Quantification of cytokine mRNA in peripheral blood mononuclear cells using branched DNA (bDNA) technology

Changes in the patterns of cytokine expression are thought to be of central importance in human infectious and inflammatory diseases. As such, there is a need for precise, reproducible assays for quantification of cytokine mRNA that are amenable to routine use in a clinical setting. In this report,...

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Veröffentlicht in:	Journal of immunological methods 1998-06, Vol.215 (1), p.123-134
Hauptverfasser:	Shen, Lu-Ping, Sheridan, Pat, Cao, Wei W., Dailey, Peter J., Salazar-Gonzalez, Jesus F., Breen, Elizabeth Crabb, Fahey, John L., Urdea, Mickey S., Kolberg, Janice A.
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Analytical, structural and metabolic biochemistry Biological and medical sciences Branched DNA (bDNA) Cytokine mRNA quantification Cytokines - biosynthesis Cytokines - blood DNA - analysis Fundamental and applied biological sciences. Psychology Fundamental immunology HIV Seropositivity - blood HIV-1 - immunology Humans Leukocytes, Mononuclear - metabolism Linear Models Molecular immunology Nucleic Acid Conformation Oligonucleotide Probes Peripheral blood mononuclear cells Protein hormones. Growth factors. Cytokines Proteins Reproducibility of Results RNA, Messenger - blood Sensitivity and Specificity Techniques
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