Competitive Binding of Xenobiotic Oestrogens to Rat Alpha-Fetoprotein and to Sex Steroid Binding Proteins in Human and Rainbow Trout (Oncorhynchus mykiss) Plasma
The ability of a variety of “environmental oestrogens” to compete with radiolabelled steroids to rat α-fetoprotein (AFP) and to sex steroid binding proteins was investigated in human and rainbow trout (Oncorhynchus mykiss) plasma. For [3H]oestradiol binding to AFP, diethylstilbestrol and 4-nonylphen...
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| Veröffentlicht in: | General and comparative endocrinology 1998-10, Vol.112 (1), p.89-95 |
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| creator | Milligan, Stuart R. Khan, Omar Nash, Marnie |
| description | The ability of a variety of “environmental oestrogens” to compete with radiolabelled steroids to rat α-fetoprotein (AFP) and to sex steroid binding proteins was investigated in human and rainbow trout (Oncorhynchus mykiss) plasma. For [3H]oestradiol binding to AFP, diethylstilbestrol and 4-nonylphenoxyacetic acid showed significant competition at concentrations about 100-fold greater than oestradiol (relative binding affinities ≈ 1% c.f. oestradiol). All other compounds (phytooestrogens: coumestrol, daidzein, genistein; others: 4-nonylphenol, 4-tert-octylphenol, 4-nonylphenoldiethoxylate, 4-tert-butylphenol, bisphenol-A (Bis-A), bis(2- ethylhexl)phthalate, dioctylphthalate, dibutyl phthalate, 2,4′DDT (op' enantiomer), 2,4′-DDE (mixed enantiomers), kepone) showed only very weak or no competition (relative binding affinities 1000 fold more than the natural ligand) of a few of the compounds showing any ability to displace the natural ligand. These results suggest that environmental oestrogenic agents are unlikely to produce biological effects by displacing endogenous steroids from plasma steroid binding proteins unless they are present in very high concentrations. |
| doi_str_mv | 10.1006/gcen.1998.7146 |
| format | Article |
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For [3H]oestradiol binding to AFP, diethylstilbestrol and 4-nonylphenoxyacetic acid showed significant competition at concentrations about 100-fold greater than oestradiol (relative binding affinities ≈ 1% c.f. oestradiol). All other compounds (phytooestrogens: coumestrol, daidzein, genistein; others: 4-nonylphenol, 4-tert-octylphenol, 4-nonylphenoldiethoxylate, 4-tert-butylphenol, bisphenol-A (Bis-A), bis(2- ethylhexl)phthalate, dioctylphthalate, dibutyl phthalate, 2,4′DDT (op' enantiomer), 2,4′-DDE (mixed enantiomers), kepone) showed only very weak or no competition (relative binding affinities <<0.1% c.f. oestradiol). The situation for both human and fish plasma was very similar, with only very high concentrations (>>1000 fold more than the natural ligand) of a few of the compounds showing any ability to displace the natural ligand. These results suggest that environmental oestrogenic agents are unlikely to produce biological effects by displacing endogenous steroids from plasma steroid binding proteins unless they are present in very high concentrations.</description><identifier>ISSN: 0016-6480</identifier><identifier>EISSN: 1095-6840</identifier><identifier>DOI: 10.1006/gcen.1998.7146</identifier><identifier>PMID: 9748407</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>alpha fetoprotein ; alpha-Fetoproteins - metabolism ; Animals ; Binding, Competitive ; Carrier Proteins - blood ; Dihydrotestosterone - blood ; environmental oestrogens ; Estradiol - blood ; Female ; Freshwater ; Humans ; Oncorhynchus mykiss ; Oncorhynchus mykiss - blood ; phytooestrogens ; Pregnancy ; rainbow trout ; Rats ; Sex Hormone-Binding Globulin - metabolism ; steroid binding proteins ; Testosterone - blood ; xenobiotic ; Xenobiotics - blood</subject><ispartof>General and comparative endocrinology, 1998-10, Vol.112 (1), p.89-95</ispartof><rights>1998 Academic Press</rights><rights>Copyright 1998 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-b6d55740e9f17e7d5937b2f02110ad54b5f3e8005660e194e401e71fff4b913e3</citedby><cites>FETCH-LOGICAL-c436t-b6d55740e9f17e7d5937b2f02110ad54b5f3e8005660e194e401e71fff4b913e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/gcen.1998.7146$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27913,27914,45984</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9748407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Milligan, Stuart R.</creatorcontrib><creatorcontrib>Khan, Omar</creatorcontrib><creatorcontrib>Nash, Marnie</creatorcontrib><title>Competitive Binding of Xenobiotic Oestrogens to Rat Alpha-Fetoprotein and to Sex Steroid Binding Proteins in Human and Rainbow Trout (Oncorhynchus mykiss) Plasma</title><title>General and comparative endocrinology</title><addtitle>Gen Comp Endocrinol</addtitle><description>The ability of a variety of “environmental oestrogens” to compete with radiolabelled steroids to rat α-fetoprotein (AFP) and to sex steroid binding proteins was investigated in human and rainbow trout (Oncorhynchus mykiss) plasma. For [3H]oestradiol binding to AFP, diethylstilbestrol and 4-nonylphenoxyacetic acid showed significant competition at concentrations about 100-fold greater than oestradiol (relative binding affinities ≈ 1% c.f. oestradiol). All other compounds (phytooestrogens: coumestrol, daidzein, genistein; others: 4-nonylphenol, 4-tert-octylphenol, 4-nonylphenoldiethoxylate, 4-tert-butylphenol, bisphenol-A (Bis-A), bis(2- ethylhexl)phthalate, dioctylphthalate, dibutyl phthalate, 2,4′DDT (op' enantiomer), 2,4′-DDE (mixed enantiomers), kepone) showed only very weak or no competition (relative binding affinities <<0.1% c.f. oestradiol). The situation for both human and fish plasma was very similar, with only very high concentrations (>>1000 fold more than the natural ligand) of a few of the compounds showing any ability to displace the natural ligand. These results suggest that environmental oestrogenic agents are unlikely to produce biological effects by displacing endogenous steroids from plasma steroid binding proteins unless they are present in very high concentrations.</description><subject>alpha fetoprotein</subject><subject>alpha-Fetoproteins - metabolism</subject><subject>Animals</subject><subject>Binding, Competitive</subject><subject>Carrier Proteins - blood</subject><subject>Dihydrotestosterone - blood</subject><subject>environmental oestrogens</subject><subject>Estradiol - blood</subject><subject>Female</subject><subject>Freshwater</subject><subject>Humans</subject><subject>Oncorhynchus mykiss</subject><subject>Oncorhynchus mykiss - blood</subject><subject>phytooestrogens</subject><subject>Pregnancy</subject><subject>rainbow trout</subject><subject>Rats</subject><subject>Sex Hormone-Binding Globulin - metabolism</subject><subject>steroid binding proteins</subject><subject>Testosterone - blood</subject><subject>xenobiotic</subject><subject>Xenobiotics - blood</subject><issn>0016-6480</issn><issn>1095-6840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi1EVZaWKzcknxAcsh1vHDs5lhWlSJW2aovEzXKcya4hsRfbKezj8KYkyqo31NMc_m9-aeYj5C2DJQMQF1uDbsmqqlxKxsULsmBQFZkoObwkCwAmMsFLeEVex_gDAIpcsFNyWkk-EnJB_q59v8dkk31E-sm6xrot9S39js7X1idr6AZjCn6LLtLk6Z1O9LLb73R2hcnvg09oHdWumcJ7_EPvEwZvm6ey2xmJdMSuh17P8J22rva_6UPwQ6IfNs74sDs4sxsi7Q8_bYwf6W2nY6_PyUmru4hvjvOMfLv6_LC-zm42X76uL28yw3ORslo0RSE5YNUyibIpqlzWqxZWjIFuCl4XbY7l-AAhAFnFkQNDydq25XXFcszPyPu5d7zp1zDerHobDXadduiHqGRerWQO_FmQSQZsBeUILmfQBB9jwFbtg-11OCgGapKnJnlqkqcmeePCu2PzUPfYPOFHW2NezjmOf3i0GFQ0Fp3BxgY0STXe_q_6H0_KqnQ</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>Milligan, Stuart R.</creator><creator>Khan, Omar</creator><creator>Nash, Marnie</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>19981001</creationdate><title>Competitive Binding of Xenobiotic Oestrogens to Rat Alpha-Fetoprotein and to Sex Steroid Binding Proteins in Human and Rainbow Trout (Oncorhynchus mykiss) Plasma</title><author>Milligan, Stuart R. ; Khan, Omar ; Nash, Marnie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-b6d55740e9f17e7d5937b2f02110ad54b5f3e8005660e194e401e71fff4b913e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>alpha fetoprotein</topic><topic>alpha-Fetoproteins - metabolism</topic><topic>Animals</topic><topic>Binding, Competitive</topic><topic>Carrier Proteins - blood</topic><topic>Dihydrotestosterone - blood</topic><topic>environmental oestrogens</topic><topic>Estradiol - blood</topic><topic>Female</topic><topic>Freshwater</topic><topic>Humans</topic><topic>Oncorhynchus mykiss</topic><topic>Oncorhynchus mykiss - blood</topic><topic>phytooestrogens</topic><topic>Pregnancy</topic><topic>rainbow trout</topic><topic>Rats</topic><topic>Sex Hormone-Binding Globulin - metabolism</topic><topic>steroid binding proteins</topic><topic>Testosterone - blood</topic><topic>xenobiotic</topic><topic>Xenobiotics - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Milligan, Stuart R.</creatorcontrib><creatorcontrib>Khan, Omar</creatorcontrib><creatorcontrib>Nash, Marnie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>General and comparative endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Milligan, Stuart R.</au><au>Khan, Omar</au><au>Nash, Marnie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Competitive Binding of Xenobiotic Oestrogens to Rat Alpha-Fetoprotein and to Sex Steroid Binding Proteins in Human and Rainbow Trout (Oncorhynchus mykiss) Plasma</atitle><jtitle>General and comparative endocrinology</jtitle><addtitle>Gen Comp Endocrinol</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>112</volume><issue>1</issue><spage>89</spage><epage>95</epage><pages>89-95</pages><issn>0016-6480</issn><eissn>1095-6840</eissn><abstract>The ability of a variety of “environmental oestrogens” to compete with radiolabelled steroids to rat α-fetoprotein (AFP) and to sex steroid binding proteins was investigated in human and rainbow trout (Oncorhynchus mykiss) plasma. For [3H]oestradiol binding to AFP, diethylstilbestrol and 4-nonylphenoxyacetic acid showed significant competition at concentrations about 100-fold greater than oestradiol (relative binding affinities ≈ 1% c.f. oestradiol). All other compounds (phytooestrogens: coumestrol, daidzein, genistein; others: 4-nonylphenol, 4-tert-octylphenol, 4-nonylphenoldiethoxylate, 4-tert-butylphenol, bisphenol-A (Bis-A), bis(2- ethylhexl)phthalate, dioctylphthalate, dibutyl phthalate, 2,4′DDT (op' enantiomer), 2,4′-DDE (mixed enantiomers), kepone) showed only very weak or no competition (relative binding affinities <<0.1% c.f. oestradiol). The situation for both human and fish plasma was very similar, with only very high concentrations (>>1000 fold more than the natural ligand) of a few of the compounds showing any ability to displace the natural ligand. These results suggest that environmental oestrogenic agents are unlikely to produce biological effects by displacing endogenous steroids from plasma steroid binding proteins unless they are present in very high concentrations.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9748407</pmid><doi>10.1006/gcen.1998.7146</doi><tpages>7</tpages></addata></record> |
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| subjects | alpha fetoprotein alpha-Fetoproteins - metabolism Animals Binding, Competitive Carrier Proteins - blood Dihydrotestosterone - blood environmental oestrogens Estradiol - blood Female Freshwater Humans Oncorhynchus mykiss Oncorhynchus mykiss - blood phytooestrogens Pregnancy rainbow trout Rats Sex Hormone-Binding Globulin - metabolism steroid binding proteins Testosterone - blood xenobiotic Xenobiotics - blood |
| title | Competitive Binding of Xenobiotic Oestrogens to Rat Alpha-Fetoprotein and to Sex Steroid Binding Proteins in Human and Rainbow Trout (Oncorhynchus mykiss) Plasma |
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