The distribution of 10nm filaments and microtubules in endothelial cells during mitosis: Double-label immunofluorescence study
I have used fluorescence microscopy and antibodies to 10nm filaments and tubulin labelled with contrasting fluorochromes to compare the distribution of these proteins in endothelial cells during cell division. During interphase the two filament systems have entirely different distributions: The bulk...
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| Veröffentlicht in: | Cell Motility 1981, Vol.1 (4), p.417-431 |
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| creator | Blose, Stephen H. |
| description | I have used fluorescence microscopy and antibodies to 10nm filaments and tubulin labelled with contrasting fluorochromes to compare the distribution of these proteins in endothelial cells during cell division. During interphase the two filament systems have entirely different distributions: The bulk of the 10nm filaments form a ring that surrounds the cell center and nucleus and remains parallel to the substrate, while the microtubules radiate from the cell center to the cell's border. When the mitotic spindle replaces the radial microtubule pattern in mitosis, the spindle poles remain within—and in close proximity to—the ring of 10nm filaments. This was confirmed by electron microscopy which showed the ring and centrioles in the same plane separated by a distance of 300–400 nm. |
| doi_str_mv | 10.1002/cm.970010403 |
| format | Article |
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This was confirmed by electron microscopy which showed the ring and centrioles in the same plane separated by a distance of 300–400 nm.</description><identifier>ISSN: 0271-6585</identifier><identifier>EISSN: 1097-0169</identifier><identifier>DOI: 10.1002/cm.970010403</identifier><identifier>PMID: 6756642</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; cell center ; Cell Division ; Cells, Cultured ; centrioles ; Centrioles - ultrastructure ; Cytoplasm - ultrastructure ; Cytoskeleton - ultrastructure ; electron microscopy ; Fluorescent Antibody Technique ; Guinea Pigs ; Microscopy, Electron ; Microtubule-Associated Proteins ; Microtubules - ultrastructure ; Mitosis ; Proteins - metabolism ; scaffold ; spindle poles ; Tubulin - metabolism</subject><ispartof>Cell Motility, 1981, Vol.1 (4), p.417-431</ispartof><rights>Copyright © 1980 Wiley‐Liss, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3703-29b6c5be559d8a58c968c38cd52140f9f718bf4127d1ad87948cb467037f3ef63</citedby><cites>FETCH-LOGICAL-c3703-29b6c5be559d8a58c968c38cd52140f9f718bf4127d1ad87948cb467037f3ef63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcm.970010403$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcm.970010403$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,4010,27904,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6756642$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blose, Stephen H.</creatorcontrib><title>The distribution of 10nm filaments and microtubules in endothelial cells during mitosis: Double-label immunofluorescence study</title><title>Cell Motility</title><addtitle>Cell Motility</addtitle><description>I have used fluorescence microscopy and antibodies to 10nm filaments and tubulin labelled with contrasting fluorochromes to compare the distribution of these proteins in endothelial cells during cell division. During interphase the two filament systems have entirely different distributions: The bulk of the 10nm filaments form a ring that surrounds the cell center and nucleus and remains parallel to the substrate, while the microtubules radiate from the cell center to the cell's border. When the mitotic spindle replaces the radial microtubule pattern in mitosis, the spindle poles remain within—and in close proximity to—the ring of 10nm filaments. This was confirmed by electron microscopy which showed the ring and centrioles in the same plane separated by a distance of 300–400 nm.</description><subject>Animals</subject><subject>cell center</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>centrioles</subject><subject>Centrioles - ultrastructure</subject><subject>Cytoplasm - ultrastructure</subject><subject>Cytoskeleton - ultrastructure</subject><subject>electron microscopy</subject><subject>Fluorescent Antibody Technique</subject><subject>Guinea Pigs</subject><subject>Microscopy, Electron</subject><subject>Microtubule-Associated Proteins</subject><subject>Microtubules - ultrastructure</subject><subject>Mitosis</subject><subject>Proteins - metabolism</subject><subject>scaffold</subject><subject>spindle poles</subject><subject>Tubulin - metabolism</subject><issn>0271-6585</issn><issn>1097-0169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kT1vFDEURS0ECkugo0VyRcUEezwej-nQhgRQApESRGl57Gdi8EcyHitsw29nol2tUqVy8c49er4PodeUHFFC2vcmHklBCCUdYU_QihIpGkJ7-RStSCto0_OBP0cvSvlNSCcEIwfooBe877t2hf5dXQO2vsyTH-vsc8LZYUpSxM4HHSHNBetkcfRmynMda4CCfcKQbJ6vIXgdsIEQCrZ18unXAs65-PIBH-c6BmiCHiFgH2NN2YWaJygGkgFc5mo3L9Ezp0OBV7v3EP04-XS1_tycfT_9sv541hgmCGtaOfaGj8C5tIPmg5H9YNhgLG9pR5x0gg6j62grLNV2ELIbzNj1S1Q4Bq5nh-jt1nsz5dsKZVbRl_u9dYJcixJMck4ZW8B3W3D5bikTOHUz-ainjaJE3detTFT7uhf8zc5bxwh2D-_6XeZ8O7_zATaPutT6_KG32eaWy8DffU5PfxY1E1z9_Haqvq6PGb24lOqc_Qfxd5tR</recordid><startdate>1981</startdate><enddate>1981</enddate><creator>Blose, Stephen H.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1981</creationdate><title>The distribution of 10nm filaments and microtubules in endothelial cells during mitosis: Double-label immunofluorescence study</title><author>Blose, Stephen H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3703-29b6c5be559d8a58c968c38cd52140f9f718bf4127d1ad87948cb467037f3ef63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>cell center</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>centrioles</topic><topic>Centrioles - ultrastructure</topic><topic>Cytoplasm - ultrastructure</topic><topic>Cytoskeleton - ultrastructure</topic><topic>electron microscopy</topic><topic>Fluorescent Antibody Technique</topic><topic>Guinea Pigs</topic><topic>Microscopy, Electron</topic><topic>Microtubule-Associated Proteins</topic><topic>Microtubules - ultrastructure</topic><topic>Mitosis</topic><topic>Proteins - metabolism</topic><topic>scaffold</topic><topic>spindle poles</topic><topic>Tubulin - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Blose, Stephen H.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell Motility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blose, Stephen H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The distribution of 10nm filaments and microtubules in endothelial cells during mitosis: Double-label immunofluorescence study</atitle><jtitle>Cell Motility</jtitle><addtitle>Cell Motility</addtitle><date>1981</date><risdate>1981</risdate><volume>1</volume><issue>4</issue><spage>417</spage><epage>431</epage><pages>417-431</pages><issn>0271-6585</issn><eissn>1097-0169</eissn><abstract>I have used fluorescence microscopy and antibodies to 10nm filaments and tubulin labelled with contrasting fluorochromes to compare the distribution of these proteins in endothelial cells during cell division. During interphase the two filament systems have entirely different distributions: The bulk of the 10nm filaments form a ring that surrounds the cell center and nucleus and remains parallel to the substrate, while the microtubules radiate from the cell center to the cell's border. When the mitotic spindle replaces the radial microtubule pattern in mitosis, the spindle poles remain within—and in close proximity to—the ring of 10nm filaments. This was confirmed by electron microscopy which showed the ring and centrioles in the same plane separated by a distance of 300–400 nm.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>6756642</pmid><doi>10.1002/cm.970010403</doi><tpages>15</tpages></addata></record> |
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| identifier | ISSN: 0271-6585 |
| ispartof | Cell Motility, 1981, Vol.1 (4), p.417-431 |
| issn | 0271-6585 1097-0169 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals cell center Cell Division Cells, Cultured centrioles Centrioles - ultrastructure Cytoplasm - ultrastructure Cytoskeleton - ultrastructure electron microscopy Fluorescent Antibody Technique Guinea Pigs Microscopy, Electron Microtubule-Associated Proteins Microtubules - ultrastructure Mitosis Proteins - metabolism scaffold spindle poles Tubulin - metabolism |
| title | The distribution of 10nm filaments and microtubules in endothelial cells during mitosis: Double-label immunofluorescence study |
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