Collagen required for proliferation of cultured connective tissue cells but not their transformed counterparts
THE proline analogue, cis -hydroxyproline, when incorporated into collagen, prevents collagen secretion 1 and also inhibits the growth of fibroblasts in vitro 2 . We describe here studies on the mechanism of action of cis -hydroxyproline on cell proliferation of primary cultures of murine connective...
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| Veröffentlicht in: | Nature (London) 1978-04, Vol.272 (5654), p.622-624 |
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| creator | LIOTTA, L. A. VEMBU, D. KLEINMAN, H. K. MARTIN, G. R. BOONE, C. |
| description | THE proline analogue,
cis
-hydroxyproline, when incorporated into collagen, prevents collagen secretion
1
and also inhibits the growth of fibroblasts
in vitro
2
. We describe here studies on the mechanism of action of
cis
-hydroxyproline on cell proliferation of primary cultures of murine connective tissue cells and of their spontaneously transformed, tumorigenic counterparts. We find that the analogue inhibits the attachment, spreading and proliferation of the connective tissue cells and that these effects are completely reversed when the cells are grown on collagen-coated dishes. The tumorigenic cells are less affected by
cis
-hydroxyproline even though their collagen synthesis is inhibited to a similar extent to the connective tissue cells. These results suggest that the presence of a collagen matrix is required for the growth of some connective tissue cells
in vitro
but not for the growth of their tumorigenic counterpart. |
| doi_str_mv | 10.1038/272622a0 |
| format | Article |
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cis
-hydroxyproline, when incorporated into collagen, prevents collagen secretion
1
and also inhibits the growth of fibroblasts
in vitro
2
. We describe here studies on the mechanism of action of
cis
-hydroxyproline on cell proliferation of primary cultures of murine connective tissue cells and of their spontaneously transformed, tumorigenic counterparts. We find that the analogue inhibits the attachment, spreading and proliferation of the connective tissue cells and that these effects are completely reversed when the cells are grown on collagen-coated dishes. The tumorigenic cells are less affected by
cis
-hydroxyproline even though their collagen synthesis is inhibited to a similar extent to the connective tissue cells. These results suggest that the presence of a collagen matrix is required for the growth of some connective tissue cells
in vitro
but not for the growth of their tumorigenic counterpart.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/272622a0</identifier><identifier>PMID: 565469</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Cell Adhesion - drug effects ; Cell Division - drug effects ; Cell Movement - drug effects ; Cell Transformation, Neoplastic ; Cells, Cultured ; Collagen - biosynthesis ; Collagen - physiology ; Connective Tissue Cells ; Humanities and Social Sciences ; Hydroxyproline - pharmacology ; letter ; Mice ; multidisciplinary ; Science ; Science (multidisciplinary)</subject><ispartof>Nature (London), 1978-04, Vol.272 (5654), p.622-624</ispartof><rights>Springer Nature Limited 1978</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-55242c3470dc935d4347f756ea4aa6b4e8a7bb6ced8b04ccc085422392b836363</citedby><cites>FETCH-LOGICAL-c311t-55242c3470dc935d4347f756ea4aa6b4e8a7bb6ced8b04ccc085422392b836363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/272622a0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/272622a0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/565469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LIOTTA, L. A.</creatorcontrib><creatorcontrib>VEMBU, D.</creatorcontrib><creatorcontrib>KLEINMAN, H. K.</creatorcontrib><creatorcontrib>MARTIN, G. R.</creatorcontrib><creatorcontrib>BOONE, C.</creatorcontrib><title>Collagen required for proliferation of cultured connective tissue cells but not their transformed counterparts</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>THE proline analogue,
cis
-hydroxyproline, when incorporated into collagen, prevents collagen secretion
1
and also inhibits the growth of fibroblasts
in vitro
2
. We describe here studies on the mechanism of action of
cis
-hydroxyproline on cell proliferation of primary cultures of murine connective tissue cells and of their spontaneously transformed, tumorigenic counterparts. We find that the analogue inhibits the attachment, spreading and proliferation of the connective tissue cells and that these effects are completely reversed when the cells are grown on collagen-coated dishes. The tumorigenic cells are less affected by
cis
-hydroxyproline even though their collagen synthesis is inhibited to a similar extent to the connective tissue cells. These results suggest that the presence of a collagen matrix is required for the growth of some connective tissue cells
in vitro
but not for the growth of their tumorigenic counterpart.</description><subject>Animals</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cell Movement - drug effects</subject><subject>Cell Transformation, Neoplastic</subject><subject>Cells, Cultured</subject><subject>Collagen - biosynthesis</subject><subject>Collagen - physiology</subject><subject>Connective Tissue Cells</subject><subject>Humanities and Social Sciences</subject><subject>Hydroxyproline - pharmacology</subject><subject>letter</subject><subject>Mice</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkEtLAzEUhYP4qlXwB7jISnQxmmTymC6l-IKCG10PmcydOmWa1DwE_72pU91IFjdwzj3c7yB0TskNJWV1yxSTjGmyhyaUK1lwWal9NCGEVQWpSnmMTkJYEUIEVfwIHQopuJxNkJ27YdBLsNjDR-o9tLhzHm-8G_oOvI69s9h12KQhpq1qnLVgYv8JOPYhJMAGhiHgJkVsXcTxHXqPo9c25KD1z0ayEfxG-xhO0UGnhwBnuzlFbw_3r_OnYvHy-Dy_WxSmpDQWQjDOTMkVac2sFC3P304JCZprLRsOlVZNIw20VUO4MYZUgjNWzliTWfObossxN4N8JAixXvdhe6i24FKoVTkTkjKejVej0XgXgoeu3vh-rf1XTUm9bbb-bTZbL3aZqclcf8axyixfj3LIgl2Cr1cueZsp_0d9A1IXglo</recordid><startdate>19780413</startdate><enddate>19780413</enddate><creator>LIOTTA, L. A.</creator><creator>VEMBU, D.</creator><creator>KLEINMAN, H. K.</creator><creator>MARTIN, G. R.</creator><creator>BOONE, C.</creator><general>Nature Publishing Group UK</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19780413</creationdate><title>Collagen required for proliferation of cultured connective tissue cells but not their transformed counterparts</title><author>LIOTTA, L. A. ; VEMBU, D. ; KLEINMAN, H. K. ; MARTIN, G. R. ; BOONE, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-55242c3470dc935d4347f756ea4aa6b4e8a7bb6ced8b04ccc085422392b836363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Animals</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Cell Movement - drug effects</topic><topic>Cell Transformation, Neoplastic</topic><topic>Cells, Cultured</topic><topic>Collagen - biosynthesis</topic><topic>Collagen - physiology</topic><topic>Connective Tissue Cells</topic><topic>Humanities and Social Sciences</topic><topic>Hydroxyproline - pharmacology</topic><topic>letter</topic><topic>Mice</topic><topic>multidisciplinary</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LIOTTA, L. A.</creatorcontrib><creatorcontrib>VEMBU, D.</creatorcontrib><creatorcontrib>KLEINMAN, H. K.</creatorcontrib><creatorcontrib>MARTIN, G. R.</creatorcontrib><creatorcontrib>BOONE, C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LIOTTA, L. A.</au><au>VEMBU, D.</au><au>KLEINMAN, H. K.</au><au>MARTIN, G. R.</au><au>BOONE, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Collagen required for proliferation of cultured connective tissue cells but not their transformed counterparts</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1978-04-13</date><risdate>1978</risdate><volume>272</volume><issue>5654</issue><spage>622</spage><epage>624</epage><pages>622-624</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>THE proline analogue,
cis
-hydroxyproline, when incorporated into collagen, prevents collagen secretion
1
and also inhibits the growth of fibroblasts
in vitro
2
. We describe here studies on the mechanism of action of
cis
-hydroxyproline on cell proliferation of primary cultures of murine connective tissue cells and of their spontaneously transformed, tumorigenic counterparts. We find that the analogue inhibits the attachment, spreading and proliferation of the connective tissue cells and that these effects are completely reversed when the cells are grown on collagen-coated dishes. The tumorigenic cells are less affected by
cis
-hydroxyproline even though their collagen synthesis is inhibited to a similar extent to the connective tissue cells. These results suggest that the presence of a collagen matrix is required for the growth of some connective tissue cells
in vitro
but not for the growth of their tumorigenic counterpart.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>565469</pmid><doi>10.1038/272622a0</doi><tpages>3</tpages></addata></record> |
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| ispartof | Nature (London), 1978-04, Vol.272 (5654), p.622-624 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals; Nature Journals Online |
| subjects | Animals Cell Adhesion - drug effects Cell Division - drug effects Cell Movement - drug effects Cell Transformation, Neoplastic Cells, Cultured Collagen - biosynthesis Collagen - physiology Connective Tissue Cells Humanities and Social Sciences Hydroxyproline - pharmacology letter Mice multidisciplinary Science Science (multidisciplinary) |
| title | Collagen required for proliferation of cultured connective tissue cells but not their transformed counterparts |
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