Phenanthroline-Cu complex-mediated chemiluminescence of DNA and its potential use in antioxidation evaluation
The chemiluminescence (CL) concomitant with phen-Cu 2+ /ascorbate/H 2O 2-induced DNA damage has been studied. The emission intensity increases linearly with increasing DNA concentration. The emission spectrum has a maximal wavelength at about 410 nm. The luminescence is inhibited by histone in a his...
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| Veröffentlicht in: | Journal of photochemistry and photobiology. B, Biology Biology, 1998-06, Vol.44 (1), p.63-68 |
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| container_end_page | 68 |
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| container_issue | 1 |
| container_start_page | 63 |
| container_title | Journal of photochemistry and photobiology. B, Biology |
| container_volume | 44 |
| creator | Ma, Wenjian Cao, En-Hua Zhang, Jian Qin, Jing-Fen |
| description | The chemiluminescence (CL) concomitant with phen-Cu
2+ /ascorbate/H
2O
2-induced DNA damage has been studied. The emission intensity increases linearly with increasing DNA concentration. The emission spectrum has a maximal wavelength at about 410 nm. The luminescence is inhibited by histone in a histone concentration-dependent manner. The CL is characteristic of guanine. Of all common kinds of bases and nucleotides, only guanine or guanine nucleotides can give rise to luminescence. The possibility of using the luminescence as a means of studying antioxidation related to DNA damage is discussed. Several kinds of well-defined antioxidants have been used as testing reagents and show that this method can not only evaluate the antioxidative effect but also distinguish different types of antioxidants. |
| doi_str_mv | 10.1016/S1011-1344(98)00120-1 |
| format | Article |
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2+ /ascorbate/H
2O
2-induced DNA damage has been studied. The emission intensity increases linearly with increasing DNA concentration. The emission spectrum has a maximal wavelength at about 410 nm. The luminescence is inhibited by histone in a histone concentration-dependent manner. The CL is characteristic of guanine. Of all common kinds of bases and nucleotides, only guanine or guanine nucleotides can give rise to luminescence. The possibility of using the luminescence as a means of studying antioxidation related to DNA damage is discussed. Several kinds of well-defined antioxidants have been used as testing reagents and show that this method can not only evaluate the antioxidative effect but also distinguish different types of antioxidants.</description><identifier>ISSN: 1011-1344</identifier><identifier>EISSN: 1873-2682</identifier><identifier>DOI: 10.1016/S1011-1344(98)00120-1</identifier><identifier>PMID: 9745728</identifier><language>eng</language><publisher>Switzerland: Elsevier B.V</publisher><subject>Antioxidants ; Antioxidants - pharmacology ; Chemiluminescence ; Copper - pharmacology ; DNA - chemistry ; DNA - drug effects ; DNA Damage ; Guanine ; Intercalating Agents - pharmacology ; Kinetics ; Luminescent Measurements ; Oxidative stress ; Phenanthroline-Cu 2+ complex ; Phenanthrolines - pharmacology ; Reactive oxygen species</subject><ispartof>Journal of photochemistry and photobiology. B, Biology, 1998-06, Vol.44 (1), p.63-68</ispartof><rights>1998 Elsevier Science S.A. All rights reserved</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-4541eefae451bf31f0d3f2734546b9f727893f96dcaf19ae132b852f58e846663</citedby><cites>FETCH-LOGICAL-c360t-4541eefae451bf31f0d3f2734546b9f727893f96dcaf19ae132b852f58e846663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1011134498001201$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9745728$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Wenjian</creatorcontrib><creatorcontrib>Cao, En-Hua</creatorcontrib><creatorcontrib>Zhang, Jian</creatorcontrib><creatorcontrib>Qin, Jing-Fen</creatorcontrib><title>Phenanthroline-Cu complex-mediated chemiluminescence of DNA and its potential use in antioxidation evaluation</title><title>Journal of photochemistry and photobiology. B, Biology</title><addtitle>J Photochem Photobiol B</addtitle><description>The chemiluminescence (CL) concomitant with phen-Cu
2+ /ascorbate/H
2O
2-induced DNA damage has been studied. The emission intensity increases linearly with increasing DNA concentration. The emission spectrum has a maximal wavelength at about 410 nm. The luminescence is inhibited by histone in a histone concentration-dependent manner. The CL is characteristic of guanine. Of all common kinds of bases and nucleotides, only guanine or guanine nucleotides can give rise to luminescence. The possibility of using the luminescence as a means of studying antioxidation related to DNA damage is discussed. Several kinds of well-defined antioxidants have been used as testing reagents and show that this method can not only evaluate the antioxidative effect but also distinguish different types of antioxidants.</description><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Chemiluminescence</subject><subject>Copper - pharmacology</subject><subject>DNA - chemistry</subject><subject>DNA - drug effects</subject><subject>DNA Damage</subject><subject>Guanine</subject><subject>Intercalating Agents - pharmacology</subject><subject>Kinetics</subject><subject>Luminescent Measurements</subject><subject>Oxidative stress</subject><subject>Phenanthroline-Cu 2+ complex</subject><subject>Phenanthrolines - pharmacology</subject><subject>Reactive oxygen species</subject><issn>1011-1344</issn><issn>1873-2682</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtP9CAUhonReBn9CSasjN-iCqWlsDJmPm-JURN1TRh6yGBaGAs1-u9lLrqVxbnwvnDgQeiYkjNKKD9_zpEWlFXVqRT_CKElKegW2qeiYUXJRbmd6x_LHjqI8Y3kVfNmF-3KpqqbUuyj_mkOXvs0H0LnPBTTEZvQLzr4LHponU7QYjOH3nVjn_VowBvAweL_D5dY-xa7FPEiJPDJ6Q6PEbDzWUgufLpW5-QxfOhuXJWHaMfqLsLRJk_Q6_XVy_S2uH-8uZte3heGcZKKqq4ogNVQ1XRmGbWkZbZsWN7nM2mbshGSWclboy2VGigrZ6IubS1AVJxzNkEn63sXQ3gfISbVu_z0rtMewhhVwyTlkotsrNdGM4QYB7BqMbheD1-KErXErFaY1ZKhkkKtMOdugo43A8ZZ5vR7asM16xdrHfIvPxwMKhq3RNe6AUxSbXB_TPgGXzuN5A</recordid><startdate>19980615</startdate><enddate>19980615</enddate><creator>Ma, Wenjian</creator><creator>Cao, En-Hua</creator><creator>Zhang, Jian</creator><creator>Qin, Jing-Fen</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980615</creationdate><title>Phenanthroline-Cu complex-mediated chemiluminescence of DNA and its potential use in antioxidation evaluation</title><author>Ma, Wenjian ; Cao, En-Hua ; Zhang, Jian ; Qin, Jing-Fen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-4541eefae451bf31f0d3f2734546b9f727893f96dcaf19ae132b852f58e846663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Chemiluminescence</topic><topic>Copper - pharmacology</topic><topic>DNA - chemistry</topic><topic>DNA - drug effects</topic><topic>DNA Damage</topic><topic>Guanine</topic><topic>Intercalating Agents - pharmacology</topic><topic>Kinetics</topic><topic>Luminescent Measurements</topic><topic>Oxidative stress</topic><topic>Phenanthroline-Cu 2+ complex</topic><topic>Phenanthrolines - pharmacology</topic><topic>Reactive oxygen species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Wenjian</creatorcontrib><creatorcontrib>Cao, En-Hua</creatorcontrib><creatorcontrib>Zhang, Jian</creatorcontrib><creatorcontrib>Qin, Jing-Fen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Wenjian</au><au>Cao, En-Hua</au><au>Zhang, Jian</au><au>Qin, Jing-Fen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenanthroline-Cu complex-mediated chemiluminescence of DNA and its potential use in antioxidation evaluation</atitle><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle><addtitle>J Photochem Photobiol B</addtitle><date>1998-06-15</date><risdate>1998</risdate><volume>44</volume><issue>1</issue><spage>63</spage><epage>68</epage><pages>63-68</pages><issn>1011-1344</issn><eissn>1873-2682</eissn><abstract>The chemiluminescence (CL) concomitant with phen-Cu
2+ /ascorbate/H
2O
2-induced DNA damage has been studied. The emission intensity increases linearly with increasing DNA concentration. The emission spectrum has a maximal wavelength at about 410 nm. The luminescence is inhibited by histone in a histone concentration-dependent manner. The CL is characteristic of guanine. Of all common kinds of bases and nucleotides, only guanine or guanine nucleotides can give rise to luminescence. The possibility of using the luminescence as a means of studying antioxidation related to DNA damage is discussed. Several kinds of well-defined antioxidants have been used as testing reagents and show that this method can not only evaluate the antioxidative effect but also distinguish different types of antioxidants.</abstract><cop>Switzerland</cop><pub>Elsevier B.V</pub><pmid>9745728</pmid><doi>10.1016/S1011-1344(98)00120-1</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 1011-1344 |
| ispartof | Journal of photochemistry and photobiology. B, Biology, 1998-06, Vol.44 (1), p.63-68 |
| issn | 1011-1344 1873-2682 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73916968 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antioxidants Antioxidants - pharmacology Chemiluminescence Copper - pharmacology DNA - chemistry DNA - drug effects DNA Damage Guanine Intercalating Agents - pharmacology Kinetics Luminescent Measurements Oxidative stress Phenanthroline-Cu 2+ complex Phenanthrolines - pharmacology Reactive oxygen species |
| title | Phenanthroline-Cu complex-mediated chemiluminescence of DNA and its potential use in antioxidation evaluation |
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