Human estrogen sulfotransferase (hEST1) activities and its mRNA in various breast cancer cell lines. Effect of the progestin, promegestone (R-5020)
Using reverse transcriptase-polymerase chain reaction amplification it was possible to detect the presence of type 1 human estrogen sulfotransferase (hEST1) mRNA in the hormone-dependent: MCF-7 and T-47D, and hormone-independent: MDA-MB-231 and MDA-MB-468, human breast cancer cells. The expression o...
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| Veröffentlicht in: | The Journal of steroid biochemistry and molecular biology 1998-09, Vol.66 (5), p.295-302 |
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| container_title | The Journal of steroid biochemistry and molecular biology |
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| creator | Chetrite, G Le Nestour, E Pasqualini, J.R |
| description | Using reverse transcriptase-polymerase chain reaction amplification it was possible to detect the presence of type 1 human estrogen sulfotransferase (hEST1) mRNA in the hormone-dependent: MCF-7 and T-47D, and hormone-independent: MDA-MB-231 and MDA-MB-468, human breast cancer cells. The expression of this mRNA is significantly higher in the MDA-MB-468 cells and a correlation of this mRNA expression with the enzymatic activity was observed. The progestin promegestone (R-5020) at a low concentration (5×10
−7
M) can significantly increase the estrogen sulfotransferase activity and its mRNA in the hormone-dependent MCF-7 and T-47D cells. As estrogen sulfates are biologically inactive, the stimulatory effect on sulfotransferase by promegestone may open attractive possibilities in the control of estradiol in human breast cancer. |
| doi_str_mv | 10.1016/S0960-0760(98)00063-6 |
| format | Article |
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−7
M) can significantly increase the estrogen sulfotransferase activity and its mRNA in the hormone-dependent MCF-7 and T-47D cells. As estrogen sulfates are biologically inactive, the stimulatory effect on sulfotransferase by promegestone may open attractive possibilities in the control of estradiol in human breast cancer.</description><identifier>ISSN: 0960-0760</identifier><identifier>EISSN: 1879-1220</identifier><identifier>DOI: 10.1016/S0960-0760(98)00063-6</identifier><identifier>PMID: 9749835</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Biological and medical sciences ; breast cancer ; Breast Neoplasms - enzymology ; Breast Neoplasms - genetics ; estrogens ; Estrone - metabolism ; Gene Expression Regulation, Enzymologic - drug effects ; Gene Expression Regulation, Enzymologic - physiology ; Gene Expression Regulation, Neoplastic - drug effects ; Gene Expression Regulation, Neoplastic - physiology ; Hormones. Endocrine system ; Humans ; Medical sciences ; mRNA ; Pharmacology. Drug treatments ; Promegestone - pharmacology ; RNA, Messenger - analysis ; RNA, Neoplasm - analysis ; sulfotransferase ; Sulfotransferases - genetics ; Sulfotransferases - metabolism ; Tumor Cells, Cultured</subject><ispartof>The Journal of steroid biochemistry and molecular biology, 1998-09, Vol.66 (5), p.295-302</ispartof><rights>1998 Elsevier Science Ltd</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-1303dfcdbb1d78bd0425d33e6c527f632bdb4082e60df91cd743f307ab17ed8e3</citedby><cites>FETCH-LOGICAL-c420t-1303dfcdbb1d78bd0425d33e6c527f632bdb4082e60df91cd743f307ab17ed8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960076098000636$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2403636$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9749835$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chetrite, G</creatorcontrib><creatorcontrib>Le Nestour, E</creatorcontrib><creatorcontrib>Pasqualini, J.R</creatorcontrib><title>Human estrogen sulfotransferase (hEST1) activities and its mRNA in various breast cancer cell lines. Effect of the progestin, promegestone (R-5020)</title><title>The Journal of steroid biochemistry and molecular biology</title><addtitle>J Steroid Biochem Mol Biol</addtitle><description>Using reverse transcriptase-polymerase chain reaction amplification it was possible to detect the presence of type 1 human estrogen sulfotransferase (hEST1) mRNA in the hormone-dependent: MCF-7 and T-47D, and hormone-independent: MDA-MB-231 and MDA-MB-468, human breast cancer cells. The expression of this mRNA is significantly higher in the MDA-MB-468 cells and a correlation of this mRNA expression with the enzymatic activity was observed. The progestin promegestone (R-5020) at a low concentration (5×10
−7
M) can significantly increase the estrogen sulfotransferase activity and its mRNA in the hormone-dependent MCF-7 and T-47D cells. As estrogen sulfates are biologically inactive, the stimulatory effect on sulfotransferase by promegestone may open attractive possibilities in the control of estradiol in human breast cancer.</description><subject>Biological and medical sciences</subject><subject>breast cancer</subject><subject>Breast Neoplasms - enzymology</subject><subject>Breast Neoplasms - genetics</subject><subject>estrogens</subject><subject>Estrone - metabolism</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Gene Expression Regulation, Neoplastic - physiology</subject><subject>Hormones. Endocrine system</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>mRNA</subject><subject>Pharmacology. Drug treatments</subject><subject>Promegestone - pharmacology</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Neoplasm - analysis</subject><subject>sulfotransferase</subject><subject>Sulfotransferases - genetics</subject><subject>Sulfotransferases - metabolism</subject><subject>Tumor Cells, Cultured</subject><issn>0960-0760</issn><issn>1879-1220</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdFqFDEUhoModa0-QiEXIl1w6kkyM5m5klJWKxSFtl6HTHJiIzOZmmQXfA5f2Ex32dteJeH85_x_zkfIGYMLBqz9dAd9CxXIFs77bg0ArajaF2TFOtlXjHN4SVZHyWvyJqXfRSQEkyfkpJd134lmRf5dbycdKKYc518YaNqObs5Rh-Qw6oT0_GFzd8_WVJvsdz57TFQHS31OdLr9fkl9oDsd_bxNdIioU6ZGB4ORGhxHOvqA6YJunEOT6exofkD6uFil7MPH5Trh8phDsbqtGuCwfkteOT0mfHc4T8nPL5v7q-vq5sfXb1eXN5WpOeSKCRDWGTsMzMpusFDzxgqBrWm4dK3ggx1q6Di2YF3PjJW1cAKkHphE26E4JR_2c0uKP9sSQk0-LbF1wPIfJUUPjeTwrJBJ1vUNZ0XY7IUmzilFdOox-knHv4qBWqipJ2pqQaL6Tj1RU23pOzsYbIcJ7bHrgKnU3x_qOhk9usLH-HSU8RpEK5Yxn_cyLFvbeYwqGY-FhvWx7F_Z2T8T5D8ZlrPR</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>Chetrite, G</creator><creator>Le Nestour, E</creator><creator>Pasqualini, J.R</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19980901</creationdate><title>Human estrogen sulfotransferase (hEST1) activities and its mRNA in various breast cancer cell lines. Effect of the progestin, promegestone (R-5020)</title><author>Chetrite, G ; Le Nestour, E ; Pasqualini, J.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-1303dfcdbb1d78bd0425d33e6c527f632bdb4082e60df91cd743f307ab17ed8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>breast cancer</topic><topic>Breast Neoplasms - enzymology</topic><topic>Breast Neoplasms - genetics</topic><topic>estrogens</topic><topic>Estrone - metabolism</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Gene Expression Regulation, Neoplastic - physiology</topic><topic>Hormones. Endocrine system</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>mRNA</topic><topic>Pharmacology. Drug treatments</topic><topic>Promegestone - pharmacology</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Neoplasm - analysis</topic><topic>sulfotransferase</topic><topic>Sulfotransferases - genetics</topic><topic>Sulfotransferases - metabolism</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chetrite, G</creatorcontrib><creatorcontrib>Le Nestour, E</creatorcontrib><creatorcontrib>Pasqualini, J.R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of steroid biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chetrite, G</au><au>Le Nestour, E</au><au>Pasqualini, J.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human estrogen sulfotransferase (hEST1) activities and its mRNA in various breast cancer cell lines. 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−7
M) can significantly increase the estrogen sulfotransferase activity and its mRNA in the hormone-dependent MCF-7 and T-47D cells. As estrogen sulfates are biologically inactive, the stimulatory effect on sulfotransferase by promegestone may open attractive possibilities in the control of estradiol in human breast cancer.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9749835</pmid><doi>10.1016/S0960-0760(98)00063-6</doi><tpages>8</tpages></addata></record> |
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| ispartof | The Journal of steroid biochemistry and molecular biology, 1998-09, Vol.66 (5), p.295-302 |
| issn | 0960-0760 1879-1220 |
| language | eng |
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| subjects | Biological and medical sciences breast cancer Breast Neoplasms - enzymology Breast Neoplasms - genetics estrogens Estrone - metabolism Gene Expression Regulation, Enzymologic - drug effects Gene Expression Regulation, Enzymologic - physiology Gene Expression Regulation, Neoplastic - drug effects Gene Expression Regulation, Neoplastic - physiology Hormones. Endocrine system Humans Medical sciences mRNA Pharmacology. Drug treatments Promegestone - pharmacology RNA, Messenger - analysis RNA, Neoplasm - analysis sulfotransferase Sulfotransferases - genetics Sulfotransferases - metabolism Tumor Cells, Cultured |
| title | Human estrogen sulfotransferase (hEST1) activities and its mRNA in various breast cancer cell lines. Effect of the progestin, promegestone (R-5020) |
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