A simple, low-cost and fast Peltier thermoregulation set-up for electrophysiology
Most of the parameters recorded in electrophysiology are strongly temperature dependent. In order to control temperature fluctuations we have built a system that ensures an accurate thermoregulation of the recording chamber. Temperature of physiological preparations can be changed relatively quickly...
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| Veröffentlicht in: | Journal of neuroscience methods 1998-09, Vol.83 (2), p.177-184 |
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| container_title | Journal of neuroscience methods |
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| creator | Corrèges, P. Bugnard, E. Millerin, C. Masiero, A. Andrivet, J.P. Bloc, A. Dunant, Y. |
| description | Most of the parameters recorded in electrophysiology are strongly temperature dependent. In order to control temperature fluctuations we have built a system that ensures an accurate thermoregulation of the recording chamber. Temperature of physiological preparations can be changed relatively quickly (about 8°C/min) and with a good accuracy (±0.5°C) without inducing thermal oscillations. Contrary to other thermoregulating devices, the temperature regulation is not carried out through the perfused medium but directly at the bottom of the chamber where a 3-cm
2 Peltier element has been placed. The element is driven by a dedicated electronic device which controls the amount and the direction of the current flowing across the Peltier thermocouple. All construction details and the appropriate electrical circuits are provided. Using this home-made device, the steady-state chamber temperature could be precisely monitored with a resolution of ±0.1°C in a range of 0–40°C. This set-up was tested in experiments designed to evaluate the temperature dependence of synaptic transmission in the
Torpedo nerve–electroplate synapses and of calcium currents recorded from isolated nerve cells. This low-cost method is suitable for a wide range of applications. |
| doi_str_mv | 10.1016/S0165-0270(98)00079-X |
| format | Article |
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2 Peltier element has been placed. The element is driven by a dedicated electronic device which controls the amount and the direction of the current flowing across the Peltier thermocouple. All construction details and the appropriate electrical circuits are provided. Using this home-made device, the steady-state chamber temperature could be precisely monitored with a resolution of ±0.1°C in a range of 0–40°C. This set-up was tested in experiments designed to evaluate the temperature dependence of synaptic transmission in the
Torpedo nerve–electroplate synapses and of calcium currents recorded from isolated nerve cells. This low-cost method is suitable for a wide range of applications.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/S0165-0270(98)00079-X</identifier><identifier>PMID: 9765131</identifier><identifier>CODEN: JNMEDT</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Barium - metabolism ; Biological and medical sciences ; Calcium channel ; Calcium Channels - physiology ; Costs and Cost Analysis ; Electric Organ - chemistry ; Electric Organ - physiology ; Electric Stimulation ; Electrophysiology ; Electrophysiology - economics ; Electrophysiology - instrumentation ; Electrophysiology - methods ; Fundamental and applied biological sciences. Psychology ; General aspects. Models. Methods ; Hybridomas ; Neuroblastoma ; Organ Culture Techniques ; Peltier effect ; Rats ; Synaptic transmission ; Synaptic Transmission - physiology ; Temperature ; Temperature control ; Torpedo ; Tumor Cells, Cultured - chemistry ; Tumor Cells, Cultured - physiology ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neuroscience methods, 1998-09, Vol.83 (2), p.177-184</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-575b21a4f23fb78747edd96ba04f607f387cce1c138b97bd5f6b46d13bddb7043</citedby><cites>FETCH-LOGICAL-c389t-575b21a4f23fb78747edd96ba04f607f387cce1c138b97bd5f6b46d13bddb7043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0165-0270(98)00079-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2398016$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9765131$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Corrèges, P.</creatorcontrib><creatorcontrib>Bugnard, E.</creatorcontrib><creatorcontrib>Millerin, C.</creatorcontrib><creatorcontrib>Masiero, A.</creatorcontrib><creatorcontrib>Andrivet, J.P.</creatorcontrib><creatorcontrib>Bloc, A.</creatorcontrib><creatorcontrib>Dunant, Y.</creatorcontrib><title>A simple, low-cost and fast Peltier thermoregulation set-up for electrophysiology</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>Most of the parameters recorded in electrophysiology are strongly temperature dependent. In order to control temperature fluctuations we have built a system that ensures an accurate thermoregulation of the recording chamber. Temperature of physiological preparations can be changed relatively quickly (about 8°C/min) and with a good accuracy (±0.5°C) without inducing thermal oscillations. Contrary to other thermoregulating devices, the temperature regulation is not carried out through the perfused medium but directly at the bottom of the chamber where a 3-cm
2 Peltier element has been placed. The element is driven by a dedicated electronic device which controls the amount and the direction of the current flowing across the Peltier thermocouple. All construction details and the appropriate electrical circuits are provided. Using this home-made device, the steady-state chamber temperature could be precisely monitored with a resolution of ±0.1°C in a range of 0–40°C. This set-up was tested in experiments designed to evaluate the temperature dependence of synaptic transmission in the
Torpedo nerve–electroplate synapses and of calcium currents recorded from isolated nerve cells. This low-cost method is suitable for a wide range of applications.</description><subject>Animals</subject><subject>Barium - metabolism</subject><subject>Biological and medical sciences</subject><subject>Calcium channel</subject><subject>Calcium Channels - physiology</subject><subject>Costs and Cost Analysis</subject><subject>Electric Organ - chemistry</subject><subject>Electric Organ - physiology</subject><subject>Electric Stimulation</subject><subject>Electrophysiology</subject><subject>Electrophysiology - economics</subject><subject>Electrophysiology - instrumentation</subject><subject>Electrophysiology - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Models. Methods</subject><subject>Hybridomas</subject><subject>Neuroblastoma</subject><subject>Organ Culture Techniques</subject><subject>Peltier effect</subject><subject>Rats</subject><subject>Synaptic transmission</subject><subject>Synaptic Transmission - physiology</subject><subject>Temperature</subject><subject>Temperature control</subject><subject>Torpedo</subject><subject>Tumor Cells, Cultured - chemistry</subject><subject>Tumor Cells, Cultured - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkFtrFDEUgINY6lr9CYV5EKngaDKZyeVJSvEGhSoq7FvI5aSNZCZjMlPZf2_aXfbVl3MOnO9c-BA6J_gdwYS9_1HD0OKO4wsp3mCMuWy3T9CGCN61jIvtU7Q5Is_Q81J-V6iXmJ2iU8nZQCjZoO-XTQnjHOFtE9Pf1qayNHpyjde1-AZxCZCb5Q7ymDLcrlEvIU1NgaVd58an3EAEu-Q03-1KSDHd7l6gE69jgZeHfIZ-ffr48-pLe33z-evV5XVrqZBLO_DBdET3vqPecMF7Ds5JZjTuPcPcU8GtBWIJFUZy4wbPTM8cocY5w3FPz9Dr_d45pz8rlEWNoViIUU-Q1qI4lYyIYajgsAdtTqVk8GrOYdR5pwhWDyrVo0r14ElJoR5Vqm2dOz8cWM0I7jh1cFf7rw59XayOPuvJhnLEOipF3VuxD3sMqoz7qlMVG2Cy4EKu6pRL4T-P_APoQJFF</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>Corrèges, P.</creator><creator>Bugnard, E.</creator><creator>Millerin, C.</creator><creator>Masiero, A.</creator><creator>Andrivet, J.P.</creator><creator>Bloc, A.</creator><creator>Dunant, Y.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980901</creationdate><title>A simple, low-cost and fast Peltier thermoregulation set-up for electrophysiology</title><author>Corrèges, P. ; Bugnard, E. ; Millerin, C. ; Masiero, A. ; Andrivet, J.P. ; Bloc, A. ; Dunant, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-575b21a4f23fb78747edd96ba04f607f387cce1c138b97bd5f6b46d13bddb7043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Barium - metabolism</topic><topic>Biological and medical sciences</topic><topic>Calcium channel</topic><topic>Calcium Channels - physiology</topic><topic>Costs and Cost Analysis</topic><topic>Electric Organ - chemistry</topic><topic>Electric Organ - physiology</topic><topic>Electric Stimulation</topic><topic>Electrophysiology</topic><topic>Electrophysiology - economics</topic><topic>Electrophysiology - instrumentation</topic><topic>Electrophysiology - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Models. Methods</topic><topic>Hybridomas</topic><topic>Neuroblastoma</topic><topic>Organ Culture Techniques</topic><topic>Peltier effect</topic><topic>Rats</topic><topic>Synaptic transmission</topic><topic>Synaptic Transmission - physiology</topic><topic>Temperature</topic><topic>Temperature control</topic><topic>Torpedo</topic><topic>Tumor Cells, Cultured - chemistry</topic><topic>Tumor Cells, Cultured - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Corrèges, P.</creatorcontrib><creatorcontrib>Bugnard, E.</creatorcontrib><creatorcontrib>Millerin, C.</creatorcontrib><creatorcontrib>Masiero, A.</creatorcontrib><creatorcontrib>Andrivet, J.P.</creatorcontrib><creatorcontrib>Bloc, A.</creatorcontrib><creatorcontrib>Dunant, Y.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Corrèges, P.</au><au>Bugnard, E.</au><au>Millerin, C.</au><au>Masiero, A.</au><au>Andrivet, J.P.</au><au>Bloc, A.</au><au>Dunant, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple, low-cost and fast Peltier thermoregulation set-up for electrophysiology</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>1998-09-01</date><risdate>1998</risdate><volume>83</volume><issue>2</issue><spage>177</spage><epage>184</epage><pages>177-184</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>Most of the parameters recorded in electrophysiology are strongly temperature dependent. In order to control temperature fluctuations we have built a system that ensures an accurate thermoregulation of the recording chamber. Temperature of physiological preparations can be changed relatively quickly (about 8°C/min) and with a good accuracy (±0.5°C) without inducing thermal oscillations. Contrary to other thermoregulating devices, the temperature regulation is not carried out through the perfused medium but directly at the bottom of the chamber where a 3-cm
2 Peltier element has been placed. The element is driven by a dedicated electronic device which controls the amount and the direction of the current flowing across the Peltier thermocouple. All construction details and the appropriate electrical circuits are provided. Using this home-made device, the steady-state chamber temperature could be precisely monitored with a resolution of ±0.1°C in a range of 0–40°C. This set-up was tested in experiments designed to evaluate the temperature dependence of synaptic transmission in the
Torpedo nerve–electroplate synapses and of calcium currents recorded from isolated nerve cells. This low-cost method is suitable for a wide range of applications.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9765131</pmid><doi>10.1016/S0165-0270(98)00079-X</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Barium - metabolism Biological and medical sciences Calcium channel Calcium Channels - physiology Costs and Cost Analysis Electric Organ - chemistry Electric Organ - physiology Electric Stimulation Electrophysiology Electrophysiology - economics Electrophysiology - instrumentation Electrophysiology - methods Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Hybridomas Neuroblastoma Organ Culture Techniques Peltier effect Rats Synaptic transmission Synaptic Transmission - physiology Temperature Temperature control Torpedo Tumor Cells, Cultured - chemistry Tumor Cells, Cultured - physiology Vertebrates: nervous system and sense organs |
| title | A simple, low-cost and fast Peltier thermoregulation set-up for electrophysiology |
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