Expression of enzymatically active, recombinant barley alpha-glucosidase in yeast and immunological detection of alpha-glucosidase from seed tissue

An α-glucosidase cDNA clone derived from barley aleurone tissue was expressed in Pichia pastoris and Escherichia coli. The gene was fused with the N-terminal region of the Saccharomyces cerevisiae α-factor secretory peptide and placed under control of the Pichia AOX1 promoter in the vector pPIC9. En...

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Veröffentlicht in:	Plant molecular biology 1998-10, Vol.38 (3), p.379-391
Hauptverfasser:	Tibbot, B.K. (Wisconsin Univ., Madison, WI (USA). Dept. of Agronomy), Henson, C.A, Skadsen, R.W
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN RECOMBINADO ADN RECOMBINE ALFA GLUCOSIDASA ALPHA GLUCOSIDASE alpha-Glucosidases - genetics alpha-Glucosidases - immunology alpha-Glucosidases - metabolism Amino Acid Sequence ANTICORPS POLYCLONAL ANTICUERPOS POLICLONALES barley Base Sequence complementary DNA DNA, Complementary - genetics DNA, Plant - genetics ESCHERICHIA COLI Escherichia coli - genetics EXPRESION GENICA EXPRESSION DES GENES GENE EXPRESSION genes GERMINACION GERMINATION gibberellic acid Hordeum - enzymology Hordeum - genetics HORDEUM VULGARE Immunochemistry isomaltose MALTOSA MALTOSE methanol Molecular Sequence Data Molecular Weight PICHIA Pichia - genetics Pichia pastoris POLYCLONAL ANTIBODIES proteins RECOMBINANT DNA Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism SACCHAROMYCES CEREVISIAE seed germination Seeds - enzymology trehalose yeasts
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