Synovial Membrane - metabolism Tumor Necrosis Factor-alpha - pharmacology |
| title | RANTES Expression and Contribution to Monocyte Chemotaxis in Arthritis |
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